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The map label for this gene is tilS [H]

Identifier: 145296694

GI number: 145296694

Start: 2870549

End: 2871442

Strand: Reverse

Name: tilS [H]

Synonym: cgR_2599

Alternate gene names: 145296694

Gene position: 2871442-2870549 (Counterclockwise)

Preceding gene: 145296695

Following gene: 145296693

Centisome position: 86.64

GC content: 60.74

Gene sequence:

>894_bases
TTGGAATTACGCAAGGCGGTGCGCCCTTACCTGAAAGAGCATGTGCACATTGGGTTGTCGGGCGGGCCGGATTCTTTGGC
GCTGGTGGCTGCTGTGCTGGCGGAGAAATCCCAGGTCACGGCGATTTGTATTGATCACAATCTGCAGGCCGGTTCTGCTG
AAGTCACGCACAACGCTGCTGCGATGGCGCGCCACATGGGCGCACAGGCGATCGTGAAGAGCATCGAGGTCGCGCCGGGG
GAGGGGATGGAGGCCGCCGCCAGGGAGGCTCGGTACGCGGCTTTTGCGCAGCTCACCGATGAGATTTGGGTGGCGCACAC
CATGGATGATCAAGCCGAGACCTATCTCCTTGGCGGTTTGCGGGGGAATCCCGCGGGCATGAAAGATGCTTCTCGACGCC
CTGAGCTATCCATTATTCGACCCCTTCTGGGGGCTCGGCGTGCGCACACGCACGGGGCGTGCGTGGAGTTGGGGTTGAAA
CCGTGGCACGATCCGCAAAATTTTGACGATGCCTTTCGGCGGGTAGCCATCCGAAACCAGGTGATTCCTCTTCTTGCGCA
GGTGCACGGGGGAGACCCTGTACCTGGTTTGGCACTTGCGGCGCGACGCGCTGTGGAGGATGCCGAAGTGGTGGAGGGCG
ACGTCGAGAAGCGGCGTTTGGAGTGGCAGGACGGTTTTCCTGTGGCGTTGGCCGGGGAACCTACGGGCCTTAGGCGACGC
ATGTTGGCGGATTTTTTGCGTGGGGAAGGCATCGCTGTGAGCTCGAGGAAACTCGCCGCCATTGACCGATTGCTCACCGA
TTGGCGCGGACAGGGTGGCGTAGCCGTGGGTAAAAGCGATAATGGAAGGTTGGAAGTGGTGCGGCAAAGTGGCAAGCTTA
AGATCACTGATTGA

Upstream 100 bases:

>100_bases
GCGCGTGAGGCTTTGGATGAGTTGGCGTCGATTCTGAGGGACTTTTAAGGTGGCATCCCTCATCGGGAATCTTGAGCTGC
CTAGGGTAAGCCCTAATTTC

Downstream 100 bases:

>100_bases
CACCTGAATCTACAACACAAGGGGAACGCGACGATGAGTAACAACGTAGAAATGGCCGACCACAAAGATCTCAATGTTCC
AGCCAACCCATACGGCACCG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQAGSAEVTHNAAAMARHMGAQAIVKSIEVAPG
EGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLLGARRAHTHGACVELGLK
PWHDPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQDGFPVALAGEPTGLRRR
MLADFLRGEGIAVSSRKLAAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD

Sequences:

>Translated_297_residues
MELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQAGSAEVTHNAAAMARHMGAQAIVKSIEVAPG
EGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLLGARRAHTHGACVELGLK
PWHDPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQDGFPVALAGEPTGLRRR
MLADFLRGEGIAVSSRKLAAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD
>Mature_297_residues
MELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQAGSAEVTHNAAAMARHMGAQAIVKSIEVAPG
EGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLLGARRAHTHGACVELGLK
PWHDPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQDGFPVALAGEPTGLRRR
MLADFLRGEGIAVSSRKLAAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=188, Percent_Identity=34.0425531914894, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 32017; Mature: 32017

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQAGSAEVTHNAA
CCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
AMARHMGAQAIVKSIEVAPGEGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECC
RGNPAGMKDASRRPELSIIRPLLGARRAHTHGACVELGLKPWHDPQNFDDAFRRVAIRNQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
VIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQDGFPVALAGEPTGLRRR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEECCCCHHHHHH
MLADFLRGEGIAVSSRKLAAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD
HHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCEEECC
>Mature Secondary Structure
MELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQAGSAEVTHNAA
CCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
AMARHMGAQAIVKSIEVAPGEGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECC
RGNPAGMKDASRRPELSIIRPLLGARRAHTHGACVELGLKPWHDPQNFDDAFRRVAIRNQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
VIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQDGFPVALAGEPTGLRRR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEECCCCHHHHHH
MLADFLRGEGIAVSSRKLAAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD
HHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]