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The map label for this gene is glcA [H]

Identifier: 134300034

GI number: 134300034

Start: 2394024

End: 2395754

Strand: Reverse

Name: glcA [H]

Synonym: Dred_2190

Alternate gene names: 134300034

Gene position: 2395754-2394024 (Counterclockwise)

Preceding gene: 134300035

Following gene: 134300033

Centisome position: 66.4

GC content: 42.0

Gene sequence:

>1731_bases
ATGTGGCTACAAGAAACGAACCCTTTTGGGAACATTGGGATTTCCGCACTGGTTGCTTCTATACCAATTCTTATTCTGTT
CTACGCCCTTGCAATTAAACGCATGAAGGGGCATATAGCAGGCATAATCACCCTTGTTGCAGCAATTTTAGTTGCTATCG
TTGCCTACGGTATGCCAGCTAAATTAGCCGTACTTTCAACGATATACGGTATTTTAACGGGGATATTTCCCATTGGTTGG
ATTGTTTTGTGTGCTGTGTTTTTATACAATCTAACTGTAAAAACGGGCCATTTTGAGGTGATAAAGGACTCCATTGCTTC
TATTACAGAAGATCGTCGATTACAAGCTTTGTTAATTGGCTATTGCTTTGGAGCCTTTTTAGAAGGTGCTGCTGGCTTTG
GTGCTCCGGTTGCCATCACTGCGGGTATGCTAGTTGGCCTAGGCTTTCAACCCCTGTATGCTGCAGGATTATGCTTGATT
GCAAACACTGCTCCGGTGGCCTTCGGTGGAATTGGCATTCCCATTATTACAGCTGGTAAAGTTTCAGGATTAGATCCCTT
TGTTGTAAGTCAAATGATAGCAAATCAATTACCTTTACTATCCTTTGTTGTTCCGTTCTGGTTGGTCTTTATTATGTCTG
GCTGGAAGGGCATGAAAGAAGTTTTTGCACCAGTTTTCGTCACTGCAGCTTCCTTCAGTGTTACCATGTGGTTCGTAGCA
TTTAAAATTGGTCCTGAATTACCCAATATTATTTCAGCACTGGTAGCCATTGTAGTACTGGTTTCTTTCTTAAAGGTCTG
GAAGCCAAAGCAAATCTGGCGTTTTCCTAATGAACCTGCAGCCAGTATGGAAGTAAAGCATCACCCCTTTGGTAGAATTG
TACAAGCTTGGACTCCCTTTATTGTGCTGACTGTATTAATTGGTGACTGGGGAATTAGTGGTATTAAGTACCTGTTGGAT
CAGTTTACTGTTAAAATTCCCATTGCTGGCTTGCATAATGCTATTATGATTGGGGACAAACCTCTAGAAGTTATCTATAA
ATTTAACTGGTTAGGGGCAGCTGGTACTGCCATTCTCATTGCAACCATTATATCAGCCATTATCCTAAGGGTACGTCCTA
AAACATATCTTTCTGTATTTGGAGAAACCTTAAACAGCTTGAAGTATTCTCTAACAACGGTTGCTTGTGTTCTAGGTTTT
GCCTATGTTGCTAACTTTGCTGGAATTACCCCTACCCTGGGACAAGCCCTCACTGTAACTGGATCTTTCTTCCCGTTTGT
TTCCCCCTTTCTGGGATGGGTAGGTGTCTTTGTTACCGGCAGTGATACCTCTGCCAATGCATTGTTCTGCAACATGCAGA
AAATAACAGCCCAACAGATTGATGTAAACCCCGTTTTAACTGTAACAGCTAACACTAGCGGTGGTGTGGCTGCCAAGATG
ATTTCACCTCAGAGTATTGCAGTGGCTTGTGCATCTGTAAATTTGGTTGGTAAAGAGAGTGATTTGTTTAGATTTACTGT
AAAACACAGTTTACTCTTTACAGCAATCATGGGTGTAATTGTATATATACAAGCATACTATTTAAAAGGGATGGTGCCTG
CAGTGGTAGAGGCTTCCGCTGTTGCGAAGGCCGCAACTCCTGATTATGGACTAAGTACAGTAGCTTTAGTAGTATCCTTT
GCTGTTATGGTGCTGCTTGGTATAGTAGCTGCTCGTCAGAGAATGGAATAA

Upstream 100 bases:

>100_bases
AATACTTACATAGAGGAGGGGGAAAAAAGGAATTTATTTTTTGTATTATCTAATAATTACGCAATTATATTTTTTTTAAG
AAATAAGGGGGATATATAAC

Downstream 100 bases:

>100_bases
AATTATAAAATAGTAACCTAAAAACCCTTTTGTGTGCAGTCACCTGACTGTATACACAAAAGGGTTTTTTGCTTTTGACA
TAGATAGTATTACCTTCCAG

Product: L-lactate transport

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MWLQETNPFGNIGISALVASIPILILFYALAIKRMKGHIAGIITLVAAILVAIVAYGMPAKLAVLSTIYGILTGIFPIGW
IVLCAVFLYNLTVKTGHFEVIKDSIASITEDRRLQALLIGYCFGAFLEGAAGFGAPVAITAGMLVGLGFQPLYAAGLCLI
ANTAPVAFGGIGIPIITAGKVSGLDPFVVSQMIANQLPLLSFVVPFWLVFIMSGWKGMKEVFAPVFVTAASFSVTMWFVA
FKIGPELPNIISALVAIVVLVSFLKVWKPKQIWRFPNEPAASMEVKHHPFGRIVQAWTPFIVLTVLIGDWGISGIKYLLD
QFTVKIPIAGLHNAIMIGDKPLEVIYKFNWLGAAGTAILIATIISAIILRVRPKTYLSVFGETLNSLKYSLTTVACVLGF
AYVANFAGITPTLGQALTVTGSFFPFVSPFLGWVGVFVTGSDTSANALFCNMQKITAQQIDVNPVLTVTANTSGGVAAKM
ISPQSIAVACASVNLVGKESDLFRFTVKHSLLFTAIMGVIVYIQAYYLKGMVPAVVEASAVAKAATPDYGLSTVALVVSF
AVMVLLGIVAARQRME

Sequences:

>Translated_576_residues
MWLQETNPFGNIGISALVASIPILILFYALAIKRMKGHIAGIITLVAAILVAIVAYGMPAKLAVLSTIYGILTGIFPIGW
IVLCAVFLYNLTVKTGHFEVIKDSIASITEDRRLQALLIGYCFGAFLEGAAGFGAPVAITAGMLVGLGFQPLYAAGLCLI
ANTAPVAFGGIGIPIITAGKVSGLDPFVVSQMIANQLPLLSFVVPFWLVFIMSGWKGMKEVFAPVFVTAASFSVTMWFVA
FKIGPELPNIISALVAIVVLVSFLKVWKPKQIWRFPNEPAASMEVKHHPFGRIVQAWTPFIVLTVLIGDWGISGIKYLLD
QFTVKIPIAGLHNAIMIGDKPLEVIYKFNWLGAAGTAILIATIISAIILRVRPKTYLSVFGETLNSLKYSLTTVACVLGF
AYVANFAGITPTLGQALTVTGSFFPFVSPFLGWVGVFVTGSDTSANALFCNMQKITAQQIDVNPVLTVTANTSGGVAAKM
ISPQSIAVACASVNLVGKESDLFRFTVKHSLLFTAIMGVIVYIQAYYLKGMVPAVVEASAVAKAATPDYGLSTVALVVSF
AVMVLLGIVAARQRME
>Mature_576_residues
MWLQETNPFGNIGISALVASIPILILFYALAIKRMKGHIAGIITLVAAILVAIVAYGMPAKLAVLSTIYGILTGIFPIGW
IVLCAVFLYNLTVKTGHFEVIKDSIASITEDRRLQALLIGYCFGAFLEGAAGFGAPVAITAGMLVGLGFQPLYAAGLCLI
ANTAPVAFGGIGIPIITAGKVSGLDPFVVSQMIANQLPLLSFVVPFWLVFIMSGWKGMKEVFAPVFVTAASFSVTMWFVA
FKIGPELPNIISALVAIVVLVSFLKVWKPKQIWRFPNEPAASMEVKHHPFGRIVQAWTPFIVLTVLIGDWGISGIKYLLD
QFTVKIPIAGLHNAIMIGDKPLEVIYKFNWLGAAGTAILIATIISAIILRVRPKTYLSVFGETLNSLKYSLTTVACVLGF
AYVANFAGITPTLGQALTVTGSFFPFVSPFLGWVGVFVTGSDTSANALFCNMQKITAQQIDVNPVLTVTANTSGGVAAKM
ISPQSIAVACASVNLVGKESDLFRFTVKHSLLFTAIMGVIVYIQAYYLKGMVPAVVEASAVAKAATPDYGLSTVALVVSF
AVMVLLGIVAARQRME

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=555, Percent_Identity=52.4324324324324, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1790031, Length=551, Percent_Identity=50.0907441016334, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 61417; Mature: 61417

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLQETNPFGNIGISALVASIPILILFYALAIKRMKGHIAGIITLVAAILVAIVAYGMPA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KLAVLSTIYGILTGIFPIGWIVLCAVFLYNLTVKTGHFEVIKDSIASITEDRRLQALLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YCFGAFLEGAAGFGAPVAITAGMLVGLGFQPLYAAGLCLIANTAPVAFGGIGIPIITAGK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCCHHEECCCCCEECCCCCCEEECCC
VSGLDPFVVSQMIANQLPLLSFVVPFWLVFIMSGWKGMKEVFAPVFVTAASFSVTMWFVA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKIGPELPNIISALVAIVVLVSFLKVWKPKQIWRFPNEPAASMEVKHHPFGRIVQAWTPF
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEECCCCHHHHHHHHHHH
IVLTVLIGDWGISGIKYLLDQFTVKIPIAGLHNAIMIGDKPLEVIYKFNWLGAAGTAILI
HHHHHHHHCCCHHHHHHHHHHHEEEEEECCCCCEEEECCCCEEEEEEEECCCCHHHHHHH
ATIISAIILRVRPKTYLSVFGETLNSLKYSLTTVACVLGFAYVANFAGITPTLGQALTVT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
GSFFPFVSPFLGWVGVFVTGSDTSANALFCNMQKITAQQIDVNPVLTVTANTSGGVAAKM
CCHHHHHHHHHHHHEEEEECCCCCCCEEEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEE
ISPQSIAVACASVNLVGKESDLFRFTVKHSLLFTAIMGVIVYIQAYYLKGMVPAVVEASA
ECCHHHHHEEHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VAKAATPDYGLSTVALVVSFAVMVLLGIVAARQRME
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWLQETNPFGNIGISALVASIPILILFYALAIKRMKGHIAGIITLVAAILVAIVAYGMPA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KLAVLSTIYGILTGIFPIGWIVLCAVFLYNLTVKTGHFEVIKDSIASITEDRRLQALLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YCFGAFLEGAAGFGAPVAITAGMLVGLGFQPLYAAGLCLIANTAPVAFGGIGIPIITAGK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCCHHEECCCCCEECCCCCCEEECCC
VSGLDPFVVSQMIANQLPLLSFVVPFWLVFIMSGWKGMKEVFAPVFVTAASFSVTMWFVA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKIGPELPNIISALVAIVVLVSFLKVWKPKQIWRFPNEPAASMEVKHHPFGRIVQAWTPF
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEECCCCHHHHHHHHHHH
IVLTVLIGDWGISGIKYLLDQFTVKIPIAGLHNAIMIGDKPLEVIYKFNWLGAAGTAILI
HHHHHHHHCCCHHHHHHHHHHHEEEEEECCCCCEEEECCCCEEEEEEEECCCCHHHHHHH
ATIISAIILRVRPKTYLSVFGETLNSLKYSLTTVACVLGFAYVANFAGITPTLGQALTVT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
GSFFPFVSPFLGWVGVFVTGSDTSANALFCNMQKITAQQIDVNPVLTVTANTSGGVAAKM
CCHHHHHHHHHHHHEEEEECCCCCCCEEEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEE
ISPQSIAVACASVNLVGKESDLFRFTVKHSLLFTAIMGVIVYIQAYYLKGMVPAVVEASA
ECCHHHHHEEHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VAKAATPDYGLSTVALVVSFAVMVLLGIVAARQRME
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]