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The map label for this gene is hisH [H]

Identifier: 134300191

GI number: 134300191

Start: 2572740

End: 2573351

Strand: Reverse

Name: hisH [H]

Synonym: Dred_2352

Alternate gene names: 134300191

Gene position: 2573351-2572740 (Counterclockwise)

Preceding gene: 134300192

Following gene: 134300190

Centisome position: 71.32

GC content: 47.06

Gene sequence:

>612_bases
ATGATTGCCATTATTGATTATGGCATGGGCAATTTACGCAGTGTGCAAAAGGGCTTTGCCAGGGCAGGCTACCAGGCAGA
AATAGTCCAACAACCAGAGCAGGTAGACGATGCACGGGCGGTGGTTTTGCCCGGTGTAGGTGCCTTTGCCGATGCCATGG
AAAACCTTAAAAAGAGCGGCATGGTTGAAGCCATCAAAAGGGTTGTTCAGAGGGAGAAACCCTTTTTAGGCATTTGTTTA
GGACAACAGCTATTATTTGAATCCAGTGAAGAGTTTGGTAAAACCAAAGGGCTGGGCATTCTGCCTGGTACAGTAAAACG
GTTTCCCGCCGGTACCCTAAAGGTACCCCACATGGGCTGGAATCAAATAGAAATCAAACAAGCAAGCCCTTTACTAGAGG
GAATCTCAAATGGTTCAGCTTTCTATTTTGTTCATTCCTACCATGTGGCGCCAACCGATGATAACCTGATCCTATGCCTG
ACGGACTATGGGGTAAGGTTTCCTGCCGTCGTGGGCAAGGCCAATGTATTTGGTATTCAGTTTCATCCCGAAAAGAGCAG
CACTCTGGGAATTCGTATCTTAGAAAACTTTGGAAAGATGGTGAGGGCATGA

Upstream 100 bases:

>100_bases
ATTGAGGGAGCCTTTAAGGCCCTAGGAAGGGCTTTACGACAGGCGGTGTCCAAGGATCAACGGTTAACAGGTATTCCTTC
CACTAAGGGAGTGTTGTAAG

Downstream 100 bases:

>100_bases
TCCTGTTTCCGGCCATAGATTTAAAAGAGGGTCAATGTGTACGTCTTGTGGAAGGGCGTATGGACAGTGCCACAGTATAC
TCCAATGATCCTGGCAGCAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIAIIDYGMGNLRSVQKGFARAGYQAEIVQQPEQVDDARAVVLPGVGAFADAMENLKKSGMVEAIKRVVQREKPFLGICL
GQQLLFESSEEFGKTKGLGILPGTVKRFPAGTLKVPHMGWNQIEIKQASPLLEGISNGSAFYFVHSYHVAPTDDNLILCL
TDYGVRFPAVVGKANVFGIQFHPEKSSTLGIRILENFGKMVRA

Sequences:

>Translated_203_residues
MIAIIDYGMGNLRSVQKGFARAGYQAEIVQQPEQVDDARAVVLPGVGAFADAMENLKKSGMVEAIKRVVQREKPFLGICL
GQQLLFESSEEFGKTKGLGILPGTVKRFPAGTLKVPHMGWNQIEIKQASPLLEGISNGSAFYFVHSYHVAPTDDNLILCL
TDYGVRFPAVVGKANVFGIQFHPEKSSTLGIRILENFGKMVRA
>Mature_203_residues
MIAIIDYGMGNLRSVQKGFARAGYQAEIVQQPEQVDDARAVVLPGVGAFADAMENLKKSGMVEAIKRVVQREKPFLGICL
GQQLLFESSEEFGKTKGLGILPGTVKRFPAGTLKVPHMGWNQIEIKQASPLLEGISNGSAFYFVHSYHVAPTDDNLILCL
TDYGVRFPAVVGKANVFGIQFHPEKSSTLGIRILENFGKMVRA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=39.6984924623116, Blast_Score=146, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=32.3809523809524, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22168; Mature: 22168

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGMGNLRSVQKGFARAGYQAEIVQQPEQVDDARAVVLPGVGAFADAMENLKKSG
CEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCC
MVEAIKRVVQREKPFLGICLGQQLLFESSEEFGKTKGLGILPGTVKRFPAGTLKVPHMGW
HHHHHHHHHHHCCCEEEEEHHHHHHHCCHHHHCCCCCCCCCCCHHHHCCCCCEECCCCCC
NQIEIKQASPLLEGISNGSAFYFVHSYHVAPTDDNLILCLTDYGVRFPAVVGKANVFGIQ
CEEEEEHHCHHHHHCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCCCCEECCCEEEEEE
FHPEKSSTLGIRILENFGKMVRA
ECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAIIDYGMGNLRSVQKGFARAGYQAEIVQQPEQVDDARAVVLPGVGAFADAMENLKKSG
CEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCC
MVEAIKRVVQREKPFLGICLGQQLLFESSEEFGKTKGLGILPGTVKRFPAGTLKVPHMGW
HHHHHHHHHHHCCCEEEEEHHHHHHHCCHHHHCCCCCCCCCCCHHHHCCCCCEECCCCCC
NQIEIKQASPLLEGISNGSAFYFVHSYHVAPTDDNLILCLTDYGVRFPAVVGKANVFGIQ
CEEEEEHHCHHHHHCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCCCCEECCCEEEEEE
FHPEKSSTLGIRILENFGKMVRA
ECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA