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The map label for this gene is dgkA [H]

Identifier: 134300320

GI number: 134300320

Start: 2702546

End: 2702914

Strand: Reverse

Name: dgkA [H]

Synonym: Dred_2481

Alternate gene names: 134300320

Gene position: 2702914-2702546 (Counterclockwise)

Preceding gene: 134300321

Following gene: 134300319

Centisome position: 74.91

GC content: 42.82

Gene sequence:

>369_bases
ATGATGAAGGATTTTGTTAAAAGTTTTACTTTTGCTTTAGCAGGAATTTGGTATGTGTTTCGAACCCAAAGAAATATGCA
GGTTCACTTTACTGCGGCACTGCTGGCAGTGACGGTGAGCCTTTTTTTGCGTTTGTCTCCTTTGGAATGGGGGTTGGTCT
TTTTTGCTATTTTTATGGTAATATTGACAGAGTGTATCAATACCGCCATTGAAGCCGTTGTGAACTTAGTTTCACCGGAG
TTCCACCCCCTGGCCAAAATAGCTAAAGACTGCGCCGCCGGAGCAGTCTTACTGGCGGCTATCAGTTCGCTCGTTGTGGC
ATATTTGATCTTATGGCCCAAGATTATCATACTATTCTTTAGTCATTAG

Upstream 100 bases:

>100_bases
TAGATAGCCCACAACAGGGTTAGCCATCAGCTATCAGCCCTCAGCCATCGGCTTACGGTGTAAGGCCAAAAGTTGTTAAA
CCTACTTTGAAAGGTTACCT

Downstream 100 bases:

>100_bases
CTATTAGCCATGGCTTAAGGGATAGTCTTTTGGGTCTAGCCATTAATCCTTAGCAATTTGCTAAACCCAAAAGCTAAAGA
CTAAAGGCTTACCCCAAAGA

Product: diacylglycerol kinase

Products: NA

Alternate protein names: UdpK [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MMKDFVKSFTFALAGIWYVFRTQRNMQVHFTAALLAVTVSLFLRLSPLEWGLVFFAIFMVILTECINTAIEAVVNLVSPE
FHPLAKIAKDCAAGAVLLAAISSLVVAYLILWPKIIILFFSH

Sequences:

>Translated_122_residues
MMKDFVKSFTFALAGIWYVFRTQRNMQVHFTAALLAVTVSLFLRLSPLEWGLVFFAIFMVILTECINTAIEAVVNLVSPE
FHPLAKIAKDCAAGAVLLAAISSLVVAYLILWPKIIILFFSH
>Mature_122_residues
MMKDFVKSFTFALAGIWYVFRTQRNMQVHFTAALLAVTVSLFLRLSPLEWGLVFFAIFMVILTECINTAIEAVVNLVSPE
FHPLAKIAKDCAAGAVLLAAISSLVVAYLILWPKIIILFFSH

Specific function: Catalyzes the phosphorylation of undecaprenol, which is probably the primary physiological substrate. Is also able to phosphorylate diacylglycerol, albeit with very low efficiency. May play a role in adaptability to environmental stress conditions such as

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000829 [H]

Pfam domain/function: PF01219 DAGK_prokar [H]

EC number: =2.7.1.66 [H]

Molecular weight: Translated: 13637; Mature: 13637

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKDFVKSFTFALAGIWYVFRTQRNMQVHFTAALLAVTVSLFLRLSPLEWGLVFFAIFMV
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILTECINTAIEAVVNLVSPEFHPLAKIAKDCAAGAVLLAAISSLVVAYLILWPKIIILFF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SH
CC
>Mature Secondary Structure
MMKDFVKSFTFALAGIWYVFRTQRNMQVHFTAALLAVTVSLFLRLSPLEWGLVFFAIFMV
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILTECINTAIEAVVNLVSPEFHPLAKIAKDCAAGAVLLAAISSLVVAYLILWPKIIILFF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8407794; 9422608; 12397186 [H]