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The map label for this gene is tilS

Identifier: 134301750

GI number: 134301750

Start: 694833

End: 696029

Strand: Reverse

Name: tilS

Synonym: FTW_0715

Alternate gene names: 134301750

Gene position: 696029-694833 (Counterclockwise)

Preceding gene: 134301751

Following gene: 134301748

Centisome position: 36.66

GC content: 30.41

Gene sequence:

>1197_bases
ATGTCTATAAGCAAATCTCTTGTTTTAAATGAAATAAAAAAATTCTCGCCTTCTCATATTATTATTGGTTATAGTGGTGG
CGTTGACTCAAGTGTTTTACTAAATATTAGCAAAGAATTAGATATTCCACTGATTGCAATTTATATTAATCATAATCTTC
ATCGTGACTCATTAAAATGGCAAATTCATTGTCAACAAACTTGTCAAAAATATAATCTACAATTTATTAGCCACTCATTA
GATAAAGTTCCTAAGGGTGAGAGTTTTGAGGCTTGGGCAAGTAAACAAAGAATGGCATTTTTTCAAAAAATAATGCAGCA
GTACTCTAAGCCACTACTTCTATTAGGACATCACCAAGATGATCAAGCCGAGACATTCTTAATTCAAGCTATACGTGGCT
CGGGATTAGCAGGCTTAGCAGGTATCCCACATTATAAGGAACTTCATCATGGAGGTGTATTACGACCATTATTAAAATAT
AGTAAAATTGAGATTGAAGGGTTTGCTAAACTAAATAATATTTCATATATATATGATGATAGTAATGAAGATATAAAATA
CCGCAGAAACTTAATTCGCAATCAAATAATCCCGATACTACAGCAAGTAAATCCAAATATCAGTCAAACCCTCTCTCGTA
GTGCAAATATTTGTGCAGAAAGTAATAATATTTTGCAAAAGCTCCTTACAGAAAGATTACAATCGATATCTCAAGATACT
AATCTAATAATTAGCGAGTTAATAAAATTAGATGATGATATTCAAAAAAATCTTCTGCATCTTTGGTTTAAACAAAATAC
TCAACAAAGTCTTAAAAGTAAGCAAATAAAAGAGTTACATCTAGCTGTCAATAACCCCTCAACAGGTTGGCAAATCGATA
TAAGTAACTATTATCAAATTCACATCCAATATAATCAGCTTATAATAAAGTATCCAACAACTATCAATGATATAAGCAAA
GAAGATATCATAAGTTGGCTTAGCAAAAATCTTAATGAAGAAATCGATCTAACCAAAATAGTAATTCGTGATAGAAAGCC
TGATGATAAATGCAAATATCGTGGCAGAAATAAGCCTAATAAACTCAAAATACTCTTTCAAGAACTACAAATTCCTACCA
CTGAAAGATCAAAAGCAAAAATAATTTTAAAAGATCAGCAAATAATCGCTGTATATCCTTTTTTTATTTGTGGGTAA

Upstream 100 bases:

>100_bases
TGTTGTTATGGATATTAAATCAAGCTCAGATGAGGCTAAAGAACTTATTGATGAATTCAAAAAAGTTAAAGCTACCATAA
AAGCCCGTTATTTAGTATAA

Downstream 100 bases:

>100_bases
TTTTTAACTACTTTAATTTCTTTATGATTAAAGCAACAATATACTATAGTATAATTGTCACTGCCAAGATAGCCATGATA
TAGTTAATCCGAAAGATATT

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKWQIHCQQTCQKYNLQFISHSL
DKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQDDQAETFLIQAIRGSGLAGLAGIPHYKELHHGGVLRPLLKY
SKIEIEGFAKLNNISYIYDDSNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQIHIQYNQLIIKYPTTINDISK
EDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPNKLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG

Sequences:

>Translated_398_residues
MSISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKWQIHCQQTCQKYNLQFISHSL
DKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQDDQAETFLIQAIRGSGLAGLAGIPHYKELHHGGVLRPLLKY
SKIEIEGFAKLNNISYIYDDSNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQIHIQYNQLIIKYPTTINDISK
EDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPNKLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG
>Mature_397_residues
SISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKWQIHCQQTCQKYNLQFISHSLD
KVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQDDQAETFLIQAIRGSGLAGLAGIPHYKELHHGGVLRPLLKYS
KIEIEGFAKLNNISYIYDDSNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDTN
LIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQIHIQYNQLIIKYPTTINDISKE
DIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPNKLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=420, Percent_Identity=27.3809523809524, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_FRAT1 (Q14H05)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_667321.1
- ProteinModelPortal:   Q14H05
- SMR:   Q14H05
- STRING:   Q14H05
- GeneID:   4199665
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF1231
- eggNOG:   COG0037
- HOGENOM:   HBG482458
- OMA:   HVECKKI
- PhylomeDB:   Q14H05
- ProtClustDB:   CLSK934912
- BioCyc:   FTUL393115:FTF1231-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 46024; Mature: 45893

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKW
CCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEE
QIHCQQTCQKYNLQFISHSLDKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQD
EEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
DQAETFLIQAIRGSGLAGLAGIPHYKELHHGGVLRPLLKYSKIEIEGFAKLNNISYIYDD
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHEEEEEEEEECCEEEEEEC
SNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCH
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQI
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCCCCCEEEEECCEEEE
HIQYNQLIIKYPTTINDISKEDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPN
EEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHCCCCCCCH
KLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG
HHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECEEECC
>Mature Secondary Structure 
SISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKW
CCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEE
QIHCQQTCQKYNLQFISHSLDKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQD
EEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
DQAETFLIQAIRGSGLAGLAGIPHYKELHHGGVLRPLLKYSKIEIEGFAKLNNISYIYDD
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHEEEEEEEEECCEEEEEEC
SNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCH
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQI
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCCCCCEEEEECCEEEE
HIQYNQLIIKYPTTINDISKEDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPN
EEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHCCCCCCCH
KLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG
HHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA