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The map label for this gene is hisA

Identifier: 134300190

GI number: 134300190

Start: 2572003

End: 2572743

Strand: Reverse

Name: hisA

Synonym: Dred_2351

Alternate gene names: 134300190

Gene position: 2572743-2572003 (Counterclockwise)

Preceding gene: 134300191

Following gene: 134300189

Centisome position: 71.3

GC content: 51.55

Gene sequence:

>741_bases
ATGATCCTGTTTCCGGCCATAGATTTAAAAGAGGGTCAATGTGTACGTCTTGTGGAAGGGCGTATGGACAGTGCCACAGT
ATACTCCAATGATCCTGGCAGCATGGCCCGGTTGTGGCAGGATCAGGGTGCCCAGTACATCCATGTGGTGGATTTGGATG
GTGCCTTTGCCGGTCAACCACGTAACCGCCAATCCATTGCCCAAATTGTGCAGGGGGTTCAGGTGCCGGTCCAGGTGGGC
GGAGGTATCCGTGATTTGGAAACCATTGAAGACTTATTGTCCCTGGGAGTTGACCGTGTTATTCTAGGCAGTGCAGCCAT
TCTAAAACCAGAATTGGTGGCTGAGGCCTGCCGTAAATATGGTCAGCGGATTCTGCTGGGAATTGATGCCAAGGACGGTC
AGGTGGCAATTCAGGGTTGGGGAGAAACTGTTAAAAGAACAGCCTTGGACTTAGCCCTGGAAATGAAACAGTTGGGAATC
GAACGGGCAGTTTTTACGGATATTAGGCGTGACGGTAAACTATCTGGACCAAATCTGGCAGCCACTGGCGAATTGGCCAG
GAACAGTGGACTTAGGGTAATCGCCTCCGGCGGTGTTGCTTCTCTGGAAGATATTCGGCAATTGAAAAAATTGGAACAAG
ATGGGGTGGAGGGAGCCATCCTGGGAAAGGCTCTTTATACCAATGCTGTCAAGTTGCCTGAGGCCCTGGTCATTGCCCGG
GGGGAGGAATCAGTATGCTGA

Upstream 100 bases:

>100_bases
TCGTGGGCAAGGCCAATGTATTTGGTATTCAGTTTCATCCCGAAAAGAGCAGCACTCTGGGAATTCGTATCTTAGAAAAC
TTTGGAAAGATGGTGAGGGC

Downstream 100 bases:

>100_bases
TGAAACGAGTGATTCCCTGCTTGGATGTAACTGCTGGCAGAGTGGTTAAGGGGACAAATTTTGTCAACCTGAGAGATGCC
GGAGACCCAGTGGAACTGGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MILFPAIDLKEGQCVRLVEGRMDSATVYSNDPGSMARLWQDQGAQYIHVVDLDGAFAGQPRNRQSIAQIVQGVQVPVQVG
GGIRDLETIEDLLSLGVDRVILGSAAILKPELVAEACRKYGQRILLGIDAKDGQVAIQGWGETVKRTALDLALEMKQLGI
ERAVFTDIRRDGKLSGPNLAATGELARNSGLRVIASGGVASLEDIRQLKKLEQDGVEGAILGKALYTNAVKLPEALVIAR
GEESVC

Sequences:

>Translated_246_residues
MILFPAIDLKEGQCVRLVEGRMDSATVYSNDPGSMARLWQDQGAQYIHVVDLDGAFAGQPRNRQSIAQIVQGVQVPVQVG
GGIRDLETIEDLLSLGVDRVILGSAAILKPELVAEACRKYGQRILLGIDAKDGQVAIQGWGETVKRTALDLALEMKQLGI
ERAVFTDIRRDGKLSGPNLAATGELARNSGLRVIASGGVASLEDIRQLKKLEQDGVEGAILGKALYTNAVKLPEALVIAR
GEESVC
>Mature_246_residues
MILFPAIDLKEGQCVRLVEGRMDSATVYSNDPGSMARLWQDQGAQYIHVVDLDGAFAGQPRNRQSIAQIVQGVQVPVQVG
GGIRDLETIEDLLSLGVDRVILGSAAILKPELVAEACRKYGQRILLGIDAKDGQVAIQGWGETVKRTALDLALEMKQLGI
ERAVFTDIRRDGKLSGPNLAATGELARNSGLRVIASGGVASLEDIRQLKKLEQDGVEGAILGKALYTNAVKLPEALVIAR
GEESVC

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=34.7107438016529, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=23.8683127572016, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_DESRM (A4J708)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113686.1
- ProteinModelPortal:   A4J708
- SMR:   A4J708
- STRING:   A4J708
- GeneID:   4956374
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2351
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   A4J708
- ProtClustDB:   PRK00748
- BioCyc:   DRED349161:DRED_2351-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26340; Mature: 26340

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFPAIDLKEGQCVRLVEGRMDSATVYSNDPGSMARLWQDQGAQYIHVVDLDGAFAGQP
CEEECCCCCCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
RNRQSIAQIVQGVQVPVQVGGGIRDLETIEDLLSLGVDRVILGSAAILKPELVAEACRKY
CCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCHHHCCHHHHHHHHHHH
GQRILLGIDAKDGQVAIQGWGETVKRTALDLALEMKQLGIERAVFTDIRRDGKLSGPNLA
CCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCE
ATGELARNSGLRVIASGGVASLEDIRQLKKLEQDGVEGAILGKALYTNAVKLPEALVIAR
ECCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEC
GEESVC
CCCCCC
>Mature Secondary Structure
MILFPAIDLKEGQCVRLVEGRMDSATVYSNDPGSMARLWQDQGAQYIHVVDLDGAFAGQP
CEEECCCCCCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
RNRQSIAQIVQGVQVPVQVGGGIRDLETIEDLLSLGVDRVILGSAAILKPELVAEACRKY
CCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCHHHCCHHHHHHHHHHH
GQRILLGIDAKDGQVAIQGWGETVKRTALDLALEMKQLGIERAVFTDIRRDGKLSGPNLA
CCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCE
ATGELARNSGLRVIASGGVASLEDIRQLKKLEQDGVEGAILGKALYTNAVKLPEALVIAR
ECCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEC
GEESVC
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA