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The map label for this gene is grpE [H]

Identifier: 134300336

GI number: 134300336

Start: 2717386

End: 2717964

Strand: Reverse

Name: grpE [H]

Synonym: Dred_2497

Alternate gene names: 134300336

Gene position: 2717964-2717386 (Counterclockwise)

Preceding gene: 134300337

Following gene: 134300335

Centisome position: 75.33

GC content: 46.98

Gene sequence:

>579_bases
GTGACCGAAGAGAATGCAAAAAGCGGTCAAAAAGTTGTAGAAGAGAGTGTGCAACAGACCGATGAAAATTGTGAGTCTGT
TAAGATTCCGGAGGAGGAAGCAGTCAGTCTGCCAGATGATCCGGAGGAACTTAAAAAAATGTTACAGGTAAAAACAGAGG
AATCAGAACAAAACTACAATCGAGCACTGAGGTTGCAAGCAGATTATGAAAATCTGCGCCGCCGTACCCGCCAGGAGCGG
GAGGACTTGATCAAGTTTGGCTCTGAGCAACTGATCCAAGGTTTATTACCAGTTATGGACAACTTTGAAAGGGCCTTGGC
CAATGCTGGTGACGGAGGGGAAAAATTTATTAGCGGAGTGGAAATGATTTATCGCCAGCTTAATGAAGTCCTTTCCAGAG
AGGGCTTGGAACCCATTCCGGCCCAAGGGGAGCAGTTTGACCCCAATGTTCATGATGCAGTGATGCAAGTGCAGGATTCC
GACGAACCGGAAAACACGGTGGTGGAGGAACTCCGCAAGGGCTACTATCTAAAAGGAAAAGTAATCAGACCATCCATGGT
TAAAGTCGCTAGCTCCTAA

Upstream 100 bases:

>100_bases
TGCCGAAGCCATCCTGCGAATTGATACCATTATCAAAATGAAGGATTATAAACCTGCTGGAGAAGGTTTTGGGCAGGAAG
CTTAATAGAGAGGTGATACA

Downstream 100 bases:

>100_bases
TATTTAGAACTTAAGATCATGGATTGTAATAAATTTTCTCAGGAGGTTAAGAAAATGGGAAAAGTAATAGGAATTGATTT
AGGAACAACCAACTCTTGTG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MTEENAKSGQKVVEESVQQTDENCESVKIPEEEAVSLPDDPEELKKMLQVKTEESEQNYNRALRLQADYENLRRRTRQER
EDLIKFGSEQLIQGLLPVMDNFERALANAGDGGEKFISGVEMIYRQLNEVLSREGLEPIPAQGEQFDPNVHDAVMQVQDS
DEPENTVVEELRKGYYLKGKVIRPSMVKVASS

Sequences:

>Translated_192_residues
MTEENAKSGQKVVEESVQQTDENCESVKIPEEEAVSLPDDPEELKKMLQVKTEESEQNYNRALRLQADYENLRRRTRQER
EDLIKFGSEQLIQGLLPVMDNFERALANAGDGGEKFISGVEMIYRQLNEVLSREGLEPIPAQGEQFDPNVHDAVMQVQDS
DEPENTVVEELRKGYYLKGKVIRPSMVKVASS
>Mature_191_residues
TEENAKSGQKVVEESVQQTDENCESVKIPEEEAVSLPDDPEELKKMLQVKTEESEQNYNRALRLQADYENLRRRTRQERE
DLIKFGSEQLIQGLLPVMDNFERALANAGDGGEKFISGVEMIYRQLNEVLSREGLEPIPAQGEQFDPNVHDAVMQVQDSD
EPENTVVEELRKGYYLKGKVIRPSMVKVASS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI40255109, Length=183, Percent_Identity=29.5081967213115, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1788967, Length=133, Percent_Identity=37.593984962406, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17552458, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=163, Percent_Identity=30.6748466257669, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24653432, Length=199, Percent_Identity=30.1507537688442, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21859; Mature: 21727

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEENAKSGQKVVEESVQQTDENCESVKIPEEEAVSLPDDPEELKKMLQVKTEESEQNYN
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RALRLQADYENLRRRTRQEREDLIKFGSEQLIQGLLPVMDNFERALANAGDGGEKFISGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EMIYRQLNEVLSREGLEPIPAQGEQFDPNVHDAVMQVQDSDEPENTVVEELRKGYYLKGK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCC
VIRPSMVKVASS
CCCHHHHHHCCC
>Mature Secondary Structure 
TEENAKSGQKVVEESVQQTDENCESVKIPEEEAVSLPDDPEELKKMLQVKTEESEQNYN
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RALRLQADYENLRRRTRQEREDLIKFGSEQLIQGLLPVMDNFERALANAGDGGEKFISGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EMIYRQLNEVLSREGLEPIPAQGEQFDPNVHDAVMQVQDSDEPENTVVEELRKGYYLKGK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCC
VIRPSMVKVASS
CCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]