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The map label for this gene is dacB [H]

Identifier: 145296695

GI number: 145296695

Start: 2871495

End: 2872778

Strand: Reverse

Name: dacB [H]

Synonym: cgR_2600

Alternate gene names: 145296695

Gene position: 2872778-2871495 (Counterclockwise)

Preceding gene: 145296698

Following gene: 145296694

Centisome position: 86.68

GC content: 56.15

Gene sequence:

>1284_bases
ATGAAAAATGCGTGGTGGGTTGGCTCATCGGTTGGTGTGCTGATTGCAGTGGGGGCTGTCATCGGTGGTGGCGTGTGGGT
GAATCATTCTGGTTTTGGTTTGGATCACCCGGAGCCCATGTCGGTGGAGATGCCTGAGCAGCTGTTTTCTTCTGCGATTG
ATCCGGATGCGACGGAAGCCCCAGATTTTGCCACTTTGGAGAAGGATTTGACCTCGCAGGCTGCGGATTCTCGGTTGGGC
ACTTTTGTCGGTGTTGCCAGGGATGTGGAATCTGGTGAGGTGGTGTGGGAGCAGAATCAGGGGACTGCGGTGAGGCCGGC
GTCGGCGACGAAGATTCTGACGGCGGCGGTGGCTTTGTATGAGCTTGGCCGTGAGGACACCATTAAGACGGAGGTTGTTG
AGGGGGAGCAGCCGGGAACGGTGGTGATTAAGGCGGGTGGTGATGTCACGTTGAGTGAGGAGATGCTCGATGATTTGGCC
ACCCAGCTTGAGGGGCAAGATATTGACACTGTGTTGATCGATACGTCTATATGGCCTGATGAGGGCTTTGCTAGTACGTG
GGATCCAGTGGATGTTGATGCTGGTTATATCGCCGATGTGGAGCCCGCGATGATCGAGGCTGCCCGCATTGGTGGGTCGG
AGGGGGATCTGCCGAGGTCACATACCCCTGCGTTAGATGTTGCGCAGGCGTTGGCGGATCGTGTCGGCGCGGACACCGTA
GATGAGGGCAGCGCTCCGGACAAAACCGTGCTGGCATCCGTGGAGTCTGACACTTTGGATCAGCGTCTGGCTCGGATGAT
GAAGGATTCTGACAATGTGATGGCGGAGGGTATCGCTAAGGAGGTGGCTGCATCGAAGGATTTGGCGACCGATTCGGCGA
GTACTTCGAAGATGACGTTGGAAATTCTCAAGGACAAGGGCTTCGATTTGAGTGGCGTGTCCATTGTGGATAATTCGGGT
TTGAGCTTTGACAACCTCATTACGCCTCGTCTGCTTGATGATATTTTGACTCGCGCCGCCACGGAACCTGAGTTGAGTTC
ACTGCTGACATCGCTGCCGATCGCACATGGAACCGGAACCTTGGAGGATCGCTACGTCGGACTCTCGGGTGCGGGTTGGG
TGCGGGCGAAAACTGGCACTCTGACGGATACATCGGCATTGGCAGGGGTGGTGACCTCGGAGTCGGGGCGTGTGTTTACC
TTTGCTTTTGTGTCTAATGGTTCGGCGATTGTGCCGGCGCGTGAGGCTTTGGATGAGTTGGCGTCGATTCTGAGGGACTT
TTAA

Upstream 100 bases:

>100_bases
CCGCGCGTATGGTTCGTGCGTGAGACGACGGTCGATATAAGGTTGGAGTCCTGATATCGCAAGAAGAATCGCAAGAGAAT
TTGCAGGAGAAGGAGCGCCC

Downstream 100 bases:

>100_bases
GGTGGCATCCCTCATCGGGAATCTTGAGCTGCCTAGGGTAAGCCCTAATTTCTTGGAATTACGCAAGGCGGTGCGCCCTT
ACCTGAAAGAGCATGTGCAC

Product: hypothetical protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKNAWWVGSSVGVLIAVGAVIGGGVWVNHSGFGLDHPEPMSVEMPEQLFSSAIDPDATEAPDFATLEKDLTSQAADSRLG
TFVGVARDVESGEVVWEQNQGTAVRPASATKILTAAVALYELGREDTIKTEVVEGEQPGTVVIKAGGDVTLSEEMLDDLA
TQLEGQDIDTVLIDTSIWPDEGFASTWDPVDVDAGYIADVEPAMIEAARIGGSEGDLPRSHTPALDVAQALADRVGADTV
DEGSAPDKTVLASVESDTLDQRLARMMKDSDNVMAEGIAKEVAASKDLATDSASTSKMTLEILKDKGFDLSGVSIVDNSG
LSFDNLITPRLLDDILTRAATEPELSSLLTSLPIAHGTGTLEDRYVGLSGAGWVRAKTGTLTDTSALAGVVTSESGRVFT
FAFVSNGSAIVPAREALDELASILRDF

Sequences:

>Translated_427_residues
MKNAWWVGSSVGVLIAVGAVIGGGVWVNHSGFGLDHPEPMSVEMPEQLFSSAIDPDATEAPDFATLEKDLTSQAADSRLG
TFVGVARDVESGEVVWEQNQGTAVRPASATKILTAAVALYELGREDTIKTEVVEGEQPGTVVIKAGGDVTLSEEMLDDLA
TQLEGQDIDTVLIDTSIWPDEGFASTWDPVDVDAGYIADVEPAMIEAARIGGSEGDLPRSHTPALDVAQALADRVGADTV
DEGSAPDKTVLASVESDTLDQRLARMMKDSDNVMAEGIAKEVAASKDLATDSASTSKMTLEILKDKGFDLSGVSIVDNSG
LSFDNLITPRLLDDILTRAATEPELSSLLTSLPIAHGTGTLEDRYVGLSGAGWVRAKTGTLTDTSALAGVVTSESGRVFT
FAFVSNGSAIVPAREALDELASILRDF
>Mature_427_residues
MKNAWWVGSSVGVLIAVGAVIGGGVWVNHSGFGLDHPEPMSVEMPEQLFSSAIDPDATEAPDFATLEKDLTSQAADSRLG
TFVGVARDVESGEVVWEQNQGTAVRPASATKILTAAVALYELGREDTIKTEVVEGEQPGTVVIKAGGDVTLSEEMLDDLA
TQLEGQDIDTVLIDTSIWPDEGFASTWDPVDVDAGYIADVEPAMIEAARIGGSEGDLPRSHTPALDVAQALADRVGADTV
DEGSAPDKTVLASVESDTLDQRLARMMKDSDNVMAEGIAKEVAASKDLATDSASTSKMTLEILKDKGFDLSGVSIVDNSG
LSFDNLITPRLLDDILTRAATEPELSSLLTSLPIAHGTGTLEDRYVGLSGAGWVRAKTGTLTDTSALAGVVTSESGRVFT
FAFVSNGSAIVPAREALDELASILRDF

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=170, Percent_Identity=33.5294117647059, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44785; Mature: 44785

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAWWVGSSVGVLIAVGAVIGGGVWVNHSGFGLDHPEPMSVEMPEQLFSSAIDPDATEA
CCCCEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
PDFATLEKDLTSQAADSRLGTFVGVARDVESGEVVWEQNQGTAVRPASATKILTAAVALY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHH
ELGREDTIKTEVVEGEQPGTVVIKAGGDVTLSEEMLDDLATQLEGQDIDTVLIDTSIWPD
HCCCCCCCEEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCC
EGFASTWDPVDVDAGYIADVEPAMIEAARIGGSEGDLPRSHTPALDVAQALADRVGADTV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DEGSAPDKTVLASVESDTLDQRLARMMKDSDNVMAEGIAKEVAASKDLATDSASTSKMTL
CCCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
EILKDKGFDLSGVSIVDNSGLSFDNLITPRLLDDILTRAATEPELSSLLTSLPIAHGTGT
EEHHCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
LEDRYVGLSGAGWVRAKTGTLTDTSALAGVVTSESGRVFTFAFVSNGSAIVPAREALDEL
CCCEEEECCCCCEEEECCCCCCCHHHHHEEEECCCCCEEEEEEECCCCEECCHHHHHHHH
ASILRDF
HHHHHCC
>Mature Secondary Structure
MKNAWWVGSSVGVLIAVGAVIGGGVWVNHSGFGLDHPEPMSVEMPEQLFSSAIDPDATEA
CCCCEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
PDFATLEKDLTSQAADSRLGTFVGVARDVESGEVVWEQNQGTAVRPASATKILTAAVALY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCHHHHHHHHHHHHH
ELGREDTIKTEVVEGEQPGTVVIKAGGDVTLSEEMLDDLATQLEGQDIDTVLIDTSIWPD
HCCCCCCCEEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCCC
EGFASTWDPVDVDAGYIADVEPAMIEAARIGGSEGDLPRSHTPALDVAQALADRVGADTV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DEGSAPDKTVLASVESDTLDQRLARMMKDSDNVMAEGIAKEVAASKDLATDSASTSKMTL
CCCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
EILKDKGFDLSGVSIVDNSGLSFDNLITPRLLDDILTRAATEPELSSLLTSLPIAHGTGT
EEHHCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
LEDRYVGLSGAGWVRAKTGTLTDTSALAGVVTSESGRVFTFAFVSNGSAIVPAREALDEL
CCCEEEECCCCCEEEECCCCCCCHHHHHEEEECCCCCEEEEEEECCCCEECCHHHHHHHH
ASILRDF
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]