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The map label for this gene is hisI [H]

Identifier: 134300188

GI number: 134300188

Start: 2570530

End: 2571210

Strand: Reverse

Name: hisI [H]

Synonym: Dred_2349

Alternate gene names: 134300188

Gene position: 2571210-2570530 (Counterclockwise)

Preceding gene: 134300189

Following gene: 134300187

Centisome position: 71.26

GC content: 47.43

Gene sequence:

>681_bases
ATGCAGTTTGACAAAGAGGTTTTAAAGTATAATGAAGCGGGACTGATTCCGGCCATTGTGCAGGATGTACATACAAAGGA
AGTATTAATGATGGCCTGGATGAACCAAGAAGCAGTTGAAAAAACCCAGGCTAGCGGAGAAACCTGGTTTTACAGCCGCA
GCCGCCAAAGAATGTGGAAAAAGGGTGAAACCTCCGGCCATGTACAAAGGGTAAAGGGGCTTTATTATGACTGTGATGCC
GATACACTTTTGGTGTTGGTGGAACAGGTGGGTGGAGCCGCTTGCCACGAGGGTTATACATCCTGCTTCCATAATAAAAT
AAACCAGGATAACACTGTCATTGTGGAGGGCGAGAAGAAATTTGACCCTGCAGCGGTTTATGGCAATACAACAGAAACAG
TTGGGGCAGAAATTATCCAGCAACTGTTTCAAGTTATCCTGAGCCGTAAAGAGGACCGCCCGGAAGGTTCCTATACCACC
TATCTATTTGAAAAGGGCGTAGATAAAATCTGCAAGAAGATTGGGGAAGAAGCCGCCGAGGTTATCATTGGAGCCAAGAA
CAGCCAAGAAGAACTCACCTACGAGGCAGCGGATCTGATCTATCACCTGCTGGTGTTGCTGGCCAACCAAGGGGTTAGTA
CCCAGGAGGTGTACCGGGAACTGGCAAAGCGTCGGAAATAA

Upstream 100 bases:

>100_bases
TATACTATTCGGCAGGCCAAAGAGTATCTAATGGAACAGGGTATTCCGGTAAGACTTTAAAAAGGCTTGCATGGAAAATG
AAGGAAGCTGGTGAGCGGAA

Downstream 100 bases:

>100_bases
GGTGTCTGGTGAATTTCTGTGAGAAGATCGGAGTAAGCAGTGTTAGCGTGAATACTAAAAAAGGTTTATATTAAAGCTCA
GCACCACTGTAGATAGTGGT

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MQFDKEVLKYNEAGLIPAIVQDVHTKEVLMMAWMNQEAVEKTQASGETWFYSRSRQRMWKKGETSGHVQRVKGLYYDCDA
DTLLVLVEQVGGAACHEGYTSCFHNKINQDNTVIVEGEKKFDPAAVYGNTTETVGAEIIQQLFQVILSRKEDRPEGSYTT
YLFEKGVDKICKKIGEEAAEVIIGAKNSQEELTYEAADLIYHLLVLLANQGVSTQEVYRELAKRRK

Sequences:

>Translated_226_residues
MQFDKEVLKYNEAGLIPAIVQDVHTKEVLMMAWMNQEAVEKTQASGETWFYSRSRQRMWKKGETSGHVQRVKGLYYDCDA
DTLLVLVEQVGGAACHEGYTSCFHNKINQDNTVIVEGEKKFDPAAVYGNTTETVGAEIIQQLFQVILSRKEDRPEGSYTT
YLFEKGVDKICKKIGEEAAEVIIGAKNSQEELTYEAADLIYHLLVLLANQGVSTQEVYRELAKRRK
>Mature_226_residues
MQFDKEVLKYNEAGLIPAIVQDVHTKEVLMMAWMNQEAVEKTQASGETWFYSRSRQRMWKKGETSGHVQRVKGLYYDCDA
DTLLVLVEQVGGAACHEGYTSCFHNKINQDNTVIVEGEKKFDPAAVYGNTTETVGAEIIQQLFQVILSRKEDRPEGSYTT
YLFEKGVDKICKKIGEEAAEVIIGAKNSQEELTYEAADLIYHLLVLLANQGVSTQEVYRELAKRRK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=214, Percent_Identity=40.6542056074766, Blast_Score=156, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319819, Length=208, Percent_Identity=33.1730769230769, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH [H]

EC number: =3.5.4.19; =3.6.1.31 [H]

Molecular weight: Translated: 25625; Mature: 25625

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDKEVLKYNEAGLIPAIVQDVHTKEVLMMAWMNQEAVEKTQASGETWFYSRSRQRMWK
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEHHHHHHHHHH
KGETSGHVQRVKGLYYDCDADTLLVLVEQVGGAACHEGYTSCFHNKINQDNTVIVEGEKK
CCCCCHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
FDPAAVYGNTTETVGAEIIQQLFQVILSRKEDRPEGSYTTYLFEKGVDKICKKIGEEAAE
CCCHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VIIGAKNSQEELTYEAADLIYHLLVLLANQGVSTQEVYRELAKRRK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQFDKEVLKYNEAGLIPAIVQDVHTKEVLMMAWMNQEAVEKTQASGETWFYSRSRQRMWK
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEHHHHHHHHHH
KGETSGHVQRVKGLYYDCDADTLLVLVEQVGGAACHEGYTSCFHNKINQDNTVIVEGEKK
CCCCCHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
FDPAAVYGNTTETVGAEIIQQLFQVILSRKEDRPEGSYTTYLFEKGVDKICKKIGEEAAE
CCCHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VIIGAKNSQEELTYEAADLIYHLLVLLANQGVSTQEVYRELAKRRK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]