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The map label for this gene is ldh

Identifier: 145296912

GI number: 145296912

Start: 3124324

End: 3125268

Strand: Reverse

Name: ldh

Synonym: cgR_2812

Alternate gene names: 145296912

Gene position: 3125268-3124324 (Counterclockwise)

Preceding gene: 145296921

Following gene: 145296911

Centisome position: 94.3

GC content: 55.24

Gene sequence:

>945_bases
ATGAAAGAAACCGTCGGCAATAAGATTGTCCTTATTGGCGCAGGAGATGTTGGAGTTGCATACGCATACGCACTGATCAA
CCAGGGCATGGCAGATCACCTTGCGATCATCGACATCGATGAAAAGAAACTCGAAGGCAACGTCATGGACTTAAACCATG
GTGTTGTGTGGGCCGATTCCCGCACCCGCGTCACCAAGGGCACCTACGCTGACTGCGAGGACGCAGCCATGGTTGTCATT
TGTGCCGGCGCAGCCCAAAAGCCAGGCGAAACTCGCCTCCAGCTGGTGGACAAAAACGTCAAGATTATGAAGTCCATCGT
TGGCGATGTCATGGCCAGCGGATTCGACGGCATCTTCCTCGTAGCCTCCAACCCAGTGGATATCCTCACCTACGCAGTGT
GGAAATTCTCCGGCCTGGAATGGAACCGCGTGATCGGCTCCGGAACTGTCCTGGACTCCGCTAGATTCCGCTACATGCTC
GGCGAACTCTATGAAGTGGCACCAAGCTCCGTCCACGCCTACATCATCGGCGAACACGGCGACACTGAACTTCCAGTCCT
GTCCTCCGCGACCATCGCAGGCGTATCGCTTAGCCGCATGCTAGACAAAGACCCAGAGCTTGAGGGCCGTCTAGAGAAAA
TTTTCGAAGACACCCGCGACGCCGCCTATCACATCATCGACGCCAAGGGCTCCACTTCCTACGGCATCGGCATGGGTCTT
GCTCGCATCACCCGCGCAATCCTACAAAACCAAGACGTTGCAGTCCCAGTCTCTGCACTGCTCCACGGTGAATACGGTGA
GGAAGACATCTACATCGGCACCCCAGCAGTAGTAAACCGCCGAGGCATCCGCCGCGTTGTCGAACTAGAAATCACGGACC
ATGAGATGGAACGCTTCAAGCATTCCGCAAATACCCTGCGCGAAATTCAGAAGCAGTTCTTCTGA

Upstream 100 bases:

>100_bases
ATTAAAGGTGTAACAAAGGAATCCGGGCACAAGCTCTTGCTGATTTTCTGAGCTGCTTTGTGGGTTGTCCGGTTAGGGAA
ATCAGGAAGTGGGATCGAAA

Downstream 100 bases:

>100_bases
ATCTTTGGTGTCTAGTTGGCGGCGCAAGTGTTTCATTGGAACACTTGCGCTGCCAACTTTTTGGTTTACGGGCAAAATGA
AACTGTTGGATGGAATTTAG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKETVGNKIVLIGAGDVGVAYAYALINQGMADHLAIIDIDEKKLEGNVMDLNHGVVWADSRTRVTKGTYADCEDAAMVVI
CAGAAQKPGETRLQLVDKNVKIMKSIVGDVMASGFDGIFLVASNPVDILTYAVWKFSGLEWNRVIGSGTVLDSARFRYML
GELYEVAPSSVHAYIIGEHGDTELPVLSSATIAGVSLSRMLDKDPELEGRLEKIFEDTRDAAYHIIDAKGSTSYGIGMGL
ARITRAILQNQDVAVPVSALLHGEYGEEDIYIGTPAVVNRRGIRRVVELEITDHEMERFKHSANTLREIQKQFF

Sequences:

>Translated_314_residues
MKETVGNKIVLIGAGDVGVAYAYALINQGMADHLAIIDIDEKKLEGNVMDLNHGVVWADSRTRVTKGTYADCEDAAMVVI
CAGAAQKPGETRLQLVDKNVKIMKSIVGDVMASGFDGIFLVASNPVDILTYAVWKFSGLEWNRVIGSGTVLDSARFRYML
GELYEVAPSSVHAYIIGEHGDTELPVLSSATIAGVSLSRMLDKDPELEGRLEKIFEDTRDAAYHIIDAKGSTSYGIGMGL
ARITRAILQNQDVAVPVSALLHGEYGEEDIYIGTPAVVNRRGIRRVVELEITDHEMERFKHSANTLREIQKQFF
>Mature_314_residues
MKETVGNKIVLIGAGDVGVAYAYALINQGMADHLAIIDIDEKKLEGNVMDLNHGVVWADSRTRVTKGTYADCEDAAMVVI
CAGAAQKPGETRLQLVDKNVKIMKSIVGDVMASGFDGIFLVASNPVDILTYAVWKFSGLEWNRVIGSGTVLDSARFRYML
GELYEVAPSSVHAYIIGEHGDTELPVLSSATIAGVSLSRMLDKDPELEGRLEKIFEDTRDAAYHIIDAKGSTSYGIGMGL
ARITRAILQNQDVAVPVSALLHGEYGEEDIYIGTPAVVNRRGIRRVVELEITDHEMERFKHSANTLREIQKQFF

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI260099723, Length=318, Percent_Identity=40.8805031446541, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI5031857, Length=318, Percent_Identity=40.8805031446541, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI9257228, Length=314, Percent_Identity=42.9936305732484, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI4504973, Length=314, Percent_Identity=42.9936305732484, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI291575128, Length=314, Percent_Identity=39.4904458598726, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI4557032, Length=314, Percent_Identity=39.4904458598726, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI47059044, Length=313, Percent_Identity=41.5335463258786, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI221136809, Length=313, Percent_Identity=41.5335463258786, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI15082234, Length=313, Percent_Identity=39.297124600639, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI260099725, Length=230, Percent_Identity=43.4782608695652, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI260099727, Length=210, Percent_Identity=45.7142857142857, Blast_Score=187, Evalue=7e-48,
Organism=Homo sapiens, GI207028494, Length=216, Percent_Identity=40.2777777777778, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI103472011, Length=313, Percent_Identity=26.517571884984, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI103472015, Length=175, Percent_Identity=32, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI21735621, Length=291, Percent_Identity=26.4604810996564, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789632, Length=289, Percent_Identity=26.9896193771626, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=296, Percent_Identity=39.527027027027, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17554310, Length=328, Percent_Identity=25.9146341463415, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136226, Length=311, Percent_Identity=38.9067524115756, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=30.9904153354633, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24647881, Length=328, Percent_Identity=26.219512195122, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_CORGB (A4QHW5)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139733.1
- ProteinModelPortal:   A4QHW5
- SMR:   A4QHW5
- STRING:   A4QHW5
- GeneID:   4993669
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2812
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   PRK00066
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34362; Mature: 34362

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 179-179 BINDING 92-92 BINDING 124-124 BINDING 155-155 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETVGNKIVLIGAGDVGVAYAYALINQGMADHLAIIDIDEKKLEGNVMDLNHGVVWADS
CCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCEEEECCCEEEECC
RTRVTKGTYADCEDAAMVVICAGAAQKPGETRLQLVDKNVKIMKSIVGDVMASGFDGIFL
CCEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEE
VASNPVDILTYAVWKFSGLEWNRVIGSGTVLDSARFRYMLGELYEVAPSSVHAYIIGEHG
EECCCHHHHHHHHHHCCCCEEEHEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DTELPVLSSATIAGVSLSRMLDKDPELEGRLEKIFEDTRDAAYHIIDAKGSTSYGIGMGL
CCCCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHH
ARITRAILQNQDVAVPVSALLHGEYGEEDIYIGTPAVVNRRGIRRVVELEITDHEMERFK
HHHHHHHHCCCCCEEEHHHHHCCCCCCCCEEECCCHHHHHHHCEEEEEEEECHHHHHHHH
HSANTLREIQKQFF
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKETVGNKIVLIGAGDVGVAYAYALINQGMADHLAIIDIDEKKLEGNVMDLNHGVVWADS
CCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCEEEECCCEEEECC
RTRVTKGTYADCEDAAMVVICAGAAQKPGETRLQLVDKNVKIMKSIVGDVMASGFDGIFL
CCEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEE
VASNPVDILTYAVWKFSGLEWNRVIGSGTVLDSARFRYMLGELYEVAPSSVHAYIIGEHG
EECCCHHHHHHHHHHCCCCEEEHEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DTELPVLSSATIAGVSLSRMLDKDPELEGRLEKIFEDTRDAAYHIIDAKGSTSYGIGMGL
CCCCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHH
ARITRAILQNQDVAVPVSALLHGEYGEEDIYIGTPAVVNRRGIRRVVELEITDHEMERFK
HHHHHHHHCCCCCEEEHHHHHCCCCCCCCEEECCCHHHHHHHCEEEEEEEECHHHHHHHH
HSANTLREIQKQFF
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA