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The map label for this gene is ispD

Identifier: 138893760

GI number: 138893760

Start: 100105

End: 100791

Strand: Direct

Name: ispD

Synonym: GTNG_0081

Alternate gene names: 138893760

Gene position: 100105-100791 (Clockwise)

Preceding gene: 138893759

Following gene: 138893761

Centisome position: 2.82

GC content: 52.4

Gene sequence:

>687_bases
ATGAATTACGAAGCGATCGTATTAGCGGCCGGACAAGGAAAACGGATGAACGCCGGCATGAACAAGCAATTTATTGAACT
TGGCGGCGAGCCGCTCATTGTCCGAACGCTGAAAGTGTTTGATGGTGATGAGCGATGCAACGGCATTGTGCTTGTCGTCA
ATCCAACTGAGCGAGGCCAGTTCGAACAACTTTTCGAGCGCTACCGCATTCGAAAAGTAGTGGCATTCGCCGATGGTGGA
CAGGAGCGGCAGCATAGTGTTTATAACGGGCTGCAGGCGCTGAGGAGTGGGGAGATTGTCCTCATTCACGACGGTGCCCG
CCCGTTTGTGCGTATCCGGCATTTGCATGAGCTAGCGGATGCCGCTGTTCAATATGGGGCGGCTATTCCAGCAGTGAGGG
TAAAAGACACGATCAAAAAAGTGGACGGTTTGTTTGTCGAACAGACGATTGATCGTTCCAGCTTGTGGGCGGTGCAAACG
CCACAAGCTTTTCGTCTGTCTTTGATCATGGAAGCGCATGAAGAAGCGAAAAAAGCCGGTTACCTCGGCACAGACGATGC
CAGCCTTGTTGAGCGGCTCGGCAGGCCGGTGAAAATCTTAGAAGGGGACTACCGCAATATTAAGCTGACAACTCCGGAGG
ACCTTTTATTTGCCGAGGCGATTTTAGCAAGCCGCATCGCAGAGTAG

Upstream 100 bases:

>100_bases
CTTGGTCACGAGCGTGTTACAAATATTTGCGGGGAGAATGATTTTTGCCAAACTAAAACTGCTGCAAAAGGCGCTGTAAA
AAGAACAGGGGAAACGTCTA

Downstream 100 bases:

>100_bases
ATGGGGGTGAAGAGATGTTTCGCATTGGACAAGGGTTTGACGTCCATCAATTCGTTGAAGGGCGTCCGCTCATCATTGGG
GGCGTACATATTCCGTATGA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNYEAIVLAAGQGKRMNAGMNKQFIELGGEPLIVRTLKVFDGDERCNGIVLVVNPTERGQFEQLFERYRIRKVVAFADGG
QERQHSVYNGLQALRSGEIVLIHDGARPFVRIRHLHELADAAVQYGAAIPAVRVKDTIKKVDGLFVEQTIDRSSLWAVQT
PQAFRLSLIMEAHEEAKKAGYLGTDDASLVERLGRPVKILEGDYRNIKLTTPEDLLFAEAILASRIAE

Sequences:

>Translated_228_residues
MNYEAIVLAAGQGKRMNAGMNKQFIELGGEPLIVRTLKVFDGDERCNGIVLVVNPTERGQFEQLFERYRIRKVVAFADGG
QERQHSVYNGLQALRSGEIVLIHDGARPFVRIRHLHELADAAVQYGAAIPAVRVKDTIKKVDGLFVEQTIDRSSLWAVQT
PQAFRLSLIMEAHEEAKKAGYLGTDDASLVERLGRPVKILEGDYRNIKLTTPEDLLFAEAILASRIAE
>Mature_228_residues
MNYEAIVLAAGQGKRMNAGMNKQFIELGGEPLIVRTLKVFDGDERCNGIVLVVNPTERGQFEQLFERYRIRKVVAFADGG
QERQHSVYNGLQALRSGEIVLIHDGARPFVRIRHLHELADAAVQYGAAIPAVRVKDTIKKVDGLFVEQTIDRSSLWAVQT
PQAFRLSLIMEAHEEAKKAGYLGTDDASLVERLGRPVKILEGDYRNIKLTTPEDLLFAEAILASRIAE

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=237, Percent_Identity=29.1139240506329, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=32.7510917030568, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_GEOTN (A4IJG4)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001124213.1
- ProteinModelPortal:   A4IJG4
- SMR:   A4IJG4
- STRING:   A4IJG4
- GeneID:   4965207
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_0081
- NMPDR:   fig|420246.5.peg.81
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- PhylomeDB:   A4IJG4
- ProtClustDB:   PRK00155
- BioCyc:   GTHE420246:GTNG_0081-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25427; Mature: 25427

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEAIVLAAGQGKRMNAGMNKQFIELGGEPLIVRTLKVFDGDERCNGIVLVVNPTERGQ
CCCEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCH
FEQLFERYRIRKVVAFADGGQERQHSVYNGLQALRSGEIVLIHDGARPFVRIRHLHELAD
HHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
AAVQYGAAIPAVRVKDTIKKVDGLFVEQTIDRSSLWAVQTPQAFRLSLIMEAHEEAKKAG
HHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
YLGTDDASLVERLGRPVKILEGDYRNIKLTTPEDLLFAEAILASRIAE
CCCCCCHHHHHHCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYEAIVLAAGQGKRMNAGMNKQFIELGGEPLIVRTLKVFDGDERCNGIVLVVNPTERGQ
CCCEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCH
FEQLFERYRIRKVVAFADGGQERQHSVYNGLQALRSGEIVLIHDGARPFVRIRHLHELAD
HHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
AAVQYGAAIPAVRVKDTIKKVDGLFVEQTIDRSSLWAVQTPQAFRLSLIMEAHEEAKKAG
HHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
YLGTDDASLVERLGRPVKILEGDYRNIKLTTPEDLLFAEAILASRIAE
CCCCCCHHHHHHCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA