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The map label for this gene is speE [H]

Identifier: 145296698

GI number: 145296698

Start: 2873956

End: 2875497

Strand: Reverse

Name: speE [H]

Synonym: cgR_2603

Alternate gene names: 145296698

Gene position: 2875497-2873956 (Counterclockwise)

Preceding gene: 145296699

Following gene: 145296695

Centisome position: 86.76

GC content: 55.9

Gene sequence:

>1542_bases
ATGTCTGATTTAGGACCCATCTGGCGCTGGCTGTTATTAGTTTCCGTCTCCATTTGTGCGGCATCGGGGCTGGTCTATGA
GCTAGCCCTGGTATCCCTTTCCACCAGCTTGAACGGTGGCGGAATTGTAGAAACCTCCCTCATCGTCGCAGGTTATGTAG
CTGCCCTTGGACTTGGTGCACTGCTGGTCAAGCCGTTTCTCAACTGGCCTGCACAAACCTTCCTCGGTGTGGAAACCTTG
CTGGGACTCATCGGTGGTTGCTCCGCGCTGGTGCTATATTTCACCTTTGCGACCATCGGCCAGTCCTTGTGGATTCTGGT
GATTGCCACCGCTGCAATTGGCATCCTGGTCGGCGCTGAACTTCCGCTGCTGATGACCATGATCCAGCAAGGCCGCCTCG
CCGACGCCAAAACCACAGGATCTCTGGTTGCCACCTTGAATGCTGCTGATTACCTTGGCGCACTTTTAGGTGGCCTGGCC
TGGCCTTTTGTGTTGCTGCCGTGGCTTGGCATGATGCGCGGTGCCGCAGCAGCCGGAATGATCAACCTCGTTGCAGCACT
ATTCGTGGGCTGTGTGCTGCTGCGACATTTGCTTCCGCGCACCCACTTCTTCGTATCCGTGGTGGCGCTTCTTCTCGCGA
TCACAGCGCTTGCCACCGTGTTGGTGAAATCCGACGGGATCGTTGCCACCGCCCGCGCGCAGCTCTACCGCGACCCCGTG
ATCTATTCACACCAATCTGACTACCAAGACATCGTAGTGACAGAACGAGGCAAAGACCGACGCCTCTACCTCAATGGCGG
TTTGCAGTATTCCACTCGTGACCAGCATAGATATACAGAATCACTGGTGTATCCAAGCCTTAATCCAGAGGCAGAATCGG
TGTTAATCATAGGCGGTGGCGATGGCCTCGTAGCACGCGAACTTCTCCGCTTTCCTTCCATGCAGATCACACAAGTGGAA
CTAGACCCAGAAGTCATCGAAGTAGCCAACACTGTGCTGCGCTCTGATAACGGGGGAGCGATGGAAGACCCCCGCGTGTC
CATCATCGTCGACGACGCCTTCACCTGGCTACGCTCCGGCGGAAACAACGGCGAAACTTACGATTCCATCATCATCGATC
TTCCCGATCCCAACAACGACACCATGGCCAGGCTGTATTCCGAAGAGTTCTACACCTTGGCTCGAGCACGACTGAAGGAA
CACGGACGCATGGTGGTGCAATCCTCCAGCGCGTACACCACCCCAGATGTGTTTTGGCGTGTTGGAGCAACCTTGGAATC
GGCGGGCTGCGAACAAGTCATCCCGTATCACGTGCATGTTCCCACATTTGGTGACTGGGGCTTCCAATTGTGCGGACCTA
CCGACATGGAATTAGAGCTTCGGGAAGACACTCCGCCGCTGACTTTCCTCAATGATGAGGTTTTGGTGGCTGCTGCGGTG
TTCGGGTTGGATAATCAGCCTCGCAAATTAGAGCCCTCCACGCTGGATCACCCACGCGTGGTGGAGGATCTGCGCAAGGG
ATACCGCGAATCAGGTGACTAG

Upstream 100 bases:

>100_bases
TTCCGGGACGTTTCCGTGATCTCGTGGAAGATCCCAAACTCCGTTCCGGTGCCGTCGTGGCCGCCGTCATCTTGATCGTG
GTGGGAACCGTAAACGCTGC

Downstream 100 bases:

>100_bases
CTGCAACGATGTGCTGTGTGGCGATCTAAGCGGTCGATATTTTCGAATGAGTATTTGTATCGGGATGGGTGTGAAAGTCC
CTTAAATCGCATTCTGTGAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGALLVKPFLNWPAQTFLGVETL
LGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAELPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLA
WPFVLLPWLGMMRGAAAAGMINLVAALFVGCVLLRHLLPRTHFFVSVVALLLAITALATVLVKSDGIVATARAQLYRDPV
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGGDGLVARELLRFPSMQITQVE
LDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSGGNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLKE
HGRMVVQSSSAYTTPDVFWRVGATLESAGCEQVIPYHVHVPTFGDWGFQLCGPTDMELELREDTPPLTFLNDEVLVAAAV
FGLDNQPRKLEPSTLDHPRVVEDLRKGYRESGD

Sequences:

>Translated_513_residues
MSDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGALLVKPFLNWPAQTFLGVETL
LGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAELPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLA
WPFVLLPWLGMMRGAAAAGMINLVAALFVGCVLLRHLLPRTHFFVSVVALLLAITALATVLVKSDGIVATARAQLYRDPV
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGGDGLVARELLRFPSMQITQVE
LDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSGGNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLKE
HGRMVVQSSSAYTTPDVFWRVGATLESAGCEQVIPYHVHVPTFGDWGFQLCGPTDMELELREDTPPLTFLNDEVLVAAAV
FGLDNQPRKLEPSTLDHPRVVEDLRKGYRESGD
>Mature_512_residues
SDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGALLVKPFLNWPAQTFLGVETLL
GLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAELPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLAW
PFVLLPWLGMMRGAAAAGMINLVAALFVGCVLLRHLLPRTHFFVSVVALLLAITALATVLVKSDGIVATARAQLYRDPVI
YSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGGDGLVARELLRFPSMQITQVEL
DPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSGGNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLKEH
GRMVVQSSSAYTTPDVFWRVGATLESAGCEQVIPYHVHVPTFGDWGFQLCGPTDMELELREDTPPLTFLNDEVLVAAAVF
GLDNQPRKLEPSTLDHPRVVEDLRKGYRESGD

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=239, Percent_Identity=29.7071129707113, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786312, Length=179, Percent_Identity=31.8435754189944, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25154106, Length=177, Percent_Identity=33.8983050847458, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI212645616, Length=172, Percent_Identity=34.3023255813954, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323175, Length=184, Percent_Identity=32.6086956521739, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325326, Length=246, Percent_Identity=28.4552845528455, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24645443, Length=197, Percent_Identity=30.9644670050761, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355941, Length=146, Percent_Identity=29.4520547945205, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 55596; Mature: 55465

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLVKPFLNWPAQTFLGVETLLGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLAWPFVLLPWLGMMRGAAAAGM
HHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INLVAALFVGCVLLRHLLPRTHFFVSVVALLLAITALATVLVKSDGIVATARAQLYRDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCE
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGG
EECCCCCCCEEEEEECCCCCEEEEECCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECC
DGLVARELLRFPSMQITQVELDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSG
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHCC
GNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLKEHGRMVVQSSSAYTTPDVFWR
CCCCCEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
VGATLESAGCEQVIPYHVHVPTFGDWGFQLCGPTDMELELREDTPPLTFLNDEVLVAAAV
HCCHHHHCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEECCCCCCEEEECCHHHHHHHH
FGLDNQPRKLEPSTLDHPRVVEDLRKGYRESGD
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDLGPIWRWLLLVSVSICAASGLVYELALVSLSTSLNGGGIVETSLIVAGYVAALGLGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLVKPFLNWPAQTFLGVETLLGLIGGCSALVLYFTFATIGQSLWILVIATAAIGILVGAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPLLMTMIQQGRLADAKTTGSLVATLNAADYLGALLGGLAWPFVLLPWLGMMRGAAAAGM
HHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INLVAALFVGCVLLRHLLPRTHFFVSVVALLLAITALATVLVKSDGIVATARAQLYRDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCE
IYSHQSDYQDIVVTERGKDRRLYLNGGLQYSTRDQHRYTESLVYPSLNPEAESVLIIGGG
EECCCCCCCEEEEEECCCCCEEEEECCEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECC
DGLVARELLRFPSMQITQVELDPEVIEVANTVLRSDNGGAMEDPRVSIIVDDAFTWLRSG
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHCC
GNNGETYDSIIIDLPDPNNDTMARLYSEEFYTLARARLKEHGRMVVQSSSAYTTPDVFWR
CCCCCEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
VGATLESAGCEQVIPYHVHVPTFGDWGFQLCGPTDMELELREDTPPLTFLNDEVLVAAAV
HCCHHHHCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEECCCCCCEEEECCHHHHHHHH
FGLDNQPRKLEPSTLDHPRVVEDLRKGYRESGD
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]