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The map label for this gene is trpA

Identifier: 145297024

GI number: 145297024

Start: 3242305

End: 3243147

Strand: Direct

Name: trpA

Synonym: cgR_2921

Alternate gene names: 145297024

Gene position: 3242305-3243147 (Clockwise)

Preceding gene: 145297023

Following gene: 145297027

Centisome position: 97.83

GC content: 59.19

Gene sequence:

>843_bases
ATGAGCCGTTACGACGATCTTTTTGCACGCCTCGACACGGCAGGGGAGGGCGCCTTTGTTCCCTTCATCATGCTGAGCGA
CCCTTCACCAGAGGAGGCTTTCCAGATCATCTCCACAGCAATCGAAGCTGGCGCAGATGCACTGGAACTTGGCGTACCTT
TCTCCGACCCAGTTGCCGATGGCCCCACCGTCGCGGAATCCCACCTCCGCGCACTCGACGGCGGCGCCACCGTAGACAGC
GCACTCGAGCAGATCAAGCGCGTGCGCGCAGCCTACCCAGAGGTTCCCATCGGAATGCTCATCTACGGCAACGTTCCTTT
CACCCGTGGCTTGGATCGCTTCTACCAAGAGTTCGCTGAAGCTGGCGCAGACTCCATCCTCCTGCCAGACGTCCCAGTCC
GCGAAGGCGCACCGTTTTCTGCAGCAGCTGCAGCAGCCGGAATTGATCCCATTTACATCGCTCCGGCCAACGCCAGCGAG
AAAACCCTCGAGGGTGTCTCCGCCGCATCAAAGGGCTACATCTACGCCATCTCCCGCGACGGCGTCACCGGCACCGAACG
TGAATCATCCACCGACGGCCTGTCCGCAGTGGTGGACAATATCAAGAAATTTGATGGCGCACCCATCTTCTTGGGCTTCG
GCATCTCATCCCCTCAGCACGTGGCAGACGCGATTGCAGCGGGTGCTTCCGGTGCGATCACGGGTTCCGCGATCACCAAG
ATCATTGCTTCCCACTGCGAAGGTGAGCACCCGAACCCGTCCACCATTCGAGATATGGACGGTTTGAAGAAGGATCTCAC
TGAGTTCATCTCTGCGATGAAGGCAGCGACCAAGAAGGTTTAG

Upstream 100 bases:

>100_bases
TAACCATCCTCGTCTCCCTATCCGGCCGTGGCGACAAGGACGTTGACCACGTGCGCCGCACCCTCGAAGAAAATCCAGAA
CTGATCCTGAAGGACAACCG

Downstream 100 bases:

>100_bases
GCCTTTAAATGTGGCAATGTTTCACGTGAAACATTCTGAGAGAATGTAGAAACATCAAAGAAGCCGCCTCCTAGCTCTCG
GGCTGGGAGGCGGCTTCTTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSRYDDLFARLDTAGEGAFVPFIMLSDPSPEEAFQIISTAIEAGADALELGVPFSDPVADGPTVAESHLRALDGGATVDS
ALEQIKRVRAAYPEVPIGMLIYGNVPFTRGLDRFYQEFAEAGADSILLPDVPVREGAPFSAAAAAAGIDPIYIAPANASE
KTLEGVSAASKGYIYAISRDGVTGTERESSTDGLSAVVDNIKKFDGAPIFLGFGISSPQHVADAIAAGASGAITGSAITK
IIASHCEGEHPNPSTIRDMDGLKKDLTEFISAMKAATKKV

Sequences:

>Translated_280_residues
MSRYDDLFARLDTAGEGAFVPFIMLSDPSPEEAFQIISTAIEAGADALELGVPFSDPVADGPTVAESHLRALDGGATVDS
ALEQIKRVRAAYPEVPIGMLIYGNVPFTRGLDRFYQEFAEAGADSILLPDVPVREGAPFSAAAAAAGIDPIYIAPANASE
KTLEGVSAASKGYIYAISRDGVTGTERESSTDGLSAVVDNIKKFDGAPIFLGFGISSPQHVADAIAAGASGAITGSAITK
IIASHCEGEHPNPSTIRDMDGLKKDLTEFISAMKAATKKV
>Mature_279_residues
SRYDDLFARLDTAGEGAFVPFIMLSDPSPEEAFQIISTAIEAGADALELGVPFSDPVADGPTVAESHLRALDGGATVDSA
LEQIKRVRAAYPEVPIGMLIYGNVPFTRGLDRFYQEFAEAGADSILLPDVPVREGAPFSAAAAAAGIDPIYIAPANASEK
TLEGVSAASKGYIYAISRDGVTGTERESSTDGLSAVVDNIKKFDGAPIFLGFGISSPQHVADAIAAGASGAITGSAITKI
IASHCEGEHPNPSTIRDMDGLKKDLTEFISAMKAATKKV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=278, Percent_Identity=46.0431654676259, Blast_Score=236, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321412, Length=243, Percent_Identity=30.0411522633745, Blast_Score=123, Evalue=4e-29,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_CORGB (A4QI77)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139845.1
- ProteinModelPortal:   A4QI77
- SMR:   A4QI77
- STRING:   A4QI77
- GeneID:   4992867
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2921
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29109; Mature: 28978

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYDDLFARLDTAGEGAFVPFIMLSDPSPEEAFQIISTAIEAGADALELGVPFSDPVAD
CCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTVAESHLRALDGGATVDSALEQIKRVRAAYPEVPIGMLIYGNVPFTRGLDRFYQEFAE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
AGADSILLPDVPVREGAPFSAAAAAAGIDPIYIAPANASEKTLEGVSAASKGYIYAISRD
CCCCCEECCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECC
GVTGTERESSTDGLSAVVDNIKKFDGAPIFLGFGISSPQHVADAIAAGASGAITGSAITK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHH
IIASHCEGEHPNPSTIRDMDGLKKDLTEFISAMKAATKKV
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRYDDLFARLDTAGEGAFVPFIMLSDPSPEEAFQIISTAIEAGADALELGVPFSDPVAD
CHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTVAESHLRALDGGATVDSALEQIKRVRAAYPEVPIGMLIYGNVPFTRGLDRFYQEFAE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
AGADSILLPDVPVREGAPFSAAAAAAGIDPIYIAPANASEKTLEGVSAASKGYIYAISRD
CCCCCEECCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECC
GVTGTERESSTDGLSAVVDNIKKFDGAPIFLGFGISSPQHVADAIAAGASGAITGSAITK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHH
IIASHCEGEHPNPSTIRDMDGLKKDLTEFISAMKAATKKV
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA