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The map label for this gene is purH [H]

Identifier: 145295032

GI number: 145295032

Start: 1084529

End: 1086091

Strand: Direct

Name: purH [H]

Synonym: cgR_0976

Alternate gene names: 145295032

Gene position: 1084529-1086091 (Clockwise)

Preceding gene: 145295031

Following gene: 145295033

Centisome position: 32.72

GC content: 56.81

Gene sequence:

>1563_bases
ATGAGCGATGATCGTAAGGCAATTAAACGCGCACTAATTAGCGTGTATGACAAGACTGGCCTGGAGGATCTAGCCCAGGC
ACTTCACCGCGAGAACGTGGAAATTGTTTCCACCGGATCCACTGCGGCGAAGATTGCTGAGCTTGGTATTCCTGTTACCC
CGGTTGAGGAGCTCACCGGTTTCCCTGAGTGCCTTGAGGGCCGTGTGAAGACACTGCACCCTAAGGTTCACGCTGGCATC
TTGGCGGACACCCGCAAGGAAGACCACCTGCGTCAGCTCAAGGAACTTGAGGTCGCCCCATTCCAGCTCGTCGTGGTGAA
CCTGTACCCATTTGCTGAGACCGTTGCGTCCGGCGCCGATTTCGATGCTTGCGTTGAGCAGATCGACATCGGAGGCCCAT
CCATGGTTCGTGCTGCGGCAAAGAACCACCCATCTGTCGCTGTGGTTGTTTCACCAAACCGCTACGAGGATGTCCAGGAA
GCTTTGAAGACCGGTGGATTCTCCCGCGCGGAGCGCACCAAGTTGGCTGCTGAGGCTTTCCGCCACACCGCAACCTACGA
TGTCACCGTTGCAACCTGGATGAGCGAGCAGCTGGCTGCCGAAGATTCTGAGACTGAGTTCCCAGGTTGGATCGGCACCA
CCAACACCTTGTCCCGTAGCTTGCGTTACGGTGAGAACCCTCACCAGTCTGCAGCTTTGTACGTGGGCAACATCCGCGGA
CTTGCACAGGCTAAGCAGTTCCACGGCAAGGAAATGAGCTACAACAACTACACCGATTCTGATGCTGCATGGCGTGCAGC
GTGGGATCACGAGCGTCCTTGTGTAGCTATCATCAAGCATGCAAACCCTTGTGGCATTGCTGTCTCTGATGAGTCCATCG
CAGCGGCACACCGCGAGGCACACGCATGTGACTCTGTGTCCGCATTTGGTGGCGTCATCGCGTCCAACCGTGAAGTCAGC
GTTGAGATGGCTAACCAGGTTGCAGAGATCTTCACTGAGGTCATCATCGCTCCTTCCTACGAAGAGGGCGCTGTGGAGAT
CCTGAGCCAGAAGAAGAACATCCGTATTCTTCAGGCTGAAGCACCTGTGCGTAAGGGCTTTGAGTCCCGTGAGATCTCCG
GCGGTCTGCTTGTTCAGGAACGCGACTTGATCCACGCAGAGGGCGACAACTCCGCAAACTGGACTCTTGCTGCCGGCTCT
GCTGTTTCTCCTGAGGTTCTGAAGGACCTGGAGTTCGCGTGGACTGCAGTTCGTTCCGTGAAGTCCAACGCAATTCTGTT
GGCTAAGAACGGCGCTACCGTTGGCGTTGGCATGGGACAGGTCAACCGCGTTGACTCTGCTCGCTTGGCTGTCGACCGTG
CAGGTGCAGAGCGCGCTACCGGTTCCGTTGCTGCTTCCGATGCGTTCTTCCCATTCGCTGACGGCTTTGAGGTTCTCGCT
GAGGCTGGCATCACTGCTGTTGTGCAGCCTGGTGGATCCATTCGCGACAACGAGGTCATTGAGGCAGCCAACAAGGCTGG
CGTGACCATGTACCTGACTGGTGCGCGACACTTCGCTCACTAA

Upstream 100 bases:

>100_bases
ATCAAGCAGGTTGAGCGTAAACTCATTGTAGAAGTCCTGAACAGCGTGGAATTTTCGCGTCAGGGTGGCGTACAACTCAA
CTGGAGAGGCTAAATCCTTC

Downstream 100 bases:

>100_bases
AGTTTTTAAAGATTTCGCTTGAAGGCAGACCATAAGGTCTGCCTTTTCGCGTATTAATGAGTACATGTCTGAACTTATTT
GTGGACCAGCTATTCTCTTC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSDDRKAIKRALISVYDKTGLEDLAQALHRENVEIVSTGSTAAKIAELGIPVTPVEELTGFPECLEGRVKTLHPKVHAGI
LADTRKEDHLRQLKELEVAPFQLVVVNLYPFAETVASGADFDACVEQIDIGGPSMVRAAAKNHPSVAVVVSPNRYEDVQE
ALKTGGFSRAERTKLAAEAFRHTATYDVTVATWMSEQLAAEDSETEFPGWIGTTNTLSRSLRYGENPHQSAALYVGNIRG
LAQAKQFHGKEMSYNNYTDSDAAWRAAWDHERPCVAIIKHANPCGIAVSDESIAAAHREAHACDSVSAFGGVIASNREVS
VEMANQVAEIFTEVIIAPSYEEGAVEILSQKKNIRILQAEAPVRKGFESREISGGLLVQERDLIHAEGDNSANWTLAAGS
AVSPEVLKDLEFAWTAVRSVKSNAILLAKNGATVGVGMGQVNRVDSARLAVDRAGAERATGSVAASDAFFPFADGFEVLA
EAGITAVVQPGGSIRDNEVIEAANKAGVTMYLTGARHFAH

Sequences:

>Translated_520_residues
MSDDRKAIKRALISVYDKTGLEDLAQALHRENVEIVSTGSTAAKIAELGIPVTPVEELTGFPECLEGRVKTLHPKVHAGI
LADTRKEDHLRQLKELEVAPFQLVVVNLYPFAETVASGADFDACVEQIDIGGPSMVRAAAKNHPSVAVVVSPNRYEDVQE
ALKTGGFSRAERTKLAAEAFRHTATYDVTVATWMSEQLAAEDSETEFPGWIGTTNTLSRSLRYGENPHQSAALYVGNIRG
LAQAKQFHGKEMSYNNYTDSDAAWRAAWDHERPCVAIIKHANPCGIAVSDESIAAAHREAHACDSVSAFGGVIASNREVS
VEMANQVAEIFTEVIIAPSYEEGAVEILSQKKNIRILQAEAPVRKGFESREISGGLLVQERDLIHAEGDNSANWTLAAGS
AVSPEVLKDLEFAWTAVRSVKSNAILLAKNGATVGVGMGQVNRVDSARLAVDRAGAERATGSVAASDAFFPFADGFEVLA
EAGITAVVQPGGSIRDNEVIEAANKAGVTMYLTGARHFAH
>Mature_519_residues
SDDRKAIKRALISVYDKTGLEDLAQALHRENVEIVSTGSTAAKIAELGIPVTPVEELTGFPECLEGRVKTLHPKVHAGIL
ADTRKEDHLRQLKELEVAPFQLVVVNLYPFAETVASGADFDACVEQIDIGGPSMVRAAAKNHPSVAVVVSPNRYEDVQEA
LKTGGFSRAERTKLAAEAFRHTATYDVTVATWMSEQLAAEDSETEFPGWIGTTNTLSRSLRYGENPHQSAALYVGNIRGL
AQAKQFHGKEMSYNNYTDSDAAWRAAWDHERPCVAIIKHANPCGIAVSDESIAAAHREAHACDSVSAFGGVIASNREVSV
EMANQVAEIFTEVIIAPSYEEGAVEILSQKKNIRILQAEAPVRKGFESREISGGLLVQERDLIHAEGDNSANWTLAAGSA
VSPEVLKDLEFAWTAVRSVKSNAILLAKNGATVGVGMGQVNRVDSARLAVDRAGAERATGSVAASDAFFPFADGFEVLAE
AGITAVVQPGGSIRDNEVIEAANKAGVTMYLTGARHFAH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=608, Percent_Identity=32.5657894736842, Blast_Score=235, Evalue=9e-62,
Organism=Escherichia coli, GI1790439, Length=537, Percent_Identity=45.0651769087523, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71985564, Length=482, Percent_Identity=32.9875518672199, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71985574, Length=314, Percent_Identity=26.1146496815287, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985556, Length=141, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323056, Length=481, Percent_Identity=36.5904365904366, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6323768, Length=476, Percent_Identity=35.7142857142857, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24649832, Length=475, Percent_Identity=35.3684210526316, Blast_Score=219, Evalue=5e-57,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55772; Mature: 55641

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDRKAIKRALISVYDKTGLEDLAQALHRENVEIVSTGSTAAKIAELGIPVTPVEELTG
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCHHHHCC
FPECLEGRVKTLHPKVHAGILADTRKEDHLRQLKELEVAPFQLVVVNLYPFAETVASGAD
CHHHHCCHHHHCCCHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEEEECHHHHHHHCCCC
FDACVEQIDIGGPSMVRAAAKNHPSVAVVVSPNRYEDVQEALKTGGFSRAERTKLAAEAF
HHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
RHTATYDVTVATWMSEQLAAEDSETEFPGWIGTTNTLSRSLRYGENPHQSAALYVGNIRG
HHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECHHH
LAQAKQFHGKEMSYNNYTDSDAAWRAAWDHERPCVAIIKHANPCGIAVSDESIAAAHREA
HHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHH
HACDSVSAFGGVIASNREVSVEMANQVAEIFTEVIIAPSYEEGAVEILSQKKNIRILQAE
HHHHHHHHHCCEEECCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEC
APVRKGFESREISGGLLVQERDLIHAEGDNSANWTLAAGSAVSPEVLKDLEFAWTAVRSV
CHHHCCCCCCCCCCCEEEEECCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KSNAILLAKNGATVGVGMGQVNRVDSARLAVDRAGAERATGSVAASDAFFPFADGFEVLA
CCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCHHCCCCEEECCCCCCCCCHHHHHH
EAGITAVVQPGGSIRDNEVIEAANKAGVTMYLTGARHFAH
HCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCHHCCC
>Mature Secondary Structure 
SDDRKAIKRALISVYDKTGLEDLAQALHRENVEIVSTGSTAAKIAELGIPVTPVEELTG
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCHHHHCC
FPECLEGRVKTLHPKVHAGILADTRKEDHLRQLKELEVAPFQLVVVNLYPFAETVASGAD
CHHHHCCHHHHCCCHHHHCHHHCCCCHHHHHHHHHCCCCCEEEEEEEECHHHHHHHCCCC
FDACVEQIDIGGPSMVRAAAKNHPSVAVVVSPNRYEDVQEALKTGGFSRAERTKLAAEAF
HHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
RHTATYDVTVATWMSEQLAAEDSETEFPGWIGTTNTLSRSLRYGENPHQSAALYVGNIRG
HHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECHHH
LAQAKQFHGKEMSYNNYTDSDAAWRAAWDHERPCVAIIKHANPCGIAVSDESIAAAHREA
HHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHH
HACDSVSAFGGVIASNREVSVEMANQVAEIFTEVIIAPSYEEGAVEILSQKKNIRILQAE
HHHHHHHHHCCEEECCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEC
APVRKGFESREISGGLLVQERDLIHAEGDNSANWTLAAGSAVSPEVLKDLEFAWTAVRSV
CHHHCCCCCCCCCCCEEEEECCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KSNAILLAKNGATVGVGMGQVNRVDSARLAVDRAGAERATGSVAASDAFFPFADGFEVLA
CCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCHHCCCCEEECCCCCCCCCHHHHHH
EAGITAVVQPGGSIRDNEVIEAANKAGVTMYLTGARHFAH
HCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]