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The map label for this gene is mtnU [H]

Identifier: 134300035

GI number: 134300035

Start: 2396323

End: 2397156

Strand: Reverse

Name: mtnU [H]

Synonym: Dred_2191

Alternate gene names: 134300035

Gene position: 2397156-2396323 (Counterclockwise)

Preceding gene: 134300036

Following gene: 134300034

Centisome position: 66.44

GC content: 40.53

Gene sequence:

>834_bases
TTGAAATCTACTAAAATTGCTTTGGTTCAGATGCAAGCAACATTTGGTAATATTGACAAAAATCTAAGTACACTTGAAAA
GTTTATAAACGAAGCAGCAGCACAACAGGCAGAGATTATTTGTTTTCCTGAAATGTGTATCCAAGGCTATAGCAGAGAAA
TCCCGGACTTCTTACTCCAGAGTATAGACGGAGAAGCGATCTTGTTTCTTAAAAAATTGGCACAGAATAAAGGAATTACT
ATTATTGCCGGTATGGCAGAAAAATGTCTTAATAAGAGGCCCTTCATTACCCAGGTAGTGATCAGGCCAGGCCAAAATAT
TGATTATTACCGAAAGACTCATTTAGGAAATAGTGAACAGCCGTACTATCAGGCCGGCAATGAAATCAAAACCTTTTCTA
CAGAAAAAACAACCATTGGCATTCAAATATGTTGGGATACCCACTTCCCGGAAATGACCACAATTCTTTCGCTACGGGGA
GCCGAGGTTATTTTTGCACCCCATGCTTCCCCAACTATTGTAGGAGACCGTAAAGCCATCTGGTTAAAATATCTGGCAGC
CAGAGCCTATGATAACTCTGTTTTCCTGGCCGCCTGCAATTTAGTGGGGGATGACGGAAATGGTCGTCAATTTTGTGGTG
GCTCAATGGTTATCGACCCTAAGGGAAATGTGTTGGCAGAAGACTTTGAAGGAAAGCAATCGATGCTAGTGGTGGATTTG
GATTGTGCTCAGATAAATGCCATACGCGAGAGAAAATCATCTTCGATGGCTAATAGTTTCTTTCTCGCTAACAGAAGACC
GGAAATATATAAAGACCTATTACGTATTTTTTAG

Upstream 100 bases:

>100_bases
TGAGATACTAAAGAATGTAGGAAAGGCATAAATACCGCGCTGCGCTGTGGCGCGGTATTTTATATTCTTCTCTATGATCA
TCTCAAGGAGGGACCCTTGT

Downstream 100 bases:

>100_bases
TATATCAGACCAACATGCCGCTTGGCGACACCACTGATAAATGAAAAGTTTTAATTTTCAGGGCAGAAAGAAAAAATTTC
AGATATACTATGGTAACTAA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKSTKIALVQMQATFGNIDKNLSTLEKFINEAAAQQAEIICFPEMCIQGYSREIPDFLLQSIDGEAILFLKKLAQNKGIT
IIAGMAEKCLNKRPFITQVVIRPGQNIDYYRKTHLGNSEQPYYQAGNEIKTFSTEKTTIGIQICWDTHFPEMTTILSLRG
AEVIFAPHASPTIVGDRKAIWLKYLAARAYDNSVFLAACNLVGDDGNGRQFCGGSMVIDPKGNVLAEDFEGKQSMLVVDL
DCAQINAIRERKSSSMANSFFLANRRPEIYKDLLRIF

Sequences:

>Translated_277_residues
MKSTKIALVQMQATFGNIDKNLSTLEKFINEAAAQQAEIICFPEMCIQGYSREIPDFLLQSIDGEAILFLKKLAQNKGIT
IIAGMAEKCLNKRPFITQVVIRPGQNIDYYRKTHLGNSEQPYYQAGNEIKTFSTEKTTIGIQICWDTHFPEMTTILSLRG
AEVIFAPHASPTIVGDRKAIWLKYLAARAYDNSVFLAACNLVGDDGNGRQFCGGSMVIDPKGNVLAEDFEGKQSMLVVDL
DCAQINAIRERKSSSMANSFFLANRRPEIYKDLLRIF
>Mature_277_residues
MKSTKIALVQMQATFGNIDKNLSTLEKFINEAAAQQAEIICFPEMCIQGYSREIPDFLLQSIDGEAILFLKKLAQNKGIT
IIAGMAEKCLNKRPFITQVVIRPGQNIDYYRKTHLGNSEQPYYQAGNEIKTFSTEKTTIGIQICWDTHFPEMTTILSLRG
AEVIFAPHASPTIVGDRKAIWLKYLAARAYDNSVFLAACNLVGDDGNGRQFCGGSMVIDPKGNVLAEDFEGKQSMLVVDL
DCAQINAIRERKSSSMANSFFLANRRPEIYKDLLRIF

Specific function: May be involved in a regulatory step in the methylthioribose (MTR) recycling pathway [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=262, Percent_Identity=29.0076335877863, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI297632350, Length=286, Percent_Identity=26.2237762237762, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI297632348, Length=286, Percent_Identity=26.2237762237762, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI5031947, Length=286, Percent_Identity=26.2237762237762, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI7706509, Length=256, Percent_Identity=26.953125, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786412, Length=253, Percent_Identity=26.0869565217391, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556280, Length=281, Percent_Identity=27.4021352313167, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17533173, Length=269, Percent_Identity=29.368029739777, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323383, Length=287, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17933642, Length=258, Percent_Identity=28.2945736434109, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355835, Length=270, Percent_Identity=25.5555555555556, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21358471, Length=253, Percent_Identity=27.6679841897233, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30980; Mature: 30980

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTKIALVQMQATFGNIDKNLSTLEKFINEAAAQQAEIICFPEMCIQGYSREIPDFLLQ
CCCCEEEEEEEECHHCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHHHH
SIDGEAILFLKKLAQNKGITIIAGMAEKCLNKRPFITQVVIRPGQNIDYYRKTHLGNSEQ
HCCCCHHHHHHHHHCCCCEEEEECCHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCCCCC
PYYQAGNEIKTFSTEKTTIGIQICWDTHFPEMTTILSLRGAEVIFAPHASPTIVGDRKAI
CHHHCCCCEEEEECCCEEEEEEEEECCCCCHHHHHHEECCCEEEEECCCCCEEECCCHHH
WLKYLAARAYDNSVFLAACNLVGDDGNGRQFCGGSMVIDPKGNVLAEDFEGKQSMLVVDL
HHHHHHHHHCCCEEEEEEEEEECCCCCCCEEECCCEEECCCCCEEECCCCCCCEEEEEEE
DCAQINAIRERKSSSMANSFFLANRRPEIYKDLLRIF
CHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHC
>Mature Secondary Structure
MKSTKIALVQMQATFGNIDKNLSTLEKFINEAAAQQAEIICFPEMCIQGYSREIPDFLLQ
CCCCEEEEEEEECHHCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHHHH
SIDGEAILFLKKLAQNKGITIIAGMAEKCLNKRPFITQVVIRPGQNIDYYRKTHLGNSEQ
HCCCCHHHHHHHHHCCCCEEEEECCHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCCCCC
PYYQAGNEIKTFSTEKTTIGIQICWDTHFPEMTTILSLRGAEVIFAPHASPTIVGDRKAI
CHHHCCCCEEEEECCCEEEEEEEEECCCCCHHHHHHEECCCEEEEECCCCCEEECCCHHH
WLKYLAARAYDNSVFLAACNLVGDDGNGRQFCGGSMVIDPKGNVLAEDFEGKQSMLVVDL
HHHHHHHHHCCCEEEEEEEEEECCCCCCCEEECCCEEECCCCCEEECCCCCCCEEEEEEE
DCAQINAIRERKSSSMANSFFLANRRPEIYKDLLRIF
CHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]