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The map label for this gene is trpB1 [H]

Identifier: 145297023

GI number: 145297023

Start: 3241055

End: 3242308

Strand: Direct

Name: trpB1 [H]

Synonym: cgR_2920

Alternate gene names: 145297023

Gene position: 3241055-3242308 (Clockwise)

Preceding gene: 145297022

Following gene: 145297024

Centisome position: 97.79

GC content: 61.88

Gene sequence:

>1254_bases
ATGACTGAAAAAGAAAACTTGGGTGGCTCCACGCTGCTGCCTGCATACTTCGGTGAATTCGGCGGCCAGTTCGTCGCGGA
ATCCCTCCTGCCTGCTCTCGACCAGCTGGAGAAGGCCTTCGTTGACGCGACCAACAGCCCAGAGTTCCGCGAAGAACTCG
GCGGCTACCTCCGCGATTACCTCGGCCGCCCAACCCCGCTGACCGAATGCTCCAACCTGCCACTCTCAGGCGAAGGCAAA
GGCTTTGCGCGGATCTTCCTCAAGCGCGAAGACCTCGTCCACGGCGGTGCACACAAAACTAACCAGGTGATCGGCCAGGT
GCTGCTTGCCAAGCGCATGGGCAAAACCCGCATCATCGCAGAGACCGGCGCAGGCCAGCACGGCACCGCCACCGCTCTCG
CATGTGCGCTCATGGGCCTCGAGTGCGTTGTCTACATGGGCGCCAAGGACGTTGCCCGCCAGCAGCCCAACGTCTACCGC
ATGCAGCTGCACGGCGCGAAGGTCATCCCCGTGGAATCTGGTTCCGGCACCCTGAAGGACGCCGTGAATGAAGCGCTGCG
CGATTGGACCGCAACCTTCCACGAGTCCCACTACCTTCTCGGCACCGCCGCCGGCCCGCATCCATTCCCAACCATCGTGC
GTGAATTCCACAAGGTGATCTCTGAGGAAGCCAAGGCACAGATGCTAGAGCGCACCGGCAAGCTTCCCGACGTTGTGGTC
GCCTGTGTCGGTGGTGGCTCCAACGCCATCGGCATGTTCGCAGACTTCATTGACGATGAAGGCGTAGAGCTCGTCGGCGC
TGAGCCAGCCGGTGAAGGCCTCGACTCCGGCAAGCACGGCGCAACCATCACCAACGGTCAGATCGGCATCCTACACGGCA
CCCGTTCCTACCTGATGCGCAACTCCGACGGCCAAGTGGAAGAGTCCTACTCCATCTCCGCCGGACTTGATTACCCAGGC
GTCGGCCCACAGCACGCACACCTGCACGCCACCGGCCGCGCCACCTACGTTGGTATCACCGACGCCGAAGCCCTCCAAGC
ATTCCAGTATCTCGCCCGCTACGAAGGCATCATCCCCGCACTGGAATCCTCACACGCATTCGCCTACGCACTCAAGCGCG
CCAAGACCGCCGAAGAGGAAGGCCAGAACTTAACCATCCTCGTCTCCCTATCCGGCCGTGGCGACAAGGACGTTGACCAC
GTGCGCCGCACCCTCGAAGAAAATCCAGAACTGATCCTGAAGGACAACCGATGA

Upstream 100 bases:

>100_bases
GCCGGTGCAGGCACGTGGGCTGGGGCGAAAGACGCCGGCGCGCTGCTGAAAATTTTCGCAACCATCTCCACATTCCACTA
CTAAAGGTTTAAATAGGATC

Downstream 100 bases:

>100_bases
GCCGTTACGACGATCTTTTTGCACGCCTCGACACGGCAGGGGAGGGCGCCTTTGTTCCCTTCATCATGCTGAGCGACCCT
TCACCAGAGGAGGCTTTCCA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTEKENLGGSTLLPAYFGEFGGQFVAESLLPALDQLEKAFVDATNSPEFREELGGYLRDYLGRPTPLTECSNLPLSGEGK
GFARIFLKREDLVHGGAHKTNQVIGQVLLAKRMGKTRIIAETGAGQHGTATALACALMGLECVVYMGAKDVARQQPNVYR
MQLHGAKVIPVESGSGTLKDAVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHKVISEEAKAQMLERTGKLPDVVV
ACVGGGSNAIGMFADFIDDEGVELVGAEPAGEGLDSGKHGATITNGQIGILHGTRSYLMRNSDGQVEESYSISAGLDYPG
VGPQHAHLHATGRATYVGITDAEALQAFQYLARYEGIIPALESSHAFAYALKRAKTAEEEGQNLTILVSLSGRGDKDVDH
VRRTLEENPELILKDNR

Sequences:

>Translated_417_residues
MTEKENLGGSTLLPAYFGEFGGQFVAESLLPALDQLEKAFVDATNSPEFREELGGYLRDYLGRPTPLTECSNLPLSGEGK
GFARIFLKREDLVHGGAHKTNQVIGQVLLAKRMGKTRIIAETGAGQHGTATALACALMGLECVVYMGAKDVARQQPNVYR
MQLHGAKVIPVESGSGTLKDAVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHKVISEEAKAQMLERTGKLPDVVV
ACVGGGSNAIGMFADFIDDEGVELVGAEPAGEGLDSGKHGATITNGQIGILHGTRSYLMRNSDGQVEESYSISAGLDYPG
VGPQHAHLHATGRATYVGITDAEALQAFQYLARYEGIIPALESSHAFAYALKRAKTAEEEGQNLTILVSLSGRGDKDVDH
VRRTLEENPELILKDNR
>Mature_416_residues
TEKENLGGSTLLPAYFGEFGGQFVAESLLPALDQLEKAFVDATNSPEFREELGGYLRDYLGRPTPLTECSNLPLSGEGKG
FARIFLKREDLVHGGAHKTNQVIGQVLLAKRMGKTRIIAETGAGQHGTATALACALMGLECVVYMGAKDVARQQPNVYRM
QLHGAKVIPVESGSGTLKDAVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHKVISEEAKAQMLERTGKLPDVVVA
CVGGGSNAIGMFADFIDDEGVELVGAEPAGEGLDSGKHGATITNGQIGILHGTRSYLMRNSDGQVEESYSISAGLDYPGV
GPQHAHLHATGRATYVGITDAEALQAFQYLARYEGIIPALESSHAFAYALKRAKTAEEEGQNLTILVSLSGRGDKDVDHV
RRTLEENPELILKDNR

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=402, Percent_Identity=62.1890547263682, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6321412, Length=393, Percent_Identity=51.6539440203562, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 44813; Mature: 44682

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKENLGGSTLLPAYFGEFGGQFVAESLLPALDQLEKAFVDATNSPEFREELGGYLRDY
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGRPTPLTECSNLPLSGEGKGFARIFLKREDLVHGGAHKTNQVIGQVLLAKRMGKTRIIA
CCCCCCCHHHCCCCCCCCCCCEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEE
ETGAGQHGTATALACALMGLECVVYMGAKDVARQQPNVYRMQLHGAKVIPVESGSGTLKD
ECCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHCCCCEEEEEECCCEEEEEECCCCHHHH
AVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHKVISEEAKAQMLERTGKLPDVVV
HHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ACVGGGSNAIGMFADFIDDEGVELVGAEPAGEGLDSGKHGATITNGQIGILHGTRSYLMR
EEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEECHHHHHEE
NSDGQVEESYSISAGLDYPGVGPQHAHLHATGRATYVGITDAEALQAFQYLARYEGIIPA
CCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCC
LESSHAFAYALKRAKTAEEEGQNLTILVSLSGRGDKDVDHVRRTLEENPELILKDNR
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
TEKENLGGSTLLPAYFGEFGGQFVAESLLPALDQLEKAFVDATNSPEFREELGGYLRDY
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGRPTPLTECSNLPLSGEGKGFARIFLKREDLVHGGAHKTNQVIGQVLLAKRMGKTRIIA
CCCCCCCHHHCCCCCCCCCCCEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEE
ETGAGQHGTATALACALMGLECVVYMGAKDVARQQPNVYRMQLHGAKVIPVESGSGTLKD
ECCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHCCCCEEEEEECCCEEEEEECCCCHHHH
AVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHKVISEEAKAQMLERTGKLPDVVV
HHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ACVGGGSNAIGMFADFIDDEGVELVGAEPAGEGLDSGKHGATITNGQIGILHGTRSYLMR
EEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEECHHHHHEE
NSDGQVEESYSISAGLDYPGVGPQHAHLHATGRATYVGITDAEALQAFQYLARYEGIIPA
CCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCC
LESSHAFAYALKRAKTAEEEGQNLTILVSLSGRGDKDVDHVRRTLEENPELILKDNR
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]