| Definition | Geobacillus thermodenitrificans NG80-2 chromosome, complete genome. |
|---|---|
| Accession | NC_009328 |
| Length | 3,550,319 |
Click here to switch to the map view.
The map label for this gene is polA [H]
Identifier: 138896291
GI number: 138896291
Start: 2760482
End: 2763118
Strand: Reverse
Name: polA [H]
Synonym: GTNG_2654
Alternate gene names: 138896291
Gene position: 2763118-2760482 (Counterclockwise)
Preceding gene: 138896292
Following gene: 138896290
Centisome position: 77.83
GC content: 50.55
Gene sequence:
>2637_bases ATGAGATTGAAGAAAAAACTCGTCTTAATTGATGGCAACAGTGTGGCATACCGCGCCTTTTTTGCCTTGCCACTTTTGCA TAACGACAAAGGCATTCATACGAATGCGGTTTACGGGTTTACGATGATGTTGAACAAAATTTTGGCGGAAGAACAACCGA CCCATTTACTTGTAGCGTTTGACGCCGGAAAAACGACGTTCCGGCATGAAACGTTTCAAGAGTATAAAGGCGGACGGCAA CAAACTCCCCCGGAACTGTCCGAGCAGTTTCCGCTGTTGCGCGAGCTATTAAAAGCGTACCGCATTCCCGCTTATGAACT TGATCATTACGAAGCGGACGATATTATCGGGACGCTCGCTGCCCGCGCTGAGCAAGAAGGGTTTGAAGTGAAAATCATTT CCGGCGACCGCGATTTAACCCAGCTCGCCTCCCGTCATGTGACGGTCGATATTACGAAAAAAGGGATTACCGACATTGAG CCGTATACGCCAGAGACCGTTCGCGAAAAATACGGCCTGACTCCGGAGCAAATAGTGGATTTAAAAGGATTGATGGGCGA TAAATCCGACAACATCCCGGGCGTGCCCGGCATCGGGGAAAAAACGGCGGTCAAGCTGCTGAAGCAATTTGGTACGGTGG AAAATGTGCTCGCATCGATTGATGAGGTGAAAGGGGAAAAACTGAAAGAAAACTTGCGCCAACACCGGGATTTAGCTCTC TTGAGCAAACAGCTGGCGTCCATTTGCCGCGACGCCCCGGTTGAGCTGTCGTTAGATGACATTGTCTACGAAGGACAAGA CCGCGAAAAAGTCATCGCGTTATTTAAAGAACTCGGGTTTCAGTCGTTCTTGGAAAAAATGGCCGCGCCGGCAGCCGAAG GGGAGAAACCGCTTGAGGAGATGGAGTTTGCCATCGTTGACGTCATTACCGAAGAGATGCTTGCCGACAAGGCAGCGCTT GTCGTTGAGGTGATGGAAGAAAACTACCACGATGCCCCGATTGTCGGAATCGCACTAGTGAACGAGCATGGGCGATTTTT TATGCGCCCGGAGACCGCGCTGGCTGATTCGCAATTTTTAGCATGGCTTGCCGATGAAACGAAGAAAAAAAGCATGTTTG ACGCCAAGCGGGCAGTCGTTGCCTTAAAGTGGAAAGGAATTGAGCTTCGCGGCGTCGCCTTTGATTTATTGCTCGCTGCC TATTTGCTCAATCCGGCTCAAGATGCCGGCGATATCGCTGCGGTGGCGAAAATGAAACAATATGAAGCGGTGCGGTCGGA TGAAGCGGTCTATGGCAAAGGCGTCAAGCGGTCGCTGCCGGACGAACAGACGCTTGCTGAGCATCTCGTTCGCAAAGCGG CAGCCATTTGGGCGCTTGAGCAGCCGTTTATGGACGATTTGCGGAACAACGAACAAGATCAATTATTAACGAAGCTTGAG CAGCCGCTGGCGGCGATTTTGGCTGAAATGGAATTCACTGGGGTGAACGTGGATACAAAGCGGCTTGAACAGATGGGTTC GGAGCTCGCCGAACAACTGCGTGCCATCGAGCAGCGCATTTACGAGCTAGCCGGCCAAGAGTTCAACATTAACTCACCAA AACAGCTCGGAGTCATTTTATTTGAAAAGCTGCAGCTACCGGTGCTGAAGAAGACGAAAACAGGCTATTCGACTTCGGCT GATGTGCTTGAGAAGCTTGCGCCGCATCATGAAATCGTCGAAAACATTTTGCATTACCGCCAGCTTGGCAAACTGCAATC AACGTATATTGAAGGATTGTTGAAAGTTGTGCGCCCTGATACCGGCAAAGTGCATACGATGTTCAACCAAGCGCTGACGC AAACTGGGCGGCTCAGCTCGGCCGAGCCGAACTTGCAAAACATTCCGATTCGGCTCGAAGAGGGGCGGAAAATCCGCCAA GCGTTCGTCCCGTCAGAGCCGGACTGGCTCATTTTCGCCGCCGATTACTCACAAATTGAATTGCGCGTCCTCGCCCATAT CGCCGATGACGACAATCTAATTGAAGCGTTCCAACGCGATTTGGATATTCACACAAAAACGGCGATGGACATTTTCCATG TGAGCGAAGAGGAAGTCACGGCCAACATGCGCCGCCAGGCAAAGGCCGTTAACTTCGGTATCGTTTACGGAATTAGCGAT TACGGATTGGCGCAAAACTTGAACATTACGCGCAAAGAAGCTGCCGAATTTATCGAACGTTACTTCGCCAGCTTTCCGGG CGTAAAGCAGTATATGGAAAACATTGTGCAAGAAGCGAAACAGAAAGGATATGTGACAACGCTGTTGCATCGGCGCCGCT ATTTGCCTGATATTACAAGCCGCAATTTCAACGTCCGCAGTTTTGCAGAGCGGACGGCCATGAACACGCCAATTCAAGGA AGCGCCGCTGACATTATTAAAAAAGCGATGATTGATTTAGCGGCACGGCTGAAAGAAGAGCAGCTTCAGGCTCGTCTTTT GCTGCAAGTGCATGACGAGCTCATTTTGGAAGCGCCAAAAGAGGAAATTGAGCGATTATGTGAGCTTGTTCCGGAAGTGA TGGAGCAGGCCGTTACGCTCCGCGTGCCGCTGAAAGTCGACTACCATTACGGCCCAACATGGTATGATGCCAAATAA
Upstream 100 bases:
>100_bases GTGTTTACGATCCATTTTCCAAAGCCGGAGCGGTAGCCGGCTTCTTTTTTATGAGAAGCGGTTTGCATGGTACAATAGAA CAAGGAGCGTTCGAGGAGGG
Downstream 100 bases:
>100_bases GAGGTACGGGGGCGGAAACGGGCCGGCCAATCATAGAAAAAGAACGTCTCCCAGCCGGGCTGATGCGAATGGGAAAAAGC TTTTGCCCGGTTGCGGGGCA
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 878; Mature: 878
Protein sequence:
>878_residues MRLKKKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHLLVAFDAGKTTFRHETFQEYKGGRQ QTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTLAARAEQEGFEVKIISGDRDLTQLASRHVTVDITKKGITDIE PYTPETVREKYGLTPEQIVDLKGLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEVKGEKLKENLRQHRDLAL LSKQLASICRDAPVELSLDDIVYEGQDREKVIALFKELGFQSFLEKMAAPAAEGEKPLEEMEFAIVDVITEEMLADKAAL VVEVMEENYHDAPIVGIALVNEHGRFFMRPETALADSQFLAWLADETKKKSMFDAKRAVVALKWKGIELRGVAFDLLLAA YLLNPAQDAGDIAAVAKMKQYEAVRSDEAVYGKGVKRSLPDEQTLAEHLVRKAAAIWALEQPFMDDLRNNEQDQLLTKLE QPLAAILAEMEFTGVNVDTKRLEQMGSELAEQLRAIEQRIYELAGQEFNINSPKQLGVILFEKLQLPVLKKTKTGYSTSA DVLEKLAPHHEIVENILHYRQLGKLQSTYIEGLLKVVRPDTGKVHTMFNQALTQTGRLSSAEPNLQNIPIRLEEGRKIRQ AFVPSEPDWLIFAADYSQIELRVLAHIADDDNLIEAFQRDLDIHTKTAMDIFHVSEEEVTANMRRQAKAVNFGIVYGISD YGLAQNLNITRKEAAEFIERYFASFPGVKQYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQG SAADIIKKAMIDLAARLKEEQLQARLLLQVHDELILEAPKEEIERLCELVPEVMEQAVTLRVPLKVDYHYGPTWYDAK
Sequences:
>Translated_878_residues MRLKKKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHLLVAFDAGKTTFRHETFQEYKGGRQ QTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTLAARAEQEGFEVKIISGDRDLTQLASRHVTVDITKKGITDIE PYTPETVREKYGLTPEQIVDLKGLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEVKGEKLKENLRQHRDLAL LSKQLASICRDAPVELSLDDIVYEGQDREKVIALFKELGFQSFLEKMAAPAAEGEKPLEEMEFAIVDVITEEMLADKAAL VVEVMEENYHDAPIVGIALVNEHGRFFMRPETALADSQFLAWLADETKKKSMFDAKRAVVALKWKGIELRGVAFDLLLAA YLLNPAQDAGDIAAVAKMKQYEAVRSDEAVYGKGVKRSLPDEQTLAEHLVRKAAAIWALEQPFMDDLRNNEQDQLLTKLE QPLAAILAEMEFTGVNVDTKRLEQMGSELAEQLRAIEQRIYELAGQEFNINSPKQLGVILFEKLQLPVLKKTKTGYSTSA DVLEKLAPHHEIVENILHYRQLGKLQSTYIEGLLKVVRPDTGKVHTMFNQALTQTGRLSSAEPNLQNIPIRLEEGRKIRQ AFVPSEPDWLIFAADYSQIELRVLAHIADDDNLIEAFQRDLDIHTKTAMDIFHVSEEEVTANMRRQAKAVNFGIVYGISD YGLAQNLNITRKEAAEFIERYFASFPGVKQYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQG SAADIIKKAMIDLAARLKEEQLQARLLLQVHDELILEAPKEEIERLCELVPEVMEQAVTLRVPLKVDYHYGPTWYDAK >Mature_878_residues MRLKKKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHLLVAFDAGKTTFRHETFQEYKGGRQ QTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTLAARAEQEGFEVKIISGDRDLTQLASRHVTVDITKKGITDIE PYTPETVREKYGLTPEQIVDLKGLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEVKGEKLKENLRQHRDLAL LSKQLASICRDAPVELSLDDIVYEGQDREKVIALFKELGFQSFLEKMAAPAAEGEKPLEEMEFAIVDVITEEMLADKAAL VVEVMEENYHDAPIVGIALVNEHGRFFMRPETALADSQFLAWLADETKKKSMFDAKRAVVALKWKGIELRGVAFDLLLAA YLLNPAQDAGDIAAVAKMKQYEAVRSDEAVYGKGVKRSLPDEQTLAEHLVRKAAAIWALEQPFMDDLRNNEQDQLLTKLE QPLAAILAEMEFTGVNVDTKRLEQMGSELAEQLRAIEQRIYELAGQEFNINSPKQLGVILFEKLQLPVLKKTKTGYSTSA DVLEKLAPHHEIVENILHYRQLGKLQSTYIEGLLKVVRPDTGKVHTMFNQALTQTGRLSSAEPNLQNIPIRLEEGRKIRQ AFVPSEPDWLIFAADYSQIELRVLAHIADDDNLIEAFQRDLDIHTKTAMDIFHVSEEEVTANMRRQAKAVNFGIVYGISD YGLAQNLNITRKEAAEFIERYFASFPGVKQYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQG SAADIIKKAMIDLAARLKEEQLQARLLLQVHDELILEAPKEEIERLCELVPEVMEQAVTLRVPLKVDYHYGPTWYDAK
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=517, Percent_Identity=33.0754352030948, Blast_Score=231, Evalue=2e-60, Organism=Homo sapiens, GI139394648, Length=524, Percent_Identity=29.7709923664122, Blast_Score=213, Evalue=7e-55, Organism=Escherichia coli, GI1790294, Length=922, Percent_Identity=39.8047722342733, Blast_Score=579, Evalue=1e-166, Organism=Escherichia coli, GI226510970, Length=221, Percent_Identity=31.6742081447964, Blast_Score=125, Evalue=1e-29, Organism=Caenorhabditis elegans, GI86563272, Length=445, Percent_Identity=29.6629213483146, Blast_Score=195, Evalue=8e-50, Organism=Drosophila melanogaster, GI17933644, Length=414, Percent_Identity=32.8502415458937, Blast_Score=228, Evalue=1e-59,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 99279; Mature: 99279
Theoretical pI: Translated: 5.19; Mature: 5.19
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLKKKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHLLVAF CCCCCEEEEEECCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE DAGKTTFRHETFQEYKGGRQQTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTLA ECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHH ARAEQEGFEVKIISGDRDLTQLASRHVTVDITKKGITDIEPYTPETVREKYGLTPEQIVD HHHHCCCCEEEEECCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHH LKGLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEVKGEKLKENLRQHRDLAL HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH LSKQLASICRDAPVELSLDDIVYEGQDREKVIALFKELGFQSFLEKMAAPAAEGEKPLEE HHHHHHHHHCCCCCCEEHHHHEECCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHH MEFAIVDVITEEMLADKAALVVEVMEENYHDAPIVGIALVNEHGRFFMRPETALADSQFL HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHHH AWLADETKKKSMFDAKRAVVALKWKGIELRGVAFDLLLAAYLLNPAQDAGDIAAVAKMKQ HHHHHHHHHHHHHHHHHHEEEEEECCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH YEAVRSDEAVYGKGVKRSLPDEQTLAEHLVRKAAAIWALEQPFMDDLRNNEQDQLLTKLE HHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHH QPLAAILAEMEFTGVNVDTKRLEQMGSELAEQLRAIEQRIYELAGQEFNINSPKQLGVIL HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH FEKLQLPVLKKTKTGYSTSADVLEKLAPHHEIVENILHYRQLGKLQSTYIEGLLKVVRPD HHHHCCCHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC TGKVHTMFNQALTQTGRLSSAEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIE CCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCEEEEECCCCHHH LRVLAHIADDDNLIEAFQRDLDIHTKTAMDIFHVSEEEVTANMRRQAKAVNFGIVYGISD HHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCC YGLAQNLNITRKEAAEFIERYFASFPGVKQYMENIVQEAKQKGYVTTLLHRRRYLPDITS CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC RNFNVRSFAERTAMNTPIQGSAADIIKKAMIDLAARLKEEQLQARLLLQVHDELILEAPK CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH EEIERLCELVPEVMEQAVTLRVPLKVDYHYGPTWYDAK HHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCEECCCC >Mature Secondary Structure MRLKKKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHLLVAF CCCCCEEEEEECCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE DAGKTTFRHETFQEYKGGRQQTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTLA ECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHH ARAEQEGFEVKIISGDRDLTQLASRHVTVDITKKGITDIEPYTPETVREKYGLTPEQIVD HHHHCCCCEEEEECCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHH LKGLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEVKGEKLKENLRQHRDLAL HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH LSKQLASICRDAPVELSLDDIVYEGQDREKVIALFKELGFQSFLEKMAAPAAEGEKPLEE HHHHHHHHHCCCCCCEEHHHHEECCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHH MEFAIVDVITEEMLADKAALVVEVMEENYHDAPIVGIALVNEHGRFFMRPETALADSQFL HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHHH AWLADETKKKSMFDAKRAVVALKWKGIELRGVAFDLLLAAYLLNPAQDAGDIAAVAKMKQ HHHHHHHHHHHHHHHHHHEEEEEECCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH YEAVRSDEAVYGKGVKRSLPDEQTLAEHLVRKAAAIWALEQPFMDDLRNNEQDQLLTKLE HHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHH QPLAAILAEMEFTGVNVDTKRLEQMGSELAEQLRAIEQRIYELAGQEFNINSPKQLGVIL HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH FEKLQLPVLKKTKTGYSTSADVLEKLAPHHEIVENILHYRQLGKLQSTYIEGLLKVVRPD HHHHCCCHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC TGKVHTMFNQALTQTGRLSSAEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIE CCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCEEEEECCCCHHH LRVLAHIADDDNLIEAFQRDLDIHTKTAMDIFHVSEEEVTANMRRQAKAVNFGIVYGISD HHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCC YGLAQNLNITRKEAAEFIERYFASFPGVKQYMENIVQEAKQKGYVTTLLHRRRYLPDITS CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC RNFNVRSFAERTAMNTPIQGSAADIIKKAMIDLAARLKEEQLQARLLLQVHDELILEAPK CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH EEIERLCELVPEVMEQAVTLRVPLKVDYHYGPTWYDAK HHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCEECCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7557480; 9016716; 9440698 [H]