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The map label for this gene is citE [H]

Identifier: 145295033

GI number: 145295033

Start: 1086156

End: 1086977

Strand: Direct

Name: citE [H]

Synonym: cgR_0977

Alternate gene names: 145295033

Gene position: 1086156-1086977 (Clockwise)

Preceding gene: 145295032

Following gene: 145295042

Centisome position: 32.77

GC content: 52.68

Gene sequence:

>822_bases
ATGTCTGAACTTATTTGTGGACCAGCTATTCTCTTCGCACCAGCTGGACGTGCTGAGATCATTCCAAAAGCAGCATCGAA
GGCCGATATGGTCATCATTGATTTGGAAGATGGGGCAGGGGAGGTAGACCGTGAGGTCGCCTACAGGAACATTAGGGAAT
CGGGGTTGGATCCTAAACGAACCATTGTGAGAACCGTAGGGCCGAGCGATCCACACTTTTTGGCTGACGTGGAGATGGTG
AAGTCCACGGATTTCACACTTGTTATGGTTCCTAAACTTCTTGGCAGCGTGCCAGAGGAACTAGATGGCCTCAACATTAT
CGCCATGATTGAAACCCCTCAGGCTGCAACCAGCATTCCTCAGATTGCTGCGGATCCTAAAGTCGTTGGAATGTTCTGGG
GCGCGGAGGATCTCACACACCTCTTGGGAGGCACTCATTCTAGGTTCTTGGGTGATGAGTCCAATGAAGGCTCCTACCGA
GACACCATGAGGCTTACACGCGCCCTGATGCACCTCCAGGCGACGGCAAATGGGAAGTTCACCATTGATGCCATCCATGC
GGATTTCCACGATGAAGAGGGCCTCTATTTAGAAGCGGTCGATGCTGCGCGGACTGGTTTCGCTGGCACCGCATGCATTC
ACCCCAAGCAGATCGAGACTGTACGGAAAGCCTATAGGCCAGAGGCAGGGCAGTTGGAGTGGGCAACAAAAGTGGTGGAG
GAAGCAAAAAACCATCCAGGTGCGTTCAAACTGGATGGTCAGATGATTGATGCTCCGCTGATTTCGCAGGCGCGGATGGT
TATTTCGCGTCAGCCTGCTTGA

Upstream 100 bases:

>100_bases
ATGTACCTGACTGGTGCGCGACACTTCGCTCACTAAAGTTTTTAAAGATTTCGCTTGAAGGCAGACCATAAGGTCTGCCT
TTTCGCGTATTAATGAGTAC

Downstream 100 bases:

>100_bases
TTAGTTCAAGCGTTTTTTCGACCCGGTCGGCGGGCAGCGGCGCGCCGAGGACGGCGAGGGAGGCGTCGGCAAGCATAATT
GCGGTCTCCGGGAGGCTGTC

Product: hypothetical protein

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKRTIVRTVGPSDPHFLADVEMV
KSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIPQIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYR
DTMRLTRALMHLQATANGKFTIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIETVRKAYRPEAGQLEWATKVVE
EAKNHPGAFKLDGQMIDAPLISQARMVISRQPA

Sequences:

>Translated_273_residues
MSELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKRTIVRTVGPSDPHFLADVEMV
KSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIPQIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYR
DTMRLTRALMHLQATANGKFTIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIETVRKAYRPEAGQLEWATKVVE
EAKNHPGAFKLDGQMIDAPLISQARMVISRQPA
>Mature_272_residues
SELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKRTIVRTVGPSDPHFLADVEMVK
STDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIPQIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYRD
TMRLTRALMHLQATANGKFTIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIETVRKAYRPEAGQLEWATKVVEE
AKNHPGAFKLDGQMIDAPLISQARMVISRQPA

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=298, Percent_Identity=26.1744966442953, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87081764, Length=288, Percent_Identity=26.3888888888889, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557458, Length=304, Percent_Identity=25.9868421052632, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 29627; Mature: 29496

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKR
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHH
TIVRTVGPSDPHFLADVEMVKSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIP
HHEEECCCCCCCEEHHHHHHHCCCEEEEEHHHHHCCCHHHHCCCEEEEEEECCCHHHCCC
QIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYRDTMRLTRALMHLQATANGKF
HHCCCCEEEEEEECHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
TIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIETVRKAYRPEAGQLEWATKVVE
EEEEEECCCCCCCCCEEEEEHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHH
EAKNHPGAFKLDGQMIDAPLISQARMVISRQPA
HHHCCCCEEEECCEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKR
CCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHH
TIVRTVGPSDPHFLADVEMVKSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIP
HHEEECCCCCCCEEHHHHHHHCCCEEEEEHHHHHCCCHHHHCCCEEEEEEECCCHHHCCC
QIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYRDTMRLTRALMHLQATANGKF
HHCCCCEEEEEEECHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
TIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIETVRKAYRPEAGQLEWATKVVE
EEEEEECCCCCCCCCEEEEEHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHH
EAKNHPGAFKLDGQMIDAPLISQARMVISRQPA
HHHCCCCEEEECCEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]