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The map label for this gene is rsmE [H]

Identifier: 145296244

GI number: 145296244

Start: 2383623

End: 2384348

Strand: Reverse

Name: rsmE [H]

Synonym: cgR_2161

Alternate gene names: 145296244

Gene position: 2384348-2383623 (Counterclockwise)

Preceding gene: 145296245

Following gene: 145296243

Centisome position: 71.94

GC content: 60.33

Gene sequence:

>726_bases
ATGTCACTGCCAGTATTTATCTCTGATTCCGCAGCCGGACCAGGCGAAACCATTGAGCTCGCCGGTCCGGAAGGCCGGCA
CGCCGTCACCGTCAAACGGATCCAGGTAGGCGAAAAGATTTCGCTTATCGACGGCCGGGGTACAGCGCGCACCTGCACGG
TCACCGCCTTGCAGGGCAAAGACCACCTGAACGCTGTGGTGGATTTGGTCGAGGAAATCCCCGCACCGAACCCACAGGTC
ACCATCGTGCAGGCTATCCCCAAATCAGAGCGTTCCGAGCTGACCATCGACCTGCTCACCCAAGCTGGCGCCGATCGGAT
TGTTGCGTGGCAAGCATCGCGCTGCGTAGCAAAATGGGGCGGCAAGGAAGCAAAATCACTGGCTAAATGGCAGGTGGCTG
CGGAAGCAGCCGCCAAGCAATCGCGGCGCGCAACGATCCCTGAAATTCTTGGCGTAGTGGGGGAGGAGGGCGTCGAAAAG
CTTATCGACGAATCCGACCTGGCCATCATCCTGCACGAAGAAGCAACCGCCGCGATCCGTGAACTGGAATTTTCCGGCAA
CGTCGTGGTGATCATCGGACCCGAAGGTGGCGTCGCGCCATCGGAAATTTCACGCTTCGTCGACGCTGGCGCACACACCG
TGAAACTGGGCCCTGAGGTGTTGCGCACTGCCTCCGCAGGCATGGTGGCGCTCGCAGCTATCGGCGTGCTGTCTGACCGC
TGGTAA

Upstream 100 bases:

>100_bases
AAGAAATCCGCAACCATCGCAGCGACAACGCTTCCGTGCATCGCGAAGGCGGAGAAGAATCCGGTTTCTTTGACAAGCTC
CGAAACAAGTTCCGCAAATA

Downstream 100 bases:

>100_bases
CATTTCTCTGACACAACCTTTCTTGAACATTCCGAAAGCAGGCCGAACTAGTACTGTGACTGCCCCGCGAAAAACCCGCA
CAGAAGTAATCACCACTGTC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSLPVFISDSAAGPGETIELAGPEGRHAVTVKRIQVGEKISLIDGRGTARTCTVTALQGKDHLNAVVDLVEEIPAPNPQV
TIVQAIPKSERSELTIDLLTQAGADRIVAWQASRCVAKWGGKEAKSLAKWQVAAEAAAKQSRRATIPEILGVVGEEGVEK
LIDESDLAIILHEEATAAIRELEFSGNVVVIIGPEGGVAPSEISRFVDAGAHTVKLGPEVLRTASAGMVALAAIGVLSDR
W

Sequences:

>Translated_241_residues
MSLPVFISDSAAGPGETIELAGPEGRHAVTVKRIQVGEKISLIDGRGTARTCTVTALQGKDHLNAVVDLVEEIPAPNPQV
TIVQAIPKSERSELTIDLLTQAGADRIVAWQASRCVAKWGGKEAKSLAKWQVAAEAAAKQSRRATIPEILGVVGEEGVEK
LIDESDLAIILHEEATAAIRELEFSGNVVVIIGPEGGVAPSEISRFVDAGAHTVKLGPEVLRTASAGMVALAAIGVLSDR
W
>Mature_240_residues
SLPVFISDSAAGPGETIELAGPEGRHAVTVKRIQVGEKISLIDGRGTARTCTVTALQGKDHLNAVVDLVEEIPAPNPQVT
IVQAIPKSERSELTIDLLTQAGADRIVAWQASRCVAKWGGKEAKSLAKWQVAAEAAAKQSRRATIPEILGVVGEEGVEKL
IDESDLAIILHEEATAAIRELEFSGNVVVIIGPEGGVAPSEISRFVDAGAHTVKLGPEVLRTASAGMVALAAIGVLSDRW

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=239, Percent_Identity=26.7782426778243, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25273; Mature: 25142

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPVFISDSAAGPGETIELAGPEGRHAVTVKRIQVGEKISLIDGRGTARTCTVTALQGK
CCCCEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCEEEEEECCCCCEEEEEEEECCC
DHLNAVVDLVEEIPAPNPQVTIVQAIPKSERSELTIDLLTQAGADRIVAWQASRCVAKWG
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEHHHCCCCEEEEEHHHHHHHHCC
GKEAKSLAKWQVAAEAAAKQSRRATIPEILGVVGEEGVEKLIDESDLAIILHEEATAAIR
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHCCCCEEEEEECCHHHHHH
ELEFSGNVVVIIGPEGGVAPSEISRFVDAGAHTVKLGPEVLRTASAGMVALAAIGVLSDR
HEECCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHCCCHHHHHHHHHHCCC
W
C
>Mature Secondary Structure 
SLPVFISDSAAGPGETIELAGPEGRHAVTVKRIQVGEKISLIDGRGTARTCTVTALQGK
CCCEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCEEEEEECCCCCEEEEEEEECCC
DHLNAVVDLVEEIPAPNPQVTIVQAIPKSERSELTIDLLTQAGADRIVAWQASRCVAKWG
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEHHHCCCCEEEEEHHHHHHHHCC
GKEAKSLAKWQVAAEAAAKQSRRATIPEILGVVGEEGVEKLIDESDLAIILHEEATAAIR
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHCCCCEEEEEECCHHHHHH
ELEFSGNVVVIIGPEGGVAPSEISRFVDAGAHTVKLGPEVLRTASAGMVALAAIGVLSDR
HEECCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHCCCHHHHHHHHHHCCC
W
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]