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The map label for this gene is ispF

Identifier: 138893761

GI number: 138893761

Start: 100806

End: 101288

Strand: Direct

Name: ispF

Synonym: GTNG_0082

Alternate gene names: 138893761

Gene position: 100806-101288 (Clockwise)

Preceding gene: 138893760

Following gene: 138893762

Centisome position: 2.84

GC content: 53.83

Gene sequence:

>483_bases
ATGTTTCGCATTGGACAAGGGTTTGACGTCCATCAATTCGTTGAAGGGCGTCCGCTCATCATTGGGGGCGTACATATTCC
GTATGAGAAAGGGCTGCTTGGCCATTCCGATGCCGATGTGCTGCTTCACGCGGTAGCGGATGCATGCCTTGGAGCGATTG
GCGCTGGCGATATTGGTCGGCATTTTCCGGATACGGATCCGCGCTATAAAGACGCTGATTCAGCCGAGTTGCTTGCTCAC
GTATGGTCGCTCGTCCGGCAAGAAGGGTATGTGTTAGTGAACGCAGACTGCACCATTATCGCACAAAAGCCGAAGATGGC
GCCGTACATTGAGGAGATGAAGAAGGTGATCGCCCGGTTGCTCGAAGCCGAACGTTCGCAAGTCAATGTGAAGGCGACGA
CGACAGAAAAGCTTGGGTTCACTGGGCGCGAAGAAGGCGTTGCTGCTCAAGTGGTTGTGTTGCTGCAAAAGAGCGAGGCA
TAA

Upstream 100 bases:

>100_bases
AAGGGGACTACCGCAATATTAAGCTGACAACTCCGGAGGACCTTTTATTTGCCGAGGCGATTTTAGCAAGCCGCATCGCA
GAGTAGATGGGGGTGAAGAG

Downstream 100 bases:

>100_bases
TGTTTGGCTTTGTTATGATCGCTTGAGAAGCAGTGGTACAATGGGAAGCATTCCGTTCTCCGTTTTTTGCTGGGGGACGT
TTACGTGTTGTGCGGAAGAG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MFRIGQGFDVHQFVEGRPLIIGGVHIPYEKGLLGHSDADVLLHAVADACLGAIGAGDIGRHFPDTDPRYKDADSAELLAH
VWSLVRQEGYVLVNADCTIIAQKPKMAPYIEEMKKVIARLLEAERSQVNVKATTTEKLGFTGREEGVAAQVVVLLQKSEA

Sequences:

>Translated_160_residues
MFRIGQGFDVHQFVEGRPLIIGGVHIPYEKGLLGHSDADVLLHAVADACLGAIGAGDIGRHFPDTDPRYKDADSAELLAH
VWSLVRQEGYVLVNADCTIIAQKPKMAPYIEEMKKVIARLLEAERSQVNVKATTTEKLGFTGREEGVAAQVVVLLQKSEA
>Mature_160_residues
MFRIGQGFDVHQFVEGRPLIIGGVHIPYEKGLLGHSDADVLLHAVADACLGAIGAGDIGRHFPDTDPRYKDADSAELLAH
VWSLVRQEGYVLVNADCTIIAQKPKMAPYIEEMKKVIARLLEAERSQVNVKATTTEKLGFTGREEGVAAQVVVLLQKSEA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=61.1464968152866, Blast_Score=177, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_GEOTN (A4IJG5)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001124214.1
- ProteinModelPortal:   A4IJG5
- SMR:   A4IJG5
- STRING:   A4IJG5
- GeneID:   4965208
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_0082
- NMPDR:   fig|420246.5.peg.82
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   A4IJG5
- ProtClustDB:   PRK00084
- BioCyc:   GTHE420246:GTNG_0082-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17419; Mature: 17419

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIGQGFDVHQFVEGRPLIIGGVHIPYEKGLLGHSDADVLLHAVADACLGAIGAGDIGR
CCCCCCCCCHHHHHCCCCEEEECEECCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
HFPDTDPRYKDADSAELLAHVWSLVRQEGYVLVNADCTIIAQKPKMAPYIEEMKKVIARL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHH
LEAERSQVNVKATTTEKLGFTGREEGVAAQVVVLLQKSEA
HHHHHHHEEEEEECHHHCCCCCCCCCHHHHHHEEEECCCC
>Mature Secondary Structure
MFRIGQGFDVHQFVEGRPLIIGGVHIPYEKGLLGHSDADVLLHAVADACLGAIGAGDIGR
CCCCCCCCCHHHHHCCCCEEEECEECCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
HFPDTDPRYKDADSAELLAHVWSLVRQEGYVLVNADCTIIAQKPKMAPYIEEMKKVIARL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHH
LEAERSQVNVKATTTEKLGFTGREEGVAAQVVVLLQKSEA
HHHHHHHEEEEEECHHHCCCCCCCCCHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA