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The map label for this gene is metF [H]

Identifier: 134300036

GI number: 134300036

Start: 2397226

End: 2398092

Strand: Reverse

Name: metF [H]

Synonym: Dred_2192

Alternate gene names: 134300036

Gene position: 2398092-2397226 (Counterclockwise)

Preceding gene: 134300037

Following gene: 134300035

Centisome position: 66.46

GC content: 41.98

Gene sequence:

>867_bases
ATGAAAATTTCTACCCTTTACCAATCCCAAAATCCTGTGATCTCGTTTGAAATATTTCCTCCCAAGAGCACCTCGCCGGT
GGAAACCATCTATGAGACCTTGGAGGGATTAATTAAATTAGAACCTGGCTTTATTAGTGTTACCTACGGGGCGGGGGGAA
GCAGTCGTGATAGAACCAAGGAAATCGCTGCAACGATAAAAAACCGGTATAAAATTGAAGCCCTGGCTCATTTAACCTGT
GTTGGTCAAGCTGTAAACGAGATTGACCAAATTCTTAATGAATTACAAGAAGAAAATATTGAAAATGTACTGGCCCTAAG
GGGTGACCCGCCAGCAGATGCACAAAATTATGACTTCTCAGATTCAGATTTTAAATATGCCTCTGATCTGGTTAGGCACA
TCAAAAATAGTAGTAATGTCTGTGTGGCAGCAGCTGCTTATCCCGAAGGACACGCCCATTGCCGGCGCTTAAGTGACGAC
CTGAACTGGTTAAAATATAAAGTTGACCAGGGGGTGGATTTCCTTATTAGCCAGCTTTTCTTTGATAATAGGATATTTTA
TAACTTCCTTGAAAATGCCCTAAGAGTTAATATTCATTGCCCGGTGGTTGCGGGAATCTTACCCGTACTTAATGCAAAAC
AAATTAAGCGAATTATTTCCCTATGTGGAGCTTCTATGCCGGCAAAACTCTTGATGCTGGTTGACAAATATGGCGATAAT
GCAGAAGACATGGAGAAAGCCGGCATTGAGTATGCCAGCCAGCAGGTTCGTGATCTACTGGAAAGCGGAGTGGATGGTAT
TCACCTTTATACCATGAATAAACCACAGCAGGTTACTGAGATACTAAAGAATGTAGGAAAGGCATAA

Upstream 100 bases:

>100_bases
GAACGATGTAAGGTTTAGGAAACAAATAGTTATTTCCCCATTGAAAGAGTCGCGTTATAATAGACAAAAGACAATCCCAA
AAGGACAAAGGCGGTGCAAT

Downstream 100 bases:

>100_bases
ATACCGCGCTGCGCTGTGGCGCGGTATTTTATATTCTTCTCTATGATCATCTCAAGGAGGGACCCTTGTTTGAAATCTAC
TAAAATTGCTTTGGTTCAGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKISTLYQSQNPVISFEIFPPKSTSPVETIYETLEGLIKLEPGFISVTYGAGGSSRDRTKEIAATIKNRYKIEALAHLTC
VGQAVNEIDQILNELQEENIENVLALRGDPPADAQNYDFSDSDFKYASDLVRHIKNSSNVCVAAAAYPEGHAHCRRLSDD
LNWLKYKVDQGVDFLISQLFFDNRIFYNFLENALRVNIHCPVVAGILPVLNAKQIKRIISLCGASMPAKLLMLVDKYGDN
AEDMEKAGIEYASQQVRDLLESGVDGIHLYTMNKPQQVTEILKNVGKA

Sequences:

>Translated_288_residues
MKISTLYQSQNPVISFEIFPPKSTSPVETIYETLEGLIKLEPGFISVTYGAGGSSRDRTKEIAATIKNRYKIEALAHLTC
VGQAVNEIDQILNELQEENIENVLALRGDPPADAQNYDFSDSDFKYASDLVRHIKNSSNVCVAAAAYPEGHAHCRRLSDD
LNWLKYKVDQGVDFLISQLFFDNRIFYNFLENALRVNIHCPVVAGILPVLNAKQIKRIISLCGASMPAKLLMLVDKYGDN
AEDMEKAGIEYASQQVRDLLESGVDGIHLYTMNKPQQVTEILKNVGKA
>Mature_288_residues
MKISTLYQSQNPVISFEIFPPKSTSPVETIYETLEGLIKLEPGFISVTYGAGGSSRDRTKEIAATIKNRYKIEALAHLTC
VGQAVNEIDQILNELQEENIENVLALRGDPPADAQNYDFSDSDFKYASDLVRHIKNSSNVCVAAAAYPEGHAHCRRLSDD
LNWLKYKVDQGVDFLISQLFFDNRIFYNFLENALRVNIHCPVVAGILPVLNAKQIKRIISLCGASMPAKLLMLVDKYGDN
AEDMEKAGIEYASQQVRDLLESGVDGIHLYTMNKPQQVTEILKNVGKA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=294, Percent_Identity=32.312925170068, Blast_Score=168, Evalue=7e-42,
Organism=Escherichia coli, GI1790377, Length=273, Percent_Identity=34.7985347985348, Blast_Score=177, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI25147441, Length=293, Percent_Identity=29.3515358361775, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI25147436, Length=293, Percent_Identity=29.3515358361775, Blast_Score=140, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6325234, Length=300, Percent_Identity=34.6666666666667, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6321313, Length=272, Percent_Identity=31.25, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24662658, Length=229, Percent_Identity=23.1441048034935, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32102; Mature: 32102

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISTLYQSQNPVISFEIFPPKSTSPVETIYETLEGLIKLEPGFISVTYGAGGSSRDRTK
CCCCHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHHH
EIAATIKNRYKIEALAHLTCVGQAVNEIDQILNELQEENIENVLALRGDPPADAQNYDFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCC
DSDFKYASDLVRHIKNSSNVCVAAAAYPEGHAHCRRLSDDLNWLKYKVDQGVDFLISQLF
CCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FDNRIFYNFLENALRVNIHCPVVAGILPVLNAKQIKRIISLCGASMPAKLLMLVDKYGDN
HCCHHHHHHHHHHHEEEEECHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
AEDMEKAGIEYASQQVRDLLESGVDGIHLYTMNKPQQVTEILKNVGKA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKISTLYQSQNPVISFEIFPPKSTSPVETIYETLEGLIKLEPGFISVTYGAGGSSRDRTK
CCCCHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHHH
EIAATIKNRYKIEALAHLTCVGQAVNEIDQILNELQEENIENVLALRGDPPADAQNYDFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCC
DSDFKYASDLVRHIKNSSNVCVAAAAYPEGHAHCRRLSDDLNWLKYKVDQGVDFLISQLF
CCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FDNRIFYNFLENALRVNIHCPVVAGILPVLNAKQIKRIISLCGASMPAKLLMLVDKYGDN
HCCHHHHHHHHHHHEEEEECHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
AEDMEKAGIEYASQQVRDLLESGVDGIHLYTMNKPQQVTEILKNVGKA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]