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The map label for this gene is prfA [H]

Identifier: 145295339

GI number: 145295339

Start: 1410199

End: 1411275

Strand: Direct

Name: prfA [H]

Synonym: cgR_1279

Alternate gene names: 145295339

Gene position: 1410199-1411275 (Clockwise)

Preceding gene: 145295338

Following gene: 145295340

Centisome position: 42.55

GC content: 55.15

Gene sequence:

>1077_bases
ATGGCATCGCAGGTTTCTGCAGTTGACGATATTTTGTCGGAGTACCACGGTCTGGAACAGCAGATGGCTGATCCGGAGTT
GCACAATGATGCGGCAGCAGCGCGCCGGGTAGGTAAGCGCTACTCGGAGTTGCAGCCGATCATTAACGTGCACCGTGATT
TGGTGTCTGCCCAGGAAGATCTTGAGGCAGCCCGTGAAATGGCACATGAGGACCATGAGTTCCAGGCCGAGGCTGAGCGC
CTTGAGGTGGAAGTTGTGGAGTTGGAAGAAAAGCTCGCTGATCTTCTTGCACCTCGTGATCCTCATGATGGCGAAGACAT
CGTGATGGAAATCAAGGCTGGTGCCGGTGGCGAAGAAGCAGCACTGTTTGCTGGCGATTTGCTGCGCATGTACCAGAAGT
TTGCAGACAAGCACGGCTTTGTCGTGGAAGTTTTGGACTCTGCAGAATCTGACCTCGGTGGTGTCAAGGACATCACCTTG
TCTATTCGTTCCCGTCAGCCATCCCGCGATGGTGCGTGGAGCCAGTTCAAGTTTGAAGGTGGCGTGCACCGTGTTCAGCG
TGTGCCTGTTACTGAATCTCAGGGACGCATCCAGACCTCTGCTGCTGGTGTCTTGGTTTACCCAGAACCAGATGAGGTGG
AAAACGTTGAGATCGATGAGAAGGATATTCGCGTCGATGTGTACCGTTCCTCAGGTAAGGGTGGTCAGGGCGTTAACACC
ACTGACTCCGCTGTGCGTATTACCCACTTGCCAACTGGTTTGGTGGTGACCTGTCAGAAGGAGCGCTCCCAGATTCAGAA
CCGTGCGCGTGCAATGCAGGTTTTGGCTGCCCGTTTGCAGGCGATGAAGGAAGAGGAAGCGGCGGCTGAGGCTGCTACGG
GACGTGCTGCGCAGATCCGCACCATGGACCGCTCTGAGCGCATCCGTACCTACAACTGGCCGGAAAACCGCATCAGCGAT
CACCGTATTGGTTTTAAGGCGAACAACCTTGATTCGGTTCTCGATGGTGAATTGGATGATCTGTTCACCGCGTTGCAGGC
TGCTGAGCGTGCAGAACGTCTTGAGGCCGAGGGCTAA

Upstream 100 bases:

>100_bases
TCAAGCAGCTGAAGAAGACCAAGTCCAACGCGGAATTCCTCATGCAGGTTGCTTCCAGCGCTCCAATGGCAGGCACAGAA
AAAGAGGAGGATTACTCCTA

Downstream 100 bases:

>100_bases
ATGCTGACCCTTGGGGAAGCTTTGCGTGACGCCACCGCCACCCTGGAAAGGGCGGGGGTGGCGTCGCCACTTGTGGACGC
TCGTTTGATTGCGGCTCATT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MASQVSAVDDILSEYHGLEQQMADPELHNDAAAARRVGKRYSELQPIINVHRDLVSAQEDLEAAREMAHEDHEFQAEAER
LEVEVVELEEKLADLLAPRDPHDGEDIVMEIKAGAGGEEAALFAGDLLRMYQKFADKHGFVVEVLDSAESDLGGVKDITL
SIRSRQPSRDGAWSQFKFEGGVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVENVEIDEKDIRVDVYRSSGKGGQGVNT
TDSAVRITHLPTGLVVTCQKERSQIQNRARAMQVLAARLQAMKEEEAAAEAATGRAAQIRTMDRSERIRTYNWPENRISD
HRIGFKANNLDSVLDGELDDLFTALQAAERAERLEAEG

Sequences:

>Translated_358_residues
MASQVSAVDDILSEYHGLEQQMADPELHNDAAAARRVGKRYSELQPIINVHRDLVSAQEDLEAAREMAHEDHEFQAEAER
LEVEVVELEEKLADLLAPRDPHDGEDIVMEIKAGAGGEEAALFAGDLLRMYQKFADKHGFVVEVLDSAESDLGGVKDITL
SIRSRQPSRDGAWSQFKFEGGVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVENVEIDEKDIRVDVYRSSGKGGQGVNT
TDSAVRITHLPTGLVVTCQKERSQIQNRARAMQVLAARLQAMKEEEAAAEAATGRAAQIRTMDRSERIRTYNWPENRISD
HRIGFKANNLDSVLDGELDDLFTALQAAERAERLEAEG
>Mature_357_residues
ASQVSAVDDILSEYHGLEQQMADPELHNDAAAARRVGKRYSELQPIINVHRDLVSAQEDLEAAREMAHEDHEFQAEAERL
EVEVVELEEKLADLLAPRDPHDGEDIVMEIKAGAGGEEAALFAGDLLRMYQKFADKHGFVVEVLDSAESDLGGVKDITLS
IRSRQPSRDGAWSQFKFEGGVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVENVEIDEKDIRVDVYRSSGKGGQGVNTT
DSAVRITHLPTGLVVTCQKERSQIQNRARAMQVLAARLQAMKEEEAAAEAATGRAAQIRTMDRSERIRTYNWPENRISDH
RIGFKANNLDSVLDGELDDLFTALQAAERAERLEAEG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=265, Percent_Identity=45.2830188679245, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI34577120, Length=354, Percent_Identity=34.4632768361582, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI166795305, Length=169, Percent_Identity=46.7455621301775, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1787462, Length=342, Percent_Identity=43.5672514619883, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI2367172, Length=331, Percent_Identity=37.4622356495468, Blast_Score=197, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=310, Percent_Identity=35.4838709677419, Blast_Score=157, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321295, Length=328, Percent_Identity=38.719512195122, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI19921226, Length=335, Percent_Identity=39.4029850746269, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39677; Mature: 39546

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQVSAVDDILSEYHGLEQQMADPELHNDAAAARRVGKRYSELQPIINVHRDLVSAQED
CCCHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAAREMAHEDHEFQAEAERLEVEVVELEEKLADLLAPRDPHDGEDIVMEIKAGAGGEEA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAGDLLRMYQKFADKHGFVVEVLDSAESDLGGVKDITLSIRSRQPSRDGAWSQFKFEG
HHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEECC
GVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVENVEIDEKDIRVDVYRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
TDSAVRITHLPTGLVVTCQKERSQIQNRARAMQVLAARLQAMKEEEAAAEAATGRAAQIR
CCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEE
TMDRSERIRTYNWPENRISDHRIGFKANNLDSVLDGELDDLFTALQAAERAERLEAEG
ECCHHCCCCCCCCCHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASQVSAVDDILSEYHGLEQQMADPELHNDAAAARRVGKRYSELQPIINVHRDLVSAQED
CCHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAAREMAHEDHEFQAEAERLEVEVVELEEKLADLLAPRDPHDGEDIVMEIKAGAGGEEA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAGDLLRMYQKFADKHGFVVEVLDSAESDLGGVKDITLSIRSRQPSRDGAWSQFKFEG
HHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEECC
GVHRVQRVPVTESQGRIQTSAAGVLVYPEPDEVENVEIDEKDIRVDVYRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
TDSAVRITHLPTGLVVTCQKERSQIQNRARAMQVLAARLQAMKEEEAAAEAATGRAAQIR
CCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEE
TMDRSERIRTYNWPENRISDHRIGFKANNLDSVLDGELDDLFTALQAAERAERLEAEG
ECCHHCCCCCCCCCHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]