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The map label for this gene is nth [H]

Identifier: 145299430

GI number: 145299430

Start: 2654051

End: 2654692

Strand: Direct

Name: nth [H]

Synonym: ASA_2491

Alternate gene names: 145299430

Gene position: 2654051-2654692 (Clockwise)

Preceding gene: 145299429

Following gene: 145299431

Centisome position: 56.44

GC content: 57.79

Gene sequence:

>642_bases
ATGAACAACCAGAAACGCAGGCAGATCCTGGAGCGACTGCGGGACAACAATCCCCATCCCACCACAGAGCTCAACTTCAC
CTCGCCCTTCGAGCTCTTGATTGCCGTGTTGCTGTCAGCCCAGGCCACCGACGTAAGCGTCAACAAGGCCACCGACAAGC
TCTATCCGGTGGCCAATACCCCGGCCGCCATGCTGGCGCTCGGGGTGGATGGGCTGAAACAGTACATCAAGACCATAGGC
CTGTTTAACACCAAGGCCGAAAACGTCATCAAGACGTGCGCCATCTTGCTGGAACGCCATGGCGGCGAGGTGCCGGAGAA
CCGCGAGGCGCTGGAAGCGCTGCCCGGTGTGGGTCGCAAGACCGCCAACGTGGTGCTCAATACCGCCTTTGGCTGGCCCA
CCATCGCCGTCGATACCCACATCTTTCGGGTGTCGAATCGCACCGGCTTCGCGGTGGGCAAAAACGTTGATCAGGTGGAA
GAGAAACTGCTGAAAGTGGTGCCTGCCGAGTTCAAGCTGGATGTGCACCACTGGTTGATCCTGCACGGCCGTTACACCTG
TCTGGCTCGCAAACCGCGCTGCGGCTCCTGTATCATTGAAGACTTGTGTGAATACAAAGAAAAAGTCTATCCACAGGAAT
AA

Upstream 100 bases:

>100_bases
TGACTGGCGTGATCGCAAGCAAGCCGCCCCCCTTGCCGATAGCAGTGTGTCTGTGGCGGGTGTGCACGCCACCCAGCTCT
GATCTGAGACGTGAGAAACC

Downstream 100 bases:

>100_bases
GGCAGCATGCAGGCTGTACGCTATAGCCCGCAAACTTCTGCTGCGATTCCTGTATCATCCAAGACTTGTGTGAGTACAAA
AATCTACCCGGAGAACTGAG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNNQKRRQILERLRDNNPHPTTELNFTSPFELLIAVLLSAQATDVSVNKATDKLYPVANTPAAMLALGVDGLKQYIKTIG
LFNTKAENVIKTCAILLERHGGEVPENREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVSNRTGFAVGKNVDQVE
EKLLKVVPAEFKLDVHHWLILHGRYTCLARKPRCGSCIIEDLCEYKEKVYPQE

Sequences:

>Translated_213_residues
MNNQKRRQILERLRDNNPHPTTELNFTSPFELLIAVLLSAQATDVSVNKATDKLYPVANTPAAMLALGVDGLKQYIKTIG
LFNTKAENVIKTCAILLERHGGEVPENREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVSNRTGFAVGKNVDQVE
EKLLKVVPAEFKLDVHHWLILHGRYTCLARKPRCGSCIIEDLCEYKEKVYPQE
>Mature_213_residues
MNNQKRRQILERLRDNNPHPTTELNFTSPFELLIAVLLSAQATDVSVNKATDKLYPVANTPAAMLALGVDGLKQYIKTIG
LFNTKAENVIKTCAILLERHGGEVPENREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVSNRTGFAVGKNVDQVE
EKLLKVVPAEFKLDVHHWLILHGRYTCLARKPRCGSCIIEDLCEYKEKVYPQE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=87.0813397129187, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=32, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=24.5989304812834, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550361, Length=190, Percent_Identity=32.6315789473684, Blast_Score=104, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23816; Mature: 23816

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQKRRQILERLRDNNPHPTTELNFTSPFELLIAVLLSAQATDVSVNKATDKLYPVANT
CCCHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCC
PAAMLALGVDGLKQYIKTIGLFNTKAENVIKTCAILLERHGGEVPENREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRVSNRTGFAVGKNVDQVEEKLLKVVPAEFKLDVHHWLI
HHHEEEECCCCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHEECHHHEEE
LHGRYTCLARKPRCGSCIIEDLCEYKEKVYPQE
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNQKRRQILERLRDNNPHPTTELNFTSPFELLIAVLLSAQATDVSVNKATDKLYPVANT
CCCHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCC
PAAMLALGVDGLKQYIKTIGLFNTKAENVIKTCAILLERHGGEVPENREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRVSNRTGFAVGKNVDQVEEKLLKVVPAEFKLDVHHWLI
HHHEEEECCCCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHEECHHHEEE
LHGRYTCLARKPRCGSCIIEDLCEYKEKVYPQE
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]