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The map label for this gene is pykF [C]

Identifier: 145296911

GI number: 145296911

Start: 3122339

End: 3124198

Strand: Reverse

Name: pykF [C]

Synonym: cgR_2811

Alternate gene names: 145296911

Gene position: 3124198-3122339 (Counterclockwise)

Preceding gene: 145296912

Following gene: 145296905

Centisome position: 94.27

GC content: 58.66

Gene sequence:

>1860_bases
ATGAATGAGTTTGACCAGGACATTCTCCAGGAGATCAAGACTGAACTCGACGAGTTAATTCTAGAACTTGATGAGGTGAC
ACAAACTCACAGCGAGGCCATCGGGCAGGTCTCCCCAACCCATTACGTTGGTGCCCGCAACCTCATGCATTACGCGCATC
TTCGCACCAAAGACCTCCGTGGCCTGCAGCAACGCCTCTCCTCTGTGGGAGCTACCCGCTTGACTACCACCGAACCAGCA
GTGCAGGCCCGCCTCAAGGCCGCCCGCAATGTTATCGGAGCTTTCGCAGGTGAAGGCCCACTTTATCCACCCTCAGATGT
CGTCGATGCCTTCGAAGATGCCGATGAGATTCTCGACGAGCACGCCGAAATTCTCCTTGGCGAACCCCTACCGGATACTC
CATCCTGCATCATGGTCACCCTGCCCACCGAAGCCGCCACCGACATCGAACTTGTCCGTGGCTTCGCCAAAAGCGGCATG
GATCTAGCTCGCATCAACTGTGCCCACGACGATGAAACCGTCTGGAAGCAGATGATCGACAACGTCCACACCGTTGCAGA
AGAAGTTGGCCGGGAAATCCGCGTCAGCATGGACCTCGCCGGACCAAAAGTACGCACCGGCGAAATCGCCCCAGGCGCAG
AAGTAGGTCGCGCACGAGTAACCCGCGACAAAACCGGAAAAGTGCTCACTCCCGCAAAACTGTGGATCACCGCCCACGGC
TCCGAACCAGTCCCAGCCCCCGAAAGCCTGCCCGGTCGCCCCGCTCTGCCGATTGAAGTCACCCCAGAATGGTTCGACAA
ACTAGAAATCGGCAGCGTCATCAACGTCCCAGACACCCGCGGATCCCGCCGAGCATTCACCGTGACCAGGGTTTTTGATG
GCGCGGTCCTCGCCGAAGGCCCACAAAAAGCCTACATCTCCAACGGCACCCTCCTGGAACACAACTACGACCGCTCCCGG
GTCTACGGCATCCCCGCCGTAGTTCAGCGCATCAACCTCAAAGTCGGCGACCGCCTCATCCTTACCGACGAAGAACTCAC
CTACGATCCATCCCTCGGATCCGGCCGCACACCACGCATCAGCTGTACCCTTCCACAAGCAGTCGATGCAATTAAGGTCG
GCCACCGCGTGCTTTTCGACGACGGAGCCATCGCCGCAGTCTGCATCGACAAGGCCTCCACCACCGACGGCTTCAACGAC
GTGGAATTGGAAGTCACCCGCGCCCGTCCACAAGGTGTAAACCTGGCCGCATACAAGGGAATCAACCTCCCGGACTCCGA
GCTTCCACTCCCAAGCCTCACCGAAGAAGACCTCCAACACCTGCGCTTTGTGGTCCAATACGCCGACATCGCAGCCATCT
CCTTCATCCGAAACGTCGCTGATGTGGAATACCTCCTCCAAGCACTCGCCGACATCGGAGACCCAGCAGCTGTCGAACGT
CTCGGACTCGTCCTCAAAATCGAGACCATCCCAGGCTACGAAGGCCTCGCCCAAATCCTCCTGACCGGCATGCGCCACGA
AAACTTCGGTATCATGATCGCCCGCGGAGACCTCGCCGTCGAACTCGGCTTCGACCGCATGGCAGAAGTCCCCCAACTGA
TCATGGCCCTTGCCGAAGCCGCCCACGTCCCAACCATCCTGGCCACCCAAGTCCTGGAAAACATGGCCAAAAACGGACTC
CCATCCCGCGCAGAAATCACCGACGCAGCAATGGCACTTCGCGCCGAATGCGTCATGCTGAACAAGGGACCACACATCAA
CGACGCCATCAAGGTCCTCACCAAAATGAGCCGCAAACTTGGTGCATCCCAACGAAAGAGCAGGCTGCTGCTGCGCAAGG
TGAAGAGCTGGGAAGAGTAA

Upstream 100 bases:

>100_bases
AAGTGTTTCATTGGAACACTTGCGCTGCCAACTTTTTGGTTTACGGGCAAAATGAAACTGTTGGATGGAATTTAGAGTGT
TTGTAGCTTAAGGAGCTCAA

Downstream 100 bases:

>100_bases
CTCACAAAGGCGATTGGCGTTAACTTCGAGACCTTAACAGCGATTTCACACCAGCGGTTTCTCACCGACGGTTTCGCGCG
CTCAATTTCTTAAGGGCGAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLRGLQQRLSSVGATRLTTTEPA
VQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDEHAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGM
DLARINCAHDDETVWKQMIDNVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDKTGKVLTPAKLWITAHG
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEGPQKAYISNGTLLEHNYDRSR
VYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRISCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKASTTDGFND
VELEVTRARPQGVNLAAYKGINLPDSELPLPSLTEEDLQHLRFVVQYADIAAISFIRNVADVEYLLQALADIGDPAAVER
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEAAHVPTILATQVLENMAKNGL
PSRAEITDAAMALRAECVMLNKGPHINDAIKVLTKMSRKLGASQRKSRLLLRKVKSWEE

Sequences:

>Translated_619_residues
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLRGLQQRLSSVGATRLTTTEPA
VQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDEHAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGM
DLARINCAHDDETVWKQMIDNVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDKTGKVLTPAKLWITAHG
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEGPQKAYISNGTLLEHNYDRSR
VYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRISCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKASTTDGFND
VELEVTRARPQGVNLAAYKGINLPDSELPLPSLTEEDLQHLRFVVQYADIAAISFIRNVADVEYLLQALADIGDPAAVER
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEAAHVPTILATQVLENMAKNGL
PSRAEITDAAMALRAECVMLNKGPHINDAIKVLTKMSRKLGASQRKSRLLLRKVKSWEE
>Mature_619_residues
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLRGLQQRLSSVGATRLTTTEPA
VQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDEHAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGM
DLARINCAHDDETVWKQMIDNVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDKTGKVLTPAKLWITAHG
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEGPQKAYISNGTLLEHNYDRSR
VYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRISCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKASTTDGFND
VELEVTRARPQGVNLAAYKGINLPDSELPLPSLTEEDLQHLRFVVQYADIAAISFIRNVADVEYLLQALADIGDPAAVER
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEAAHVPTILATQVLENMAKNGL
PSRAEITDAAMALRAECVMLNKGPHINDAIKVLTKMSRKLGASQRKSRLLLRKVKSWEE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=273, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI32967597, Length=273, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI33286422, Length=253, Percent_Identity=30.8300395256917, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33286420, Length=253, Percent_Identity=30.8300395256917, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33286418, Length=240, Percent_Identity=31.6666666666667, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI310128732, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI310128730, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1787965, Length=276, Percent_Identity=30.0724637681159, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788160, Length=258, Percent_Identity=27.906976744186, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17506831, Length=273, Percent_Identity=30.4029304029304, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17506829, Length=273, Percent_Identity=30.4029304029304, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71984406, Length=273, Percent_Identity=30.4029304029304, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71984413, Length=273, Percent_Identity=30.4029304029304, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17544584, Length=243, Percent_Identity=30.0411522633745, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319279, Length=214, Percent_Identity=32.2429906542056, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324923, Length=195, Percent_Identity=33.8461538461538, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571814, Length=256, Percent_Identity=33.984375, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648964, Length=256, Percent_Identity=33.984375, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648966, Length=280, Percent_Identity=33.5714285714286, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581235, Length=252, Percent_Identity=23.8095238095238, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 67573; Mature: 67573

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLQQRLSSVGATRLTTTEPAVQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDE
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
HAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGMDLARINCAHDDETVWKQMID
HHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
NVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDKTGKVLTPAKLWITAHG
HHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCHHCCHHCCCCCCCCEECCEEEEEEECC
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEG
CCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCEECCCCCCCCCEEEEEEEECCCCCCCCC
PQKAYISNGTLLEHNYDRSRVYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRI
CCCEEECCCEEEECCCCCHHEECHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCEE
SCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKASTTDGFNDVELEVTRARPQGVNLAAYKG
EEECHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEECC
INLPDSELPLPSLTEEDLQHLRFVVQYADIAAISFIRNVADVEYLLQALADIGDPAAVER
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEA
CCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHH
AHVPTILATQVLENMAKNGLPSRAEITDAAMALRAECVMLNKGPHINDAIKVLTKMSRKL
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
GASQRKSRLLLRKVKSWEE
CCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLQQRLSSVGATRLTTTEPAVQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDE
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
HAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGMDLARINCAHDDETVWKQMID
HHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
NVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDKTGKVLTPAKLWITAHG
HHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCHHCCHHCCCCCCCCEECCEEEEEEECC
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEG
CCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCEECCCCCCCCCEEEEEEEECCCCCCCCC
PQKAYISNGTLLEHNYDRSRVYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRI
CCCEEECCCEEEECCCCCHHEECHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCEE
SCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKASTTDGFNDVELEVTRARPQGVNLAAYKG
EEECHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEECC
INLPDSELPLPSLTEEDLQHLRFVVQYADIAAISFIRNVADVEYLLQALADIGDPAAVER
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEA
CCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHH
AHVPTILATQVLENMAKNGLPSRAEITDAAMALRAECVMLNKGPHINDAIKVLTKMSRKL
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
GASQRKSRLLLRKVKSWEE
CCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]