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The map label for this gene is ansA [H]

Identifier: 138895810

GI number: 138895810

Start: 2296812

End: 2297783

Strand: Reverse

Name: ansA [H]

Synonym: GTNG_2169

Alternate gene names: 138895810

Gene position: 2297783-2296812 (Counterclockwise)

Preceding gene: 138895811

Following gene: 138895809

Centisome position: 64.72

GC content: 53.7

Gene sequence:

>972_bases
GTGACGAAACGGAAAGTAGTGCTGCTGACGACTGGCGGCACGATCGCCAGCAAACGGAATGAACGCTCCGGCCGCTTGAA
TGCCGGGGCGTTAAGTGGCGAAGAGCTTGCCGCGATGTGCCATTTGCCGAAAGAGATCGAGGTCAAAGTCGAAAGCGTGT
TCCAGTTGCCGAGCATGCATTTGACATTTGCGAATTGGCTTGAGCTAAAAAAACGGATTGAGGCGCATTTCGCCGATCCG
TCCGTCGATGGTATCGTCGTCACCCACGGGACTGATGCGCTTGAGGAGACGGCGTACTTTCTTGATTTGACTGTGAATGA
CCCGCGAACGATCGTCGTCACCGGCTCGCAGCGGGCTCCGGCCGATTTGGGCAGCGATGTGTATATCAACATCCGCCATG
CCATTTACGCTGCGTGCGCTGAGTCGCTGCGAGGAGCGGGAACAGTCGTCGTGTTCAATGAACGCATTTTTGCCGCCAAA
TACGTGAAAAAAGAACATGCTTCGAACATTCAAGGGTTTAACTCGTTTGGCTTCGGGTATTTAGGCATTATTGACAACGA
CGAAGTGCACGTGTACCAAAAACCGATCCGCCGTGAATATTACGAGCTCGTGCGCCCGCTTCCGCCCGTCGATATTGTGA
AATGTTATTTGGAAGCAGACGGAAAATTTATTAAAGCAGCCCGCGAAAGCGGTGTGGCCGGCATCGTGCTTGAAGGGGTC
GGTCGCGGGCAAGTGGCGCCGAACATGGTCGGTGAAATCGTCAAGGCTCTCGAAGCCGGCATCAAAGTCGTTGTGACGAC
GAGCGCTGAGGAAGGGGCGGTGTATACGACGTACGATTATTTAGGGAGCGCTTATGACTTGCATAAAAAAGGCGTGATTC
TCGGCAGCGATTATGACGCAAAAAAAGCGCGCATCAAGCTCGCCGTCGCCCTCGCCTCCAACGCGGATATCGACAACTTG
TTTGCCTATTAG

Upstream 100 bases:

>100_bases
CAGGAAGCATCGGCTCGGAAGCCGCTGTAACAACACCATTTGAGTGCGGTAGAAATTAGGCGGGCATCATGTTTAAAATC
GGAAAGCGAAGGTGACGGAT

Downstream 100 bases:

>100_bases
GTGAAGTTGCCTAGTAAATTCTCTTTTTCACTCCCTAGTTTCGTGTTACGATAGAGGTAAAAAGACAGAGAAACGGGGAT
CGATATGTTTTCGCTCATTT

Product: asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTKRKVVLLTTGGTIASKRNERSGRLNAGALSGEELAAMCHLPKEIEVKVESVFQLPSMHLTFANWLELKKRIEAHFADP
SVDGIVVTHGTDALEETAYFLDLTVNDPRTIVVTGSQRAPADLGSDVYINIRHAIYAACAESLRGAGTVVVFNERIFAAK
YVKKEHASNIQGFNSFGFGYLGIIDNDEVHVYQKPIRREYYELVRPLPPVDIVKCYLEADGKFIKAARESGVAGIVLEGV
GRGQVAPNMVGEIVKALEAGIKVVVTTSAEEGAVYTTYDYLGSAYDLHKKGVILGSDYDAKKARIKLAVALASNADIDNL
FAY

Sequences:

>Translated_323_residues
MTKRKVVLLTTGGTIASKRNERSGRLNAGALSGEELAAMCHLPKEIEVKVESVFQLPSMHLTFANWLELKKRIEAHFADP
SVDGIVVTHGTDALEETAYFLDLTVNDPRTIVVTGSQRAPADLGSDVYINIRHAIYAACAESLRGAGTVVVFNERIFAAK
YVKKEHASNIQGFNSFGFGYLGIIDNDEVHVYQKPIRREYYELVRPLPPVDIVKCYLEADGKFIKAARESGVAGIVLEGV
GRGQVAPNMVGEIVKALEAGIKVVVTTSAEEGAVYTTYDYLGSAYDLHKKGVILGSDYDAKKARIKLAVALASNADIDNL
FAY
>Mature_322_residues
TKRKVVLLTTGGTIASKRNERSGRLNAGALSGEELAAMCHLPKEIEVKVESVFQLPSMHLTFANWLELKKRIEAHFADPS
VDGIVVTHGTDALEETAYFLDLTVNDPRTIVVTGSQRAPADLGSDVYINIRHAIYAACAESLRGAGTVVVFNERIFAAKY
VKKEHASNIQGFNSFGFGYLGIIDNDEVHVYQKPIRREYYELVRPLPPVDIVKCYLEADGKFIKAARESGVAGIVLEGVG
RGQVAPNMVGEIVKALEAGIKVVVTTSAEEGAVYTTYDYLGSAYDLHKKGVILGSDYDAKKARIKLAVALASNADIDNLF
AY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=307, Percent_Identity=30.6188925081433, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788065, Length=332, Percent_Identity=28.3132530120482, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323189, Length=331, Percent_Identity=29.0030211480363, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323187, Length=331, Percent_Identity=29.0030211480363, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323186, Length=331, Percent_Identity=29.0030211480363, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323184, Length=331, Percent_Identity=29.0030211480363, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320527, Length=335, Percent_Identity=27.4626865671642, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35185; Mature: 35053

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01121 CASPASE_HIS ; PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRKVVLLTTGGTIASKRNERSGRLNAGALSGEELAAMCHLPKEIEVKVESVFQLPSMH
CCCCEEEEEECCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHEEEHHHHHCCCCCE
LTFANWLELKKRIEAHFADPSVDGIVVTHGTDALEETAYFLDLTVNDPRTIVVTGSQRAP
EHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCEEEEEEEECCCEEEEEECCCCCC
ADLGSDVYINIRHAIYAACAESLRGAGTVVVFNERIFAAKYVKKEHASNIQGFNSFGFGY
CCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEE
LGIIDNDEVHVYQKPIRREYYELVRPLPPVDIVKCYLEADGKFIKAARESGVAGIVLEGV
EEEECCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEHHHHCCCEEEEEECC
GRGQVAPNMVGEIVKALEAGIKVVVTTSAEEGAVYTTYDYLGSAYDLHKKGVILGSDYDA
CCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEHHHCCCHHHHCCCCEEEECCCCC
KKARIKLAVALASNADIDNLFAY
CCEEEEEEEEEECCCCHHHHCCC
>Mature Secondary Structure 
TKRKVVLLTTGGTIASKRNERSGRLNAGALSGEELAAMCHLPKEIEVKVESVFQLPSMH
CCCEEEEEECCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHEEEHHHHHCCCCCE
LTFANWLELKKRIEAHFADPSVDGIVVTHGTDALEETAYFLDLTVNDPRTIVVTGSQRAP
EHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCEEEEEEEECCCEEEEEECCCCCC
ADLGSDVYINIRHAIYAACAESLRGAGTVVVFNERIFAAKYVKKEHASNIQGFNSFGFGY
CCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEE
LGIIDNDEVHVYQKPIRREYYELVRPLPPVDIVKCYLEADGKFIKAARESGVAGIVLEGV
EEEECCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEHHHHCCCEEEEEECC
GRGQVAPNMVGEIVKALEAGIKVVVTTSAEEGAVYTTYDYLGSAYDLHKKGVILGSDYDA
CCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEHHHCCCHHHHCCCCEEEECCCCC
KKARIKLAVALASNADIDNLFAY
CCEEEEEEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]