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The map label for this gene is rnfA [H]

Identifier: 145299424

GI number: 145299424

Start: 2647963

End: 2648544

Strand: Direct

Name: rnfA [H]

Synonym: ASA_2485

Alternate gene names: 145299424

Gene position: 2647963-2648544 (Clockwise)

Preceding gene: 145299421

Following gene: 145299425

Centisome position: 56.31

GC content: 57.22

Gene sequence:

>582_bases
ATGACTGAGTACCTGCTTTTATTGGTCAGTACCGTGCTGATTAACAACTTCGTGCTGGTGAAGTTTCTCGGCCTGTGTCC
CTTTATGGGCGTCTCCGGCAAGCTGGAGACCGCCGTCGGCATGGGGCTGGCGACCACCTTTGTGATGACGCTGGCCAGCG
CCAGTTCCTATCTGATGGAACACTATATCCTGATCCCGCTGAACATCGCCTACCTGCGCACCCTTGCCTTCATTCTTGTG
ATCGCCGTCGTAGTGCAGTTCACCGAGATGGTTATCCGCAAGAGCAGCCCGACCCTCTATCGCCTGCTGGGCATCTTCCT
GCCGCTCATCACCACCAACTGCGCCGTGCTGGGGGTGGCACTGCTGAGCATCAACGAGCGTCACAACTTCATTCAAAGCA
TCATCTACGGATTTGGCGCAGCCGCAGGCTTCTCGCTGGTACTGATACTGTTTGCCGCCATGCGTGAACGCCTGGTGGCG
GCCGACGTTCCCACTCCGTTTCGCGGTGTGTCGATTGCCATGGTCACCGCCGGCCTGATGTCGCTGGCCTTTATGGGCTT
TACCGGCCTTATCAAGATATAG

Upstream 100 bases:

>100_bases
AAATTGGCCATTTGAATAATAACCATGCGGCCTTGCGGTTGTTTAAGGAGACCTAAAATCAGATAATACCGCCGCCTTCA
AACCGGATAACCCATCTCCC

Downstream 100 bases:

>100_bases
CGCTATGACTCACATACTCTTTGCCGTTCTGGTACTGGCCCTGCTGGCCCTTGCCTTCGGAATCATTCTGGGCTTTGCCG
CCGTCAAGTTTTACGTGGAA

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTEYLLLLVSTVLINNFVLVKFLGLCPFMGVSGKLETAVGMGLATTFVMTLASASSYLMEHYILIPLNIAYLRTLAFILV
IAVVVQFTEMVIRKSSPTLYRLLGIFLPLITTNCAVLGVALLSINERHNFIQSIIYGFGAAAGFSLVLILFAAMRERLVA
ADVPTPFRGVSIAMVTAGLMSLAFMGFTGLIKI

Sequences:

>Translated_193_residues
MTEYLLLLVSTVLINNFVLVKFLGLCPFMGVSGKLETAVGMGLATTFVMTLASASSYLMEHYILIPLNIAYLRTLAFILV
IAVVVQFTEMVIRKSSPTLYRLLGIFLPLITTNCAVLGVALLSINERHNFIQSIIYGFGAAAGFSLVLILFAAMRERLVA
ADVPTPFRGVSIAMVTAGLMSLAFMGFTGLIKI
>Mature_192_residues
TEYLLLLVSTVLINNFVLVKFLGLCPFMGVSGKLETAVGMGLATTFVMTLASASSYLMEHYILIPLNIAYLRTLAFILVI
AVVVQFTEMVIRKSSPTLYRLLGIFLPLITTNCAVLGVALLSINERHNFIQSIIYGFGAAAGFSLVLILFAAMRERLVAA
DVPTPFRGVSIAMVTAGLMSLAFMGFTGLIKI

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=193, Percent_Identity=79.2746113989637, Blast_Score=311, Evalue=2e-86,
Organism=Escherichia coli, GI1787919, Length=156, Percent_Identity=33.974358974359, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20908; Mature: 20777

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYLLLLVSTVLINNFVLVKFLGLCPFMGVSGKLETAVGMGLATTFVMTLASASSYLME
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HYILIPLNIAYLRTLAFILVIAVVVQFTEMVIRKSSPTLYRLLGIFLPLITTNCAVLGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLSINERHNFIQSIIYGFGAAAGFSLVLILFAAMRERLVAADVPTPFRGVSIAMVTAGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SLAFMGFTGLIKI
HHHHHHHHHHHCC
>Mature Secondary Structure 
TEYLLLLVSTVLINNFVLVKFLGLCPFMGVSGKLETAVGMGLATTFVMTLASASSYLME
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HYILIPLNIAYLRTLAFILVIAVVVQFTEMVIRKSSPTLYRLLGIFLPLITTNCAVLGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLSINERHNFIQSIIYGFGAAAGFSLVLILFAAMRERLVAADVPTPFRGVSIAMVTAGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SLAFMGFTGLIKI
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA