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The map label for this gene is gloA [H]

Identifier: 145299431

GI number: 145299431

Start: 2654795

End: 2655208

Strand: Direct

Name: gloA [H]

Synonym: ASA_2492

Alternate gene names: 145299431

Gene position: 2654795-2655208 (Clockwise)

Preceding gene: 145299430

Following gene: 145299434

Centisome position: 56.46

GC content: 57.49

Gene sequence:

>414_bases
ATGAGAATTCTGCATACCATGCTGCGTGTTGGCGATCTGCAACGCTCCATCGACTTCTACACCCGAGTGCTGGGTATGAA
ACTCTTGCGCAAGAGCGAAAACAGCGAATACAAGTACACCCTGGCCTTCGTTGGCTACGGTGACGAGAAAGACGAAGCCG
TCATCGAGCTCACCTACAACTGGGGGGTCAGCGAGTACGAACTGGGCTCGGCCTACGGCCACATTGCGCTGGAAGCGGAC
GACATCTATGCCACCTGCGATGCCCTGCGTGCCGCCGGTGCCAAGATCACCCGCGAGCCAGGTCCAGTGAAGGGCGGTAC
CACTGTCATCGCCTTCGTGGAAGATCCGGACGGTTACAAGATTGAACTGATCGCCAAGAAAGATGCAGGCAGCGGCCTGG
GTGACAGCTTCTGA

Upstream 100 bases:

>100_bases
CAGCATGCAGGCTGTACGCTATAGCCCGCAAACTTCTGCTGCGATTCCTGTATCATCCAAGACTTGTGTGAGTACAAAAA
TCTACCCGGAGAACTGAGCA

Downstream 100 bases:

>100_bases
GGCAATGCCTCAGGCAACGCCCAATAAAAAAGAGCGCTGCGGCGCTCTTTTTTCATGTCTGTGTGCAGGTTATCAGATGG
TGCCATTGCGGCCCATATTG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MRILHTMLRVGDLQRSIDFYTRVLGMKLLRKSENSEYKYTLAFVGYGDEKDEAVIELTYNWGVSEYELGSAYGHIALEAD
DIYATCDALRAAGAKITREPGPVKGGTTVIAFVEDPDGYKIELIAKKDAGSGLGDSF

Sequences:

>Translated_137_residues
MRILHTMLRVGDLQRSIDFYTRVLGMKLLRKSENSEYKYTLAFVGYGDEKDEAVIELTYNWGVSEYELGSAYGHIALEAD
DIYATCDALRAAGAKITREPGPVKGGTTVIAFVEDPDGYKIELIAKKDAGSGLGDSF
>Mature_137_residues
MRILHTMLRVGDLQRSIDFYTRVLGMKLLRKSENSEYKYTLAFVGYGDEKDEAVIELTYNWGVSEYELGSAYGHIALEAD
DIYATCDALRAAGAKITREPGPVKGGTTVIAFVEDPDGYKIELIAKKDAGSGLGDSF

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=148, Percent_Identity=38.5135135135135, Blast_Score=100, Evalue=7e-22,
Organism=Homo sapiens, GI217330598, Length=117, Percent_Identity=35.8974358974359, Blast_Score=63, Evalue=1e-10,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=77.037037037037, Blast_Score=219, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=30.8270676691729, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=42.1428571428571, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=36.3013698630137, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920404, Length=143, Percent_Identity=34.965034965035, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15094; Mature: 15094

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTMLRVGDLQRSIDFYTRVLGMKLLRKSENSEYKYTLAFVGYGDEKDEAVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGVSEYELGSAYGHIALEADDIYATCDALRAAGAKITREPGPVKGGTTVIAFVEDPDGYK
CCCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IELIAKKDAGSGLGDSF
EEEEEECCCCCCCCCCC
>Mature Secondary Structure
MRILHTMLRVGDLQRSIDFYTRVLGMKLLRKSENSEYKYTLAFVGYGDEKDEAVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGVSEYELGSAYGHIALEADDIYATCDALRAAGAKITREPGPVKGGTTVIAFVEDPDGYK
CCCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IELIAKKDAGSGLGDSF
EEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]