Click here to switch to the map view.

The map label for this gene is thsB [H]

Identifier: 134300337

GI number: 134300337

Start: 2717980

End: 2719548

Strand: Reverse

Name: thsB [H]

Synonym: Dred_2498

Alternate gene names: 134300337

Gene position: 2719548-2717980 (Counterclockwise)

Preceding gene: 134300338

Following gene: 134300336

Centisome position: 75.37

GC content: 46.85

Gene sequence:

>1569_bases
GTGAGTCTTAAACAACAGGCAAGTCAAGGTGCCGAAGTGAATGAAAAATTAGCTGCTCTGATGACTAATTCCAATGCTGT
ACGGGCCATTGCCTCGGCAGTGGAGGGAACACTGGGACCAAAGGGTCTGGACACCATGCTGGTGGATAAATTTGGAGAAG
TTGTCATTACTAACGATGGTGTAACCATATTAACCATGATGGAAGCAAACCATCCTGCTGCCCGTATGGTTATTAACATA
GCAAAATCTCAGCAGGATGAAATTGGTGATGGTACCACCACAGCCACTGTTATGGCAGGGGCTTTGGTCAGTTCCGGCGT
AGACCAAGTGGCTAAAGGCGTTCCAGTGGCCCGTGTTATTGAAGGGGTTCGAACCGGGGTACGGAGAGCATTGGAGGTTA
TGAAGCAACAGGCCATACCTCTGGAAGATATAAGTCACCCTTGGCTTAAACAAGTGGCTCTGGTGGCAGGGCGGGAGCAT
CAGGATATTGCCGATTTGGTTGTGGAAGCTGCCCGCTTAATTGGCAAAGAAAAATTGGTGGAGCGAAACTTTAAATTATC
CGATACCATTATTTCCGAAGAAGGGGCCAGTAATCAAGTTTTCATGGGTGTTATTATTAATAAAGAAACCATGAATAAAC
AAATGCCCCGGCAATTAAGAAATGTTAAGGTTCTGGTTATAGACGATGCCTTGGAACCGGAGGTCATTGGAGATGAAGCC
CTAAGTACAGAATCAGGGTTTGCCCGTTATTTGGAATTGCAATCCGAATTTCGAAGCAATGTACAAAAAATGATTGATCT
TGGAGTGGGCTTAGTTTTGGTAGATCGTGGAGTAGACGATACTGCCGAGGAAATACTCACTGATGCTGGAATTATGGTGG
TGCAAAGGGTTTTAAATAAGGAATTGTGTCGGGCGGCAGAACATACCGGGGCCCGCGTGATCAAAAGAACTAGCTTGAAA
AAGAGTATTGCCGATCTAGAAAAATACTTAGGTAATGCCGGGGAAGTTCTAATGGATGAGAGGTTGGAGCAGGTTTGGAT
TTTAGAAGGAACAGGCAAACCAATGGCCACAGTGTTAGTGGGGGCTGCCACAGAGGAAGTGGTTGGGGAACGAGAGCGAA
TTGCTAAGGATGCAGCCAGTTCGGTGCAGGCTGCTATTAAGGGTGGCGTGGTGCCCGGAGGAGGATCTTTGGAGTTAGCC
GTTTCCCGTGAAGTTGAAAAGGCCCGGGAAAATACCCGGGGTATGGTAGCCTATGGCATAAGCTGTGTAGCAGAAACCCT
TCGTCGACCGATGGCACAGATTGTTGCCAATGCAGGCTTTAACCCTCTGGAAAAACTGGGTGATGTGCTGGCAGCCCAGG
CGGAACAAGAAAAAATCTCCCTGGGTATAGACTGTGACACAGGTGATATTGTGGATATGGTTGAAATGGGAGTTATGGAC
CCAGCTTTGGTAAAAATCCATGCATTAAAAGCAGCCGGAGAAATTGCCGAAGCCATCCTGCGAATTGATACCATTATCAA
AATGAAGGATTATAAACCTGCTGGAGAAGGTTTTGGGCAGGAAGCTTAA

Upstream 100 bases:

>100_bases
GGGTGGTAACCGTGATCGATTGTATGTCTCGCAATCTTTCTCGAACACTGGACCGAATTCTGAAGGGGCAGGTTTAGTTG
AGTAAGGAGGAACTGTTTCC

Downstream 100 bases:

>100_bases
TAGAGAGGTGATACAGTGACCGAAGAGAATGCAAAAAGCGGTCAAAAAGTTGTAGAAGAGAGTGTGCAACAGACCGATGA
AAATTGTGAGTCTGTTAAGA

Product: chaperonin Cpn60/TCP-1

Products: NA

Alternate protein names: Chaperonin subunit beta; Thermosome subunit 2 [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSLKQQASQGAEVNEKLAALMTNSNAVRAIASAVEGTLGPKGLDTMLVDKFGEVVITNDGVTILTMMEANHPAARMVINI
AKSQQDEIGDGTTTATVMAGALVSSGVDQVAKGVPVARVIEGVRTGVRRALEVMKQQAIPLEDISHPWLKQVALVAGREH
QDIADLVVEAARLIGKEKLVERNFKLSDTIISEEGASNQVFMGVIINKETMNKQMPRQLRNVKVLVIDDALEPEVIGDEA
LSTESGFARYLELQSEFRSNVQKMIDLGVGLVLVDRGVDDTAEEILTDAGIMVVQRVLNKELCRAAEHTGARVIKRTSLK
KSIADLEKYLGNAGEVLMDERLEQVWILEGTGKPMATVLVGAATEEVVGERERIAKDAASSVQAAIKGGVVPGGGSLELA
VSREVEKARENTRGMVAYGISCVAETLRRPMAQIVANAGFNPLEKLGDVLAAQAEQEKISLGIDCDTGDIVDMVEMGVMD
PALVKIHALKAAGEIAEAILRIDTIIKMKDYKPAGEGFGQEA

Sequences:

>Translated_522_residues
MSLKQQASQGAEVNEKLAALMTNSNAVRAIASAVEGTLGPKGLDTMLVDKFGEVVITNDGVTILTMMEANHPAARMVINI
AKSQQDEIGDGTTTATVMAGALVSSGVDQVAKGVPVARVIEGVRTGVRRALEVMKQQAIPLEDISHPWLKQVALVAGREH
QDIADLVVEAARLIGKEKLVERNFKLSDTIISEEGASNQVFMGVIINKETMNKQMPRQLRNVKVLVIDDALEPEVIGDEA
LSTESGFARYLELQSEFRSNVQKMIDLGVGLVLVDRGVDDTAEEILTDAGIMVVQRVLNKELCRAAEHTGARVIKRTSLK
KSIADLEKYLGNAGEVLMDERLEQVWILEGTGKPMATVLVGAATEEVVGERERIAKDAASSVQAAIKGGVVPGGGSLELA
VSREVEKARENTRGMVAYGISCVAETLRRPMAQIVANAGFNPLEKLGDVLAAQAEQEKISLGIDCDTGDIVDMVEMGVMD
PALVKIHALKAAGEIAEAILRIDTIIKMKDYKPAGEGFGQEA
>Mature_521_residues
SLKQQASQGAEVNEKLAALMTNSNAVRAIASAVEGTLGPKGLDTMLVDKFGEVVITNDGVTILTMMEANHPAARMVINIA
KSQQDEIGDGTTTATVMAGALVSSGVDQVAKGVPVARVIEGVRTGVRRALEVMKQQAIPLEDISHPWLKQVALVAGREHQ
DIADLVVEAARLIGKEKLVERNFKLSDTIISEEGASNQVFMGVIINKETMNKQMPRQLRNVKVLVIDDALEPEVIGDEAL
STESGFARYLELQSEFRSNVQKMIDLGVGLVLVDRGVDDTAEEILTDAGIMVVQRVLNKELCRAAEHTGARVIKRTSLKK
SIADLEKYLGNAGEVLMDERLEQVWILEGTGKPMATVLVGAATEEVVGERERIAKDAASSVQAAIKGGVVPGGGSLELAV
SREVEKARENTRGMVAYGISCVAETLRRPMAQIVANAGFNPLEKLGDVLAAQAEQEKISLGIDCDTGDIVDMVEMGVMDP
ALVKIHALKAAGEIAEAILRIDTIIKMKDYKPAGEGFGQEA

Specific function: Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TCP-1 chaperonin family [H]

Homologues:

Organism=Homo sapiens, GI63162572, Length=504, Percent_Identity=27.1825396825397, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI24307939, Length=513, Percent_Identity=27.4853801169591, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI57863257, Length=520, Percent_Identity=29.0384615384615, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI5453607, Length=522, Percent_Identity=27.9693486590038, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI58761484, Length=460, Percent_Identity=25.4347826086956, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI38455427, Length=520, Percent_Identity=25.3846153846154, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI261399877, Length=488, Percent_Identity=27.2540983606557, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI58331173, Length=524, Percent_Identity=25.1908396946565, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4502643, Length=530, Percent_Identity=24.9056603773585, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI5453603, Length=544, Percent_Identity=25.9191176470588, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI48762932, Length=517, Percent_Identity=24.5647969052224, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI302058290, Length=513, Percent_Identity=24.7563352826511, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI57863259, Length=328, Percent_Identity=27.7439024390244, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI261399875, Length=443, Percent_Identity=25.2821670428894, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI302058292, Length=524, Percent_Identity=23.0916030534351, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI58331171, Length=531, Percent_Identity=22.9755178907721, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI58331185, Length=300, Percent_Identity=27.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI41399285, Length=159, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI31542947, Length=159, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790586, Length=518, Percent_Identity=26.0617760617761, Blast_Score=112, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI25144674, Length=508, Percent_Identity=28.9370078740157, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25148561, Length=523, Percent_Identity=27.151051625239, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17532601, Length=533, Percent_Identity=26.6416510318949, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17532603, Length=492, Percent_Identity=25.8130081300813, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17564182, Length=531, Percent_Identity=26.9303201506591, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI25147750, Length=507, Percent_Identity=26.232741617357, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25144678, Length=543, Percent_Identity=24.8618784530387, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32566944, Length=443, Percent_Identity=25.9593679458239, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555558, Length=541, Percent_Identity=24.0295748613678, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71998178, Length=547, Percent_Identity=24.4972577696526, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71981457, Length=332, Percent_Identity=27.710843373494, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI25144680, Length=441, Percent_Identity=24.9433106575964, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193210679, Length=191, Percent_Identity=28.7958115183246, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322446, Length=520, Percent_Identity=28.2692307692308, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322524, Length=513, Percent_Identity=26.7056530214425, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6320418, Length=543, Percent_Identity=28.1767955801105, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6322350, Length=546, Percent_Identity=26.9230769230769, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322049, Length=514, Percent_Identity=27.431906614786, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320393, Length=552, Percent_Identity=25.3623188405797, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320058, Length=510, Percent_Identity=24.5098039215686, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322452, Length=526, Percent_Identity=23.7642585551331, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323288, Length=158, Percent_Identity=29.1139240506329, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17647245, Length=508, Percent_Identity=27.755905511811, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24652903, Length=506, Percent_Identity=27.4703557312253, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24649027, Length=534, Percent_Identity=28.0898876404494, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24649029, Length=534, Percent_Identity=28.0898876404494, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24647512, Length=537, Percent_Identity=27.56052141527, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24647510, Length=537, Percent_Identity=27.56052141527, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24645179, Length=523, Percent_Identity=26.9598470363289, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24583944, Length=515, Percent_Identity=26.0194174757282, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18858175, Length=512, Percent_Identity=27.734375, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI28571140, Length=512, Percent_Identity=27.734375, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19921848, Length=516, Percent_Identity=23.8372093023256, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI18859933, Length=531, Percent_Identity=24.6704331450094, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI45550936, Length=538, Percent_Identity=25.6505576208178, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI45550132, Length=538, Percent_Identity=25.6505576208178, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI45550935, Length=538, Percent_Identity=25.6505576208178, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24641193, Length=163, Percent_Identity=33.7423312883436, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24641191, Length=163, Percent_Identity=33.7423312883436, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24584129, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19921262, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017998
- InterPro:   IPR002194
- InterPro:   IPR002423
- InterPro:   IPR012714 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 55878; Mature: 55747

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKQQASQGAEVNEKLAALMTNSNAVRAIASAVEGTLGPKGLDTMLVDKFGEVVITNDG
CCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCC
VTILTMMEANHPAARMVINIAKSQQDEIGDGTTTATVMAGALVSSGVDQVAKGVPVARVI
EEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHH
EGVRTGVRRALEVMKQQAIPLEDISHPWLKQVALVAGREHQDIADLVVEAARLIGKEKLV
HHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ERNFKLSDTIISEEGASNQVFMGVIINKETMNKQMPRQLRNVKVLVIDDALEPEVIGDEA
HCCCCCHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHH
LSTESGFARYLELQSEFRSNVQKMIDLGVGLVLVDRGVDDTAEEILTDAGIMVVQRVLNK
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ELCRAAEHTGARVIKRTSLKKSIADLEKYLGNAGEVLMDERLEQVWILEGTGKPMATVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCCCHHHHHH
GAATEEVVGERERIAKDAASSVQAAIKGGVVPGGGSLELAVSREVEKARENTRGMVAYGI
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHH
SCVAETLRRPMAQIVANAGFNPLEKLGDVLAAQAEQEKISLGIDCDTGDIVDMVEMGVMD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHCCCC
PALVKIHALKAAGEIAEAILRIDTIIKMKDYKPAGEGFGQEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLKQQASQGAEVNEKLAALMTNSNAVRAIASAVEGTLGPKGLDTMLVDKFGEVVITNDG
CCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCC
VTILTMMEANHPAARMVINIAKSQQDEIGDGTTTATVMAGALVSSGVDQVAKGVPVARVI
EEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHH
EGVRTGVRRALEVMKQQAIPLEDISHPWLKQVALVAGREHQDIADLVVEAARLIGKEKLV
HHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ERNFKLSDTIISEEGASNQVFMGVIINKETMNKQMPRQLRNVKVLVIDDALEPEVIGDEA
HCCCCCHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHH
LSTESGFARYLELQSEFRSNVQKMIDLGVGLVLVDRGVDDTAEEILTDAGIMVVQRVLNK
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ELCRAAEHTGARVIKRTSLKKSIADLEKYLGNAGEVLMDERLEQVWILEGTGKPMATVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCCCHHHHHH
GAATEEVVGERERIAKDAASSVQAAIKGGVVPGGGSLELAVSREVEKARENTRGMVAYGI
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHH
SCVAETLRRPMAQIVANAGFNPLEKLGDVLAAQAEQEKISLGIDCDTGDIVDMVEMGVMD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHCCCC
PALVKIHALKAAGEIAEAILRIDTIIKMKDYKPAGEGFGQEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]