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The map label for this gene is hisF

Identifier: 134300189

GI number: 134300189

Start: 2571251

End: 2572009

Strand: Reverse

Name: hisF

Synonym: Dred_2350

Alternate gene names: 134300189

Gene position: 2572009-2571251 (Counterclockwise)

Preceding gene: 134300190

Following gene: 134300188

Centisome position: 71.28

GC content: 51.38

Gene sequence:

>759_bases
ATGCTGATGAAACGAGTGATTCCCTGCTTGGATGTAACTGCTGGCAGAGTGGTTAAGGGGACAAATTTTGTCAACCTGAG
AGATGCCGGAGACCCAGTGGAACTGGCTGCCTTTTATGACAAAGAAGGAGCCGATGAATTGGTGTTTCTGGATATAACAG
CTTCTTCAGATGGCCGTGTCACTATGCTGGATGTGGTGCGACGTACGGCGGAGGAGGTCTTTATCCCTTTCACTGTGGGC
GGCGGCATTCGAACGGTGGATGACATGAGACTGATGCTAAAGGCCGGGGCCGATAAAATTGGTATTAATACGGCAGCCAT
CAAGAACCCCCGGCTTATCTCCGAAGGGGCCATTAAGTTCGGCAGCCAATGTATCGTTGTGGCCATGGATGCCCGTCAGG
TTGCTTCGGAAAAATGGGAAGTATATATTCATGGTGGCCGTACTTCCACTGGTTTGGATGCGGTGGAGTGGGCCTTGAAA
GCCGAGGAATTAGGTGCCGGTGAGATTTTGCTTACCAGTATGGACAGGGACGGGACCAAAGAGGGGTTTGATTTGGCCCT
CACCAGAACAATTGCCCAACATGTGAAAATCCCCGTAATCGCCTCCGGTGGGGTGGGCAATCTGGAACACATGGCCCGGG
GCTTAACCGATGGCATGGCTGATGCAGCCCTGGCGGCATCGATTTTTCACTTTGGGGAATATACTATTCGGCAGGCCAAA
GAGTATCTAATGGAACAGGGTATTCCGGTAAGACTTTAA

Upstream 100 bases:

>100_bases
AACAAGATGGGGTGGAGGGAGCCATCCTGGGAAAGGCTCTTTATACCAATGCTGTCAAGTTGCCTGAGGCCCTGGTCATT
GCCCGGGGGGAGGAATCAGT

Downstream 100 bases:

>100_bases
AAAGGCTTGCATGGAAAATGAAGGAAGCTGGTGAGCGGAAATGCAGTTTGACAAAGAGGTTTTAAAGTATAATGAAGCGG
GACTGATTCCGGCCATTGTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLMKRVIPCLDVTAGRVVKGTNFVNLRDAGDPVELAAFYDKEGADELVFLDITASSDGRVTMLDVVRRTAEEVFIPFTVG
GGIRTVDDMRLMLKAGADKIGINTAAIKNPRLISEGAIKFGSQCIVVAMDARQVASEKWEVYIHGGRTSTGLDAVEWALK
AEELGAGEILLTSMDRDGTKEGFDLALTRTIAQHVKIPVIASGGVGNLEHMARGLTDGMADAALAASIFHFGEYTIRQAK
EYLMEQGIPVRL

Sequences:

>Translated_252_residues
MLMKRVIPCLDVTAGRVVKGTNFVNLRDAGDPVELAAFYDKEGADELVFLDITASSDGRVTMLDVVRRTAEEVFIPFTVG
GGIRTVDDMRLMLKAGADKIGINTAAIKNPRLISEGAIKFGSQCIVVAMDARQVASEKWEVYIHGGRTSTGLDAVEWALK
AEELGAGEILLTSMDRDGTKEGFDLALTRTIAQHVKIPVIASGGVGNLEHMARGLTDGMADAALAASIFHFGEYTIRQAK
EYLMEQGIPVRL
>Mature_252_residues
MLMKRVIPCLDVTAGRVVKGTNFVNLRDAGDPVELAAFYDKEGADELVFLDITASSDGRVTMLDVVRRTAEEVFIPFTVG
GGIRTVDDMRLMLKAGADKIGINTAAIKNPRLISEGAIKFGSQCIVVAMDARQVASEKWEVYIHGGRTSTGLDAVEWALK
AEELGAGEILLTSMDRDGTKEGFDLALTRTIAQHVKIPVIASGGVGNLEHMARGLTDGMADAALAASIFHFGEYTIRQAK
EYLMEQGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.9143968871595, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=25.2100840336134, Blast_Score=91, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.6687898089172, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_DESRM (A4J707)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113685.1
- ProteinModelPortal:   A4J707
- SMR:   A4J707
- STRING:   A4J707
- GeneID:   4956414
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2350
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A4J707
- ProtClustDB:   PRK02083
- BioCyc:   DRED349161:DRED_2350-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27186; Mature: 27186

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMKRVIPCLDVTAGRVVKGTNFVNLRDAGDPVELAAFYDKEGADELVFLDITASSDGRV
CCHHHHCCHHCCCCCCEEECCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCC
TMLDVVRRTAEEVFIPFTVGGGIRTVDDMRLMLKAGADKIGINTAAIKNPRLISEGAIKF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCEECCCHHHC
GSQCIVVAMDARQVASEKWEVYIHGGRTSTGLDAVEWALKAEELGAGEILLTSMDRDGTK
CCEEEEEEECHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
EGFDLALTRTIAQHVKIPVIASGGVGNLEHMARGLTDGMADAALAASIFHFGEYTIRQAK
CCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EYLMEQGIPVRL
HHHHHCCCCCCC
>Mature Secondary Structure
MLMKRVIPCLDVTAGRVVKGTNFVNLRDAGDPVELAAFYDKEGADELVFLDITASSDGRV
CCHHHHCCHHCCCCCCEEECCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCC
TMLDVVRRTAEEVFIPFTVGGGIRTVDDMRLMLKAGADKIGINTAAIKNPRLISEGAIKF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCEECCCHHHC
GSQCIVVAMDARQVASEKWEVYIHGGRTSTGLDAVEWALKAEELGAGEILLTSMDRDGTK
CCEEEEEEECHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
EGFDLALTRTIAQHVKIPVIASGGVGNLEHMARGLTDGMADAALAASIFHFGEYTIRQAK
CCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EYLMEQGIPVRL
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA