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The map label for this gene is pleD [H]

Identifier: 145299391

GI number: 145299391

Start: 2605530

End: 2606777

Strand: Direct

Name: pleD [H]

Synonym: ASA_2441

Alternate gene names: 145299391

Gene position: 2605530-2606777 (Clockwise)

Preceding gene: 145299390

Following gene: 145299393

Centisome position: 55.41

GC content: 58.25

Gene sequence:

>1248_bases
ATGAAACAGAAAATATTGATTGTCGAAGATAGCATGACGATCCGTCGCACGTTGATCCAGGCCATCGCGCAGCAAACTGG
CCTGGAGATTGATGCCTTCGATACCCTGGAGGGGGCACGTCACTGCCAGGGAGACGAATACGTGGTCGCGCTAGTGGATC
TCACCCTGCCTGATGCTCCCAGCGGGGAAGCCGTCAAGGTGCTGCTGGAACGAGGCTTGCCAGTGGTGATCCTCACCGCC
GACATCAGCGAAGACAAGCGGGAGGCCTGGCTGGAAGCAGGGGTGCTGGATTATGTGATGAAAGATTCACGTCACTCCCT
GCAATACGCGGTCGGTCTGGTGCATCGCCTCTATCTCAATCAGCAGATTGAAGTGCTGGTCGTCGATGATTCACGCACCT
CCCGCCACCGCACCATGGCACAGCTACGCAAGCAGTTGTTGCTGGTCCACGAGGCGAGCCATGCCCGCGAAGCCCTGACC
ACCCTTGAGCAGAATCCGGCCATCCGGCTGGTACTGGTCGACTACTACATGCCGGAAATTGACGGCATCAGCCTGGTACG
GATGCTGCGAGAGCGCTACAGCAAGCAGCAGCTCGCCATCATAGGGATCTCGGTGTCGGACAAACGGGGCTTGTCTGCTC
GCTACCTCAAGCAAGGGGCCAACGACTTTCTCAACCAGCCGTTCGAGCCGGAAGAGTTGCAGTGTCGGGTCAGCCATAAC
CTGGAGGCGCTTGAGCAGTTCAACAGCATTCAGGAATCCGCCAACCGCGACTACCTGACCGGGCTTTATAACCGGCGCTA
CTGGTTCAACGAGGGGCAAAAGTGGTTTGAGGAGCATCAGCGCCAGCAGACGCCGCTCGCGCTGTGTGTCCTCGATGTGG
ATCACTTCAAGCAGGTCAACGATCACTGGGGTCATGCCATCGGCGATCAACTGCTCTGCCACCTCACCCGGTTGCTGACC
CAGTTCTTCCCCGATGCCATGATTGCCCGCTTTGGGGGGGAGGAGTTTGCCCTTATGGTGCCCCATTGCACAGTGCCAGT
GCTGCTCAAACGGCTGGAGGGACTGCGCCAACAACTGCGTCAGCAACCCTTGTCACGGGAGACGCCACTGTTCGTTCGCG
CCAGTTTCGGGGTGATCAATGTCGGGCGAGTCTCCATGGACGAGGCACTGAGTGAAGCGGATCGCCTGCTCTATCAGGCA
AAAAACACAGGTCGCGACAAGATAGTGTCTCAGGGCTCATCTGCATGA

Upstream 100 bases:

>100_bases
CATGGCCGCTCTCGATGCCGAGCGATATCTGGACGCGCAATAAGCGCAAGGGATATAAAAGGCATGCCGTGAGGCATGCC
TTTTTACGTTAAGGAGCCGG

Downstream 100 bases:

>100_bases
GCTAGATGAGTTCCGTGTAGTACCGCCCGACTGGCTCCTGTCAATGGAGACCGTCGGGTTGCTCCCCTTCCCTCTTCCCT
GCTTCGCTCGATGCGCCCTG

Product: two-component response transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKQKILIVEDSMTIRRTLIQAIAQQTGLEIDAFDTLEGARHCQGDEYVVALVDLTLPDAPSGEAVKVLLERGLPVVILTA
DISEDKREAWLEAGVLDYVMKDSRHSLQYAVGLVHRLYLNQQIEVLVVDDSRTSRHRTMAQLRKQLLLVHEASHAREALT
TLEQNPAIRLVLVDYYMPEIDGISLVRMLRERYSKQQLAIIGISVSDKRGLSARYLKQGANDFLNQPFEPEELQCRVSHN
LEALEQFNSIQESANRDYLTGLYNRRYWFNEGQKWFEEHQRQQTPLALCVLDVDHFKQVNDHWGHAIGDQLLCHLTRLLT
QFFPDAMIARFGGEEFALMVPHCTVPVLLKRLEGLRQQLRQQPLSRETPLFVRASFGVINVGRVSMDEALSEADRLLYQA
KNTGRDKIVSQGSSA

Sequences:

>Translated_415_residues
MKQKILIVEDSMTIRRTLIQAIAQQTGLEIDAFDTLEGARHCQGDEYVVALVDLTLPDAPSGEAVKVLLERGLPVVILTA
DISEDKREAWLEAGVLDYVMKDSRHSLQYAVGLVHRLYLNQQIEVLVVDDSRTSRHRTMAQLRKQLLLVHEASHAREALT
TLEQNPAIRLVLVDYYMPEIDGISLVRMLRERYSKQQLAIIGISVSDKRGLSARYLKQGANDFLNQPFEPEELQCRVSHN
LEALEQFNSIQESANRDYLTGLYNRRYWFNEGQKWFEEHQRQQTPLALCVLDVDHFKQVNDHWGHAIGDQLLCHLTRLLT
QFFPDAMIARFGGEEFALMVPHCTVPVLLKRLEGLRQQLRQQPLSRETPLFVRASFGVINVGRVSMDEALSEADRLLYQA
KNTGRDKIVSQGSSA
>Mature_415_residues
MKQKILIVEDSMTIRRTLIQAIAQQTGLEIDAFDTLEGARHCQGDEYVVALVDLTLPDAPSGEAVKVLLERGLPVVILTA
DISEDKREAWLEAGVLDYVMKDSRHSLQYAVGLVHRLYLNQQIEVLVVDDSRTSRHRTMAQLRKQLLLVHEASHAREALT
TLEQNPAIRLVLVDYYMPEIDGISLVRMLRERYSKQQLAIIGISVSDKRGLSARYLKQGANDFLNQPFEPEELQCRVSHN
LEALEQFNSIQESANRDYLTGLYNRRYWFNEGQKWFEEHQRQQTPLALCVLDVDHFKQVNDHWGHAIGDQLLCHLTRLLT
QFFPDAMIARFGGEEFALMVPHCTVPVLLKRLEGLRQQLRQQPLSRETPLFVRASFGVINVGRVSMDEALSEADRLLYQA
KNTGRDKIVSQGSSA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=35.5421686746988, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=35.7575757575758, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=33.1288343558282, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=31.3513513513514, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=32.0441988950276, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=29.8136645962733, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=30.8571428571429, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=167, Percent_Identity=31.1377245508982, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787816, Length=169, Percent_Identity=31.9526627218935, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47370; Mature: 47370

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKILIVEDSMTIRRTLIQAIAQQTGLEIDAFDTLEGARHCQGDEYVVALVDLTLPDAP
CCCEEEEEECCHHHHHHHHHHHHHHHCCEEECHHHHHHHHCCCCCCEEEEEEEEECCCCC
SGEAVKVLLERGLPVVILTADISEDKREAWLEAGVLDYVMKDSRHSLQYAVGLVHRLYLN
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
QQIEVLVVDDSRTSRHRTMAQLRKQLLLVHEASHAREALTTLEQNPAIRLVLVDYYMPEI
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
DGISLVRMLRERYSKQQLAIIGISVSDKRGLSARYLKQGANDFLNQPFEPEELQCRVSHN
CHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHCHHHHHCCCCCCHHHHEEHHCC
LEALEQFNSIQESANRDYLTGLYNRRYWFNEGQKWFEEHQRQQTPLALCVLDVDHFKQVN
HHHHHHHHHHHHHCCCCHHHHHHHCCEECCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHH
DHWGHAIGDQLLCHLTRLLTQFFPDAMIARFGGEEFALMVPHCTVPVLLKRLEGLRQQLR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
QQPLSRETPLFVRASFGVINVGRVSMDEALSEADRLLYQAKNTGRDKIVSQGSSA
HCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MKQKILIVEDSMTIRRTLIQAIAQQTGLEIDAFDTLEGARHCQGDEYVVALVDLTLPDAP
CCCEEEEEECCHHHHHHHHHHHHHHHCCEEECHHHHHHHHCCCCCCEEEEEEEEECCCCC
SGEAVKVLLERGLPVVILTADISEDKREAWLEAGVLDYVMKDSRHSLQYAVGLVHRLYLN
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
QQIEVLVVDDSRTSRHRTMAQLRKQLLLVHEASHAREALTTLEQNPAIRLVLVDYYMPEI
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
DGISLVRMLRERYSKQQLAIIGISVSDKRGLSARYLKQGANDFLNQPFEPEELQCRVSHN
CHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHCHHHHHCCCCCCHHHHEEHHCC
LEALEQFNSIQESANRDYLTGLYNRRYWFNEGQKWFEEHQRQQTPLALCVLDVDHFKQVN
HHHHHHHHHHHHHCCCCHHHHHHHCCEECCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHH
DHWGHAIGDQLLCHLTRLLTQFFPDAMIARFGGEEFALMVPHCTVPVLLKRLEGLRQQLR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
QQPLSRETPLFVRASFGVINVGRVSMDEALSEADRLLYQAKNTGRDKIVSQGSSA
HCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA