Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 134300187

GI number: 134300187

Start: 2567926

End: 2570118

Strand: Reverse

Name: pcrA [H]

Synonym: Dred_2348

Alternate gene names: 134300187

Gene position: 2570118-2567926 (Counterclockwise)

Preceding gene: 134300188

Following gene: 134300186

Centisome position: 71.23

GC content: 45.96

Gene sequence:

>2193_bases
ATGAATATTTTATCGCAATTGAATCCTGCCCAAGAAGAGGCGGTACAACATACGGAAGGCCCCCTGTTGGTACTGGCAGG
TGCTGGCTCCGGTAAGACCAGAGTGCTGACACATCGAATTGCCCAAATTCTCAGCCAAGGAGTTCGGCCCTATAACATTT
TAGCCATCACCTTTACCAATAAGGCTGCTAATGAAATGCGAGCAAGGGTAGAAAATTTAGTACCCCAAGCAGCCAAGGAC
CTTTGGGTAACTACCTTTCACAGTGCCTGTCTGCGTATCCTCAGAAGAGAAATCGATGTTATGGGCTATGGTTCTAACTT
TTCCATTTACGACGATGCTGATCAACAAACTGTCATCAAAGAATGCCTAAAGGAATTAGAAATTGATGAAAAAAGGTTTC
AACCCCGGGCTATGCTGTCAGCGATCTCAGGAGCAAAGAACAAGCTTCAGACACCGGCCCAATATGATCGTCAGGCCTTT
GAATATTTTGAACAGGTAGCTGCCAGGGTCTATCGCCTGTACCAGGAAAAACTATTTAAAAATAATGCGGTGGATTTTGA
TGACCTAATCATGTTAACAGTGCGTTTGTTTAAGGAAAACCCAGATGTCCTAGCCTATTACCAAAATAAATTTAAATATA
TTTTGGTGGATGAGTACCAGGACACCAACCATACTCAGTATGTTTTGGTGAACATGCTGGCAGAAAGTCATCGCAATGTT
TGTGTGGTAGGTGATCCCAACCAGTCTATTTATAAATGGCGGGGAGCCGATATTAACAACATTCTGGACTTTGAACGGGA
CTATCCCGAGGCAAAAGTGGTCAAACTGGAACAAAACTACCGTTCCACCGGTAACATTCTACAGGCTGCCAATGCTGTTA
TTAAAAACAATGCTGAGGCTAAGGATATGAAACTCTGGACCTCCAAAGGGGAGGGTAATCGAATTCATATCTACCGGGCA
GAGAACGAACGCTTTGAGTCTATGTACATTGCGGACCGTGTTAAAGATTTGCGTTTCTCCCATAACCGCAAGTATCGTGA
TATGGTCATACTCTACCGGACCCATGCCCAATCCCGTGTTTTTGAAGAAGTTTTTCTTCGTCAAGGCATTCCCTATAGTA
TCTTTGGTGGGCAGAAGTTCTATGACCGTAAAGAAATTAAAGATCTTCTTTCCTATTTAAGAGTAATTGCGAACCCTGTA
GACAGCCAGAGCCTGTTGCGTATAATTAACGTACCCAAAAGGGGAGTGGGGGCTGCATCCATTGAAAAGCTGACCAACTT
TGCCACAGAAAGAGATCTCACTGTCTTAATGGCTCTGGCTCTGGTGGATGATATTCCTGGTGTGCCGGCTAAAGCCCGCA
AGCAATGTAGAGAACTGGCAGACAACCTTGCCAGTTTGAAGAAACAGGTGGAGTTCCTTTCTGTAACGGAGATCACCGAA
GAGGTTCTAAATCGCACTGGCTATAAGGCTGAACTGGAACTGGAAAATACCGTTGAGGCCAGAACCCGCTTGGAGAACTT
GCAGGAATTCCTCACGGTTACAAAAGAATATGATAAACAACAGGGGGAAGAAACCGGGGGGCTGGCGGATTTCCTTTCTA
CCATTTCCCTGGTAACCGATTTGGATCGGCACGACCCCCAAGCAGATCAAATTACTTTAATGACACTACACAGTGCCAAA
GGGCTGGAATATCCGGTAGTCTTTTTAACCGGCATGGAAGAAGGCGTGTTCCCCCACAGCCGGGCCCTTTACGACGAAGA
GGAACTGGAGGAGGAACGTCGACTGGCCTATGTGGGTATTACCCGGGCTGAAGAAGAACTATACATTACCCACTGTTGGG
AAAGAACCCTGTTTGGGCGTACCAACATGAATGCCAAATCCCGCTTCTTAGAGGAAATACCCCTAGAACTGACCGTTGGC
CAATCCCCTGTTAAAAAGCTAGCTGCGGGATCCGGGCTTATAAAATCCAGTTTATTTAATAAAACCGGTACAATAGCGAC
CCAAGCCCCAGCCGCCCCTGCGGCAGTTCCTAAGAGTTATTTACTGGGAGACAAAGTTCGGCATGCCAAATGGGGTGACG
GGGTTATCGTGAAAGTAAAGGGCAATGGCGGTAATGCAGAAATTACTGTGGCATTTCCTGAAATGGGCCTAAAAACCCTG
ATTGCCCAGTATGCACCACTGGAAAAGATCTAA

Upstream 100 bases:

>100_bases
CACAATGTTTGATATAATGTACTGTGTTTTTCCTAACATGTGGAAAGAGAAGGTCTTTCCAACCTTAGTAACCCTAGAAA
CTACCAGGTGGAGGAAATAG

Downstream 100 bases:

>100_bases
ATTGGTAACAATAAGGGCGGAGGAAAAATATGGACCGCTTAATGGCTGTGGTGGCTTTAACTGCCATCATCCACTTAATT
AATACCTTAATATACGCAGT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MNILSQLNPAQEEAVQHTEGPLLVLAGAGSGKTRVLTHRIAQILSQGVRPYNILAITFTNKAANEMRARVENLVPQAAKD
LWVTTFHSACLRILRREIDVMGYGSNFSIYDDADQQTVIKECLKELEIDEKRFQPRAMLSAISGAKNKLQTPAQYDRQAF
EYFEQVAARVYRLYQEKLFKNNAVDFDDLIMLTVRLFKENPDVLAYYQNKFKYILVDEYQDTNHTQYVLVNMLAESHRNV
CVVGDPNQSIYKWRGADINNILDFERDYPEAKVVKLEQNYRSTGNILQAANAVIKNNAEAKDMKLWTSKGEGNRIHIYRA
ENERFESMYIADRVKDLRFSHNRKYRDMVILYRTHAQSRVFEEVFLRQGIPYSIFGGQKFYDRKEIKDLLSYLRVIANPV
DSQSLLRIINVPKRGVGAASIEKLTNFATERDLTVLMALALVDDIPGVPAKARKQCRELADNLASLKKQVEFLSVTEITE
EVLNRTGYKAELELENTVEARTRLENLQEFLTVTKEYDKQQGEETGGLADFLSTISLVTDLDRHDPQADQITLMTLHSAK
GLEYPVVFLTGMEEGVFPHSRALYDEEELEEERRLAYVGITRAEEELYITHCWERTLFGRTNMNAKSRFLEEIPLELTVG
QSPVKKLAAGSGLIKSSLFNKTGTIATQAPAAPAAVPKSYLLGDKVRHAKWGDGVIVKVKGNGGNAEITVAFPEMGLKTL
IAQYAPLEKI

Sequences:

>Translated_730_residues
MNILSQLNPAQEEAVQHTEGPLLVLAGAGSGKTRVLTHRIAQILSQGVRPYNILAITFTNKAANEMRARVENLVPQAAKD
LWVTTFHSACLRILRREIDVMGYGSNFSIYDDADQQTVIKECLKELEIDEKRFQPRAMLSAISGAKNKLQTPAQYDRQAF
EYFEQVAARVYRLYQEKLFKNNAVDFDDLIMLTVRLFKENPDVLAYYQNKFKYILVDEYQDTNHTQYVLVNMLAESHRNV
CVVGDPNQSIYKWRGADINNILDFERDYPEAKVVKLEQNYRSTGNILQAANAVIKNNAEAKDMKLWTSKGEGNRIHIYRA
ENERFESMYIADRVKDLRFSHNRKYRDMVILYRTHAQSRVFEEVFLRQGIPYSIFGGQKFYDRKEIKDLLSYLRVIANPV
DSQSLLRIINVPKRGVGAASIEKLTNFATERDLTVLMALALVDDIPGVPAKARKQCRELADNLASLKKQVEFLSVTEITE
EVLNRTGYKAELELENTVEARTRLENLQEFLTVTKEYDKQQGEETGGLADFLSTISLVTDLDRHDPQADQITLMTLHSAK
GLEYPVVFLTGMEEGVFPHSRALYDEEELEEERRLAYVGITRAEEELYITHCWERTLFGRTNMNAKSRFLEEIPLELTVG
QSPVKKLAAGSGLIKSSLFNKTGTIATQAPAAPAAVPKSYLLGDKVRHAKWGDGVIVKVKGNGGNAEITVAFPEMGLKTL
IAQYAPLEKI
>Mature_730_residues
MNILSQLNPAQEEAVQHTEGPLLVLAGAGSGKTRVLTHRIAQILSQGVRPYNILAITFTNKAANEMRARVENLVPQAAKD
LWVTTFHSACLRILRREIDVMGYGSNFSIYDDADQQTVIKECLKELEIDEKRFQPRAMLSAISGAKNKLQTPAQYDRQAF
EYFEQVAARVYRLYQEKLFKNNAVDFDDLIMLTVRLFKENPDVLAYYQNKFKYILVDEYQDTNHTQYVLVNMLAESHRNV
CVVGDPNQSIYKWRGADINNILDFERDYPEAKVVKLEQNYRSTGNILQAANAVIKNNAEAKDMKLWTSKGEGNRIHIYRA
ENERFESMYIADRVKDLRFSHNRKYRDMVILYRTHAQSRVFEEVFLRQGIPYSIFGGQKFYDRKEIKDLLSYLRVIANPV
DSQSLLRIINVPKRGVGAASIEKLTNFATERDLTVLMALALVDDIPGVPAKARKQCRELADNLASLKKQVEFLSVTEITE
EVLNRTGYKAELELENTVEARTRLENLQEFLTVTKEYDKQQGEETGGLADFLSTISLVTDLDRHDPQADQITLMTLHSAK
GLEYPVVFLTGMEEGVFPHSRALYDEEELEEERRLAYVGITRAEEELYITHCWERTLFGRTNMNAKSRFLEEIPLELTVG
QSPVKKLAAGSGLIKSSLFNKTGTIATQAPAAPAAVPKSYLLGDKVRHAKWGDGVIVKVKGNGGNAEITVAFPEMGLKTL
IAQYAPLEKI

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=741, Percent_Identity=39.8110661268556, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI48994965, Length=654, Percent_Identity=40.0611620795107, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1787196, Length=332, Percent_Identity=28.0120481927711, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=750, Percent_Identity=28.2666666666667, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324477, Length=688, Percent_Identity=25.5813953488372, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82891; Mature: 82891

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILSQLNPAQEEAVQHTEGPLLVLAGAGSGKTRVLTHRIAQILSQGVRPYNILAITFTN
CCCHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECC
KAANEMRARVENLVPQAAKDLWVTTFHSACLRILRREIDVMGYGSNFSIYDDADQQTVIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCHHHHHH
ECLKELEIDEKRFQPRAMLSAISGAKNKLQTPAQYDRQAFEYFEQVAARVYRLYQEKLFK
HHHHHCCCCHHHCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNAVDFDDLIMLTVRLFKENPDVLAYYQNKFKYILVDEYQDTNHTQYVLVNMLAESHRNV
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCE
CVVGDPNQSIYKWRGADINNILDFERDYPEAKVVKLEQNYRSTGNILQAANAVIKNNAEA
EEEECCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHCCCCH
KDMKLWTSKGEGNRIHIYRAENERFESMYIADRVKDLRFSHNRKYRDMVILYRTHAQSRV
HHEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHH
FEEVFLRQGIPYSIFGGQKFYDRKEIKDLLSYLRVIANPVDSQSLLRIINVPKRGVGAAS
HHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
IEKLTNFATERDLTVLMALALVDDIPGVPAKARKQCRELADNLASLKKQVEFLSVTEITE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLNRTGYKAELELENTVEARTRLENLQEFLTVTKEYDKQQGEETGGLADFLSTISLVTD
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LDRHDPQADQITLMTLHSAKGLEYPVVFLTGMEEGVFPHSRALYDEEELEEERRLAYVGI
HHCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHCEEEEEE
TRAEEELYITHCWERTLFGRTNMNAKSRFLEEIPLELTVGQSPVKKLAAGSGLIKSSLFN
EECCCCEEEEHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCCCHHHHHHHC
KTGTIATQAPAAPAAVPKSYLLGDKVRHAKWGDGVIVKVKGNGGNAEITVAFPEMGLKTL
CCCCCEECCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHH
IAQYAPLEKI
HHHHCCCCCC
>Mature Secondary Structure
MNILSQLNPAQEEAVQHTEGPLLVLAGAGSGKTRVLTHRIAQILSQGVRPYNILAITFTN
CCCHHHCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECC
KAANEMRARVENLVPQAAKDLWVTTFHSACLRILRREIDVMGYGSNFSIYDDADQQTVIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCHHHHHH
ECLKELEIDEKRFQPRAMLSAISGAKNKLQTPAQYDRQAFEYFEQVAARVYRLYQEKLFK
HHHHHCCCCHHHCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNAVDFDDLIMLTVRLFKENPDVLAYYQNKFKYILVDEYQDTNHTQYVLVNMLAESHRNV
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCE
CVVGDPNQSIYKWRGADINNILDFERDYPEAKVVKLEQNYRSTGNILQAANAVIKNNAEA
EEEECCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHCCCCH
KDMKLWTSKGEGNRIHIYRAENERFESMYIADRVKDLRFSHNRKYRDMVILYRTHAQSRV
HHEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHH
FEEVFLRQGIPYSIFGGQKFYDRKEIKDLLSYLRVIANPVDSQSLLRIINVPKRGVGAAS
HHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
IEKLTNFATERDLTVLMALALVDDIPGVPAKARKQCRELADNLASLKKQVEFLSVTEITE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLNRTGYKAELELENTVEARTRLENLQEFLTVTKEYDKQQGEETGGLADFLSTISLVTD
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LDRHDPQADQITLMTLHSAKGLEYPVVFLTGMEEGVFPHSRALYDEEELEEERRLAYVGI
HHCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHCEEEEEE
TRAEEELYITHCWERTLFGRTNMNAKSRFLEEIPLELTVGQSPVKKLAAGSGLIKSSLFN
EECCCCEEEEHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCCCHHHHHHHC
KTGTIATQAPAAPAAVPKSYLLGDKVRHAKWGDGVIVKVKGNGGNAEITVAFPEMGLKTL
CCCCCEECCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHH
IAQYAPLEKI
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]