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The map label for this gene is hemK [H]

Identifier: 145295340

GI number: 145295340

Start: 1411276

End: 1412115

Strand: Direct

Name: hemK [H]

Synonym: cgR_1280

Alternate gene names: 145295340

Gene position: 1411276-1412115 (Clockwise)

Preceding gene: 145295339

Following gene: 145295341

Centisome position: 42.58

GC content: 58.57

Gene sequence:

>840_bases
ATGCTGACCCTTGGGGAAGCTTTGCGTGACGCCACCGCCACCCTGGAAAGGGCGGGGGTGGCGTCGCCACTTGTGGACGC
TCGTTTGATTGCGGCTCATTTGCTGGGTTGCGGTCCGCTCGATATTGCATTGCGCATGCGTGATGAGGTGCCGGCTGGGT
TTGCGGCGGCGGTTGAGCGTCGTGCGCGACGCGAACCGCTGCAGCACATCTTGGGCACGGCACCCATGGGCCCGTTGGAT
TTGCATGTGGGACCGGGCGTGTTCATTCCGCGCCCGGAAACCGAGGTGCTCGCGGACTGGGCCGTGCGGCAGGTGGCTGG
CGACGTCGAAAAGCGAAAAATCGTTGACCTGTGCACCGGATCCGGCGCGCTTGCGGCCTACATCGGGCATGCGCTTATCG
ACGCGACCCTCTACGCTGTCGAACTCGATCCGGGGGCAGCGACCTGGGCGCAGCGAAACTTCGATGAATTTGCACCGCAG
GTGAAGCTCATCCACGGTGATGTAACAGATCCGACATTGTTGGCTGAGGTGCATGGCACGATCGATCTCGTTGTGTCAAA
TCCGCCGTATGTGCCTGAATCTGATGATTTGGATCCAGAGGTCTACCAAGATCCACACATGGCGGTATTTAGTGGCGCTG
ATGGCATGGATGTCATCAACAAGATGGTGCACCTGATCTTCAATTTGCTGAAATCTGGTGGGGCAGTGGGCATCGAACAT
GACGACACCACTTCCGACGCCGTCCGCCAAGTTTTTTCTCAGCACGGGGGATTTGGCACTATTGAAGTGCTTCATGATCT
CACCGGCAGAGCGCGTTTTGTTACAGCGCGTAAACTGTGA

Upstream 100 bases:

>100_bases
GGCGAACAACCTTGATTCGGTTCTCGATGGTGAATTGGATGATCTGTTCACCGCGTTGCAGGCTGCTGAGCGTGCAGAAC
GTCTTGAGGCCGAGGGCTAA

Downstream 100 bases:

>100_bases
CTTTGAAAAATTTTTGAACAATCCGTACACCAACTCTAGGAGAAAAACAGTGAGCAGAATCTATGACTGTGCCGACCAAG
ACTCCCGTGCAGCAGGCCTA

Product: hypothetical protein

Products: NA

Alternate protein names: M.MtuHHemKP [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLTLGEALRDATATLERAGVASPLVDARLIAAHLLGCGPLDIALRMRDEVPAGFAAAVERRARREPLQHILGTAPMGPLD
LHVGPGVFIPRPETEVLADWAVRQVAGDVEKRKIVDLCTGSGALAAYIGHALIDATLYAVELDPGAATWAQRNFDEFAPQ
VKLIHGDVTDPTLLAEVHGTIDLVVSNPPYVPESDDLDPEVYQDPHMAVFSGADGMDVINKMVHLIFNLLKSGGAVGIEH
DDTTSDAVRQVFSQHGGFGTIEVLHDLTGRARFVTARKL

Sequences:

>Translated_279_residues
MLTLGEALRDATATLERAGVASPLVDARLIAAHLLGCGPLDIALRMRDEVPAGFAAAVERRARREPLQHILGTAPMGPLD
LHVGPGVFIPRPETEVLADWAVRQVAGDVEKRKIVDLCTGSGALAAYIGHALIDATLYAVELDPGAATWAQRNFDEFAPQ
VKLIHGDVTDPTLLAEVHGTIDLVVSNPPYVPESDDLDPEVYQDPHMAVFSGADGMDVINKMVHLIFNLLKSGGAVGIEH
DDTTSDAVRQVFSQHGGFGTIEVLHDLTGRARFVTARKL
>Mature_279_residues
MLTLGEALRDATATLERAGVASPLVDARLIAAHLLGCGPLDIALRMRDEVPAGFAAAVERRARREPLQHILGTAPMGPLD
LHVGPGVFIPRPETEVLADWAVRQVAGDVEKRKIVDLCTGSGALAAYIGHALIDATLYAVELDPGAATWAQRNFDEFAPQ
VKLIHGDVTDPTLLAEVHGTIDLVVSNPPYVPESDDLDPEVYQDPHMAVFSGADGMDVINKMVHLIFNLLKSGGAVGIEH
DDTTSDAVRQVFSQHGGFGTIEVLHDLTGRARFVTARKL

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=294, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787463, Length=279, Percent_Identity=31.5412186379928, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082085, Length=214, Percent_Identity=28.9719626168224, Blast_Score=68, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324266, Length=195, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582226, Length=285, Percent_Identity=29.8245614035088, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 29881; Mature: 29881

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLGEALRDATATLERAGVASPLVDARLIAAHLLGCGPLDIALRMRDEVPAGFAAAVER
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHHHHH
RARREPLQHILGTAPMGPLDLHVGPGVFIPRPETEVLADWAVRQVAGDVEKRKIVDLCTG
HHHHHHHHHHHCCCCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGALAAYIGHALIDATLYAVELDPGAATWAQRNFDEFAPQVKLIHGDVTDPTLLAEVHGT
CCHHHHHHHHHHHHHEEEEEEECCCCHHHHHCCHHHHCCEEEEEECCCCCCHHHHHHCCE
IDLVVSNPPYVPESDDLDPEVYQDPHMAVFSGADGMDVINKMVHLIFNLLKSGGAVGIEH
EEEEECCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCC
DDTTSDAVRQVFSQHGGFGTIEVLHDLTGRARFVTARKL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MLTLGEALRDATATLERAGVASPLVDARLIAAHLLGCGPLDIALRMRDEVPAGFAAAVER
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHHHHH
RARREPLQHILGTAPMGPLDLHVGPGVFIPRPETEVLADWAVRQVAGDVEKRKIVDLCTG
HHHHHHHHHHHCCCCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGALAAYIGHALIDATLYAVELDPGAATWAQRNFDEFAPQVKLIHGDVTDPTLLAEVHGT
CCHHHHHHHHHHHHHEEEEEEECCCCHHHHHCCHHHHCCEEEEEECCCCCCHHHHHHCCE
IDLVVSNPPYVPESDDLDPEVYQDPHMAVFSGADGMDVINKMVHLIFNLLKSGGAVGIEH
EEEEECCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCC
DDTTSDAVRQVFSQHGGFGTIEVLHDLTGRARFVTARKL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]