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The map label for this gene is thiI [H]

Identifier: 138896335

GI number: 138896335

Start: 2806396

End: 2807601

Strand: Reverse

Name: thiI [H]

Synonym: GTNG_2698

Alternate gene names: 138896335

Gene position: 2807601-2806396 (Counterclockwise)

Preceding gene: 138896336

Following gene: 138896334

Centisome position: 79.08

GC content: 49.59

Gene sequence:

>1206_bases
ATGAAGTATGACCGCATTTTAATCCGTTATGGGGAAATGACGACAAAAGGAAAAAACCGCAATATTTTTGTTCGACGGCT
AAAAAACAACATCGCCCGCAAACTGCAGGCGTTTCCTCGGATCAAAATTGAATATATGCGTGACCGAATGTACATTTTAT
TAAACGGTGAGCCGCATGAACCGATTATCGACAAGCTGAAAACGGTGTTTGGCATCCACTCGTTCAGCTTGGCGATGAAG
TGCGAAAACGATTTAGCGGCTATTAAAGAGACGGCGCTCGCTGCTGTCCGGCAGCTTCCATACAAAGGAAAAACGTTTAA
AGTGAGCGCCCGCCGCGTCGATAAACAGTTTCCGTACCGAAGTGATGAGCTTAATCATGAAGTTGGGGCACATATTTTGC
GGCAGACGGAAGACTTAACGGTCAATGTGCGCCAACCGGATATTGATGTGCGCATCGAAGTACGCCAAGACGGAACCTAT
GTTACGTGCCATGACATTTTCGGCGCTGGCGGCTTGCCGGTGGGGACGAGCGGCAAGGCGATGCTCATGCTCTCAGGCGG
CATCGACAGCCCGGTCGCCGGGTATTTGGCGATGAAGCGCGGTCTCGAAATCGAAGCCGTTCACTTTTTCAGTCCGCCGT
TTACAAGCGAGCGGGCAAAGCAAAAAGTGATCGATTTGGTGCGGAAACTGACCGCGTACGGTGGAAAAATCAAGTTGCAT
ATCGTACCGTTTACCGAAGTGCAGCAAGCCATTTATCAAGGGGTTCCAAACGAATATTCGCTGATTTCCACTCGGAGGGC
AATGCTAAAAATTACAGACGCCTTGCGCCGCCGCCAGCGGGGGTTGGCGATCGTCACCGGTGAAAGCCTCGGCCAAGTTG
CGAGCCAAACGCTTGAAAGCATGTACGTGATTAACGAAGTGACCAACACACCGGTTTTGCGCCCGCTCATTTCGATGGAT
AAAATGGAAATCATCGAGATTGCTAAGCAAATCGACACCCATGATATTTCCATTCTTCCATATGAAGACTGTTGCACCAT
TTTTACGCCGCGGGCGCCAAAAACAAAGCCGAAAAAAGAGAAAGTGCTTCAGCACGAGAGCCAGCTTGATCTCGCTCCGC
TTCTTGAAAAAGCGATCAATGAGACGGAAACCATCGTGATTGATGAAGAAGCTGGACAGGCTGACGAGTTCACTGCGCTG
TTTTAA

Upstream 100 bases:

>100_bases
AGCTTATCGTTTGACAACACAAGCGAAGAAATCGCGCCGGCAGTGGCTGCCATGAAACAAGCGATCAAAACGTTAAGCGA
GGTAACGGGATAACATGGAA

Downstream 100 bases:

>100_bases
ATCTCAAAATTTACCAAACATGTTGTTGCATATTGCTGTCTGTTTTCACACATTCTATACGTACAAAGGAGGTGAAAACA
GATGGCACGCAACAACAACA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKYDRILIRYGEMTTKGKNRNIFVRRLKNNIARKLQAFPRIKIEYMRDRMYILLNGEPHEPIIDKLKTVFGIHSFSLAMK
CENDLAAIKETALAAVRQLPYKGKTFKVSARRVDKQFPYRSDELNHEVGAHILRQTEDLTVNVRQPDIDVRIEVRQDGTY
VTCHDIFGAGGLPVGTSGKAMLMLSGGIDSPVAGYLAMKRGLEIEAVHFFSPPFTSERAKQKVIDLVRKLTAYGGKIKLH
IVPFTEVQQAIYQGVPNEYSLISTRRAMLKITDALRRRQRGLAIVTGESLGQVASQTLESMYVINEVTNTPVLRPLISMD
KMEIIEIAKQIDTHDISILPYEDCCTIFTPRAPKTKPKKEKVLQHESQLDLAPLLEKAINETETIVIDEEAGQADEFTAL
F

Sequences:

>Translated_401_residues
MKYDRILIRYGEMTTKGKNRNIFVRRLKNNIARKLQAFPRIKIEYMRDRMYILLNGEPHEPIIDKLKTVFGIHSFSLAMK
CENDLAAIKETALAAVRQLPYKGKTFKVSARRVDKQFPYRSDELNHEVGAHILRQTEDLTVNVRQPDIDVRIEVRQDGTY
VTCHDIFGAGGLPVGTSGKAMLMLSGGIDSPVAGYLAMKRGLEIEAVHFFSPPFTSERAKQKVIDLVRKLTAYGGKIKLH
IVPFTEVQQAIYQGVPNEYSLISTRRAMLKITDALRRRQRGLAIVTGESLGQVASQTLESMYVINEVTNTPVLRPLISMD
KMEIIEIAKQIDTHDISILPYEDCCTIFTPRAPKTKPKKEKVLQHESQLDLAPLLEKAINETETIVIDEEAGQADEFTAL
F
>Mature_401_residues
MKYDRILIRYGEMTTKGKNRNIFVRRLKNNIARKLQAFPRIKIEYMRDRMYILLNGEPHEPIIDKLKTVFGIHSFSLAMK
CENDLAAIKETALAAVRQLPYKGKTFKVSARRVDKQFPYRSDELNHEVGAHILRQTEDLTVNVRQPDIDVRIEVRQDGTY
VTCHDIFGAGGLPVGTSGKAMLMLSGGIDSPVAGYLAMKRGLEIEAVHFFSPPFTSERAKQKVIDLVRKLTAYGGKIKLH
IVPFTEVQQAIYQGVPNEYSLISTRRAMLKITDALRRRQRGLAIVTGESLGQVASQTLESMYVINEVTNTPVLRPLISMD
KMEIIEIAKQIDTHDISILPYEDCCTIFTPRAPKTKPKKEKVLQHESQLDLAPLLEKAINETETIVIDEEAGQADEFTAL
F

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=398, Percent_Identity=29.1457286432161, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45401; Mature: 45401

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDRILIRYGEMTTKGKNRNIFVRRLKNNIARKLQAFPRIKIEYMRDRMYILLNGEPHE
CCCCEEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEECCCCCC
PIIDKLKTVFGIHSFSLAMKCENDLAAIKETALAAVRQLPYKGKTFKVSARRVDKQFPYR
HHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCC
SDELNHEVGAHILRQTEDLTVNVRQPDIDVRIEVRQDGTYVTCHDIFGAGGLPVGTSGKA
CCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCEEEEEHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLAMKRGLEIEAVHFFSPPFTSERAKQKVIDLVRKLTAYGGKIKLH
EEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
IVPFTEVQQAIYQGVPNEYSLISTRRAMLKITDALRRRQRGLAIVTGESLGQVASQTLES
EECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
MYVINEVTNTPVLRPLISMDKMEIIEIAKQIDTHDISILPYEDCCTIFTPRAPKTKPKKE
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEEHHCCCCEECCCCCCCCCHHH
KVLQHESQLDLAPLLEKAINETETIVIDEEAGQADEFTALF
HHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECC
>Mature Secondary Structure
MKYDRILIRYGEMTTKGKNRNIFVRRLKNNIARKLQAFPRIKIEYMRDRMYILLNGEPHE
CCCCEEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEECCCCCC
PIIDKLKTVFGIHSFSLAMKCENDLAAIKETALAAVRQLPYKGKTFKVSARRVDKQFPYR
HHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCC
SDELNHEVGAHILRQTEDLTVNVRQPDIDVRIEVRQDGTYVTCHDIFGAGGLPVGTSGKA
CCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCEEEEEHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLAMKRGLEIEAVHFFSPPFTSERAKQKVIDLVRKLTAYGGKIKLH
EEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
IVPFTEVQQAIYQGVPNEYSLISTRRAMLKITDALRRRQRGLAIVTGESLGQVASQTLES
EECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
MYVINEVTNTPVLRPLISMDKMEIIEIAKQIDTHDISILPYEDCCTIFTPRAPKTKPKKE
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEEHHCCCCEECCCCCCCCCHHH
KVLQHESQLDLAPLLEKAINETETIVIDEEAGQADEFTALF
HHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA