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The map label for this gene is hemC

Identifier: 134300009

GI number: 134300009

Start: 2367344

End: 2368273

Strand: Reverse

Name: hemC

Synonym: Dred_2163

Alternate gene names: 134300009

Gene position: 2368273-2367344 (Counterclockwise)

Preceding gene: 134300010

Following gene: 134300008

Centisome position: 65.64

GC content: 46.77

Gene sequence:

>930_bases
ATGAAACATAAAATAACCGTTGGCAGCAGGGACAGTGCGCTGGCACTGTGGCAAACTCGCTGGGTAGTTGAACAGCTGGA
AAAACAAAACCCTGATGTTACCTTTGAAATTACAACCATGAAGACTAAGGGAGACAAAATGTTGGATGTGGCCCTGGCCA
AAATTGGGGATAAAGGTCTATTTACCAAAGAATTAGAAGTGGCTATGTTGCAGAAGGAAATCGATTTTGCAGTGCATAGT
CTGAAGGATATGCCCACTGCTTTGCCAGAAGGCTTAATCATTGGTGCAGTATGCAAGCGAGATAACCCCGGCGATGCACT
GATTTCCAAGGATGGACGGAAATTAGATGAGCTTCCTAAGGGTGCTAGAATCGGTACATCTTCTTTACGCCGCTGTGCGC
AGTTATTAAACTACCGTCCGGATTTTCAACTGGAGGCCCTACGGGGTAACCTGAATACACGTATGAAAAAGTTGGTGTCC
GAGCAACTAGATGGCATTATTCTGGCTGCAGCGGGCATAACCCGCATGGGCTGGGAAGATATGATTGCGGAAATTATTCC
CTTTCAGGTTTGTCTACCGGCAGTGGGACAGGGTGCCATTTCTGTGGAATGTCGGGAGGACGATCCAGAAATTCTTAATC
TACTTAAAGGTATTGAACATACAGAGACCAAAGCTGCCACTGAAGCAGAAAGGTCATTACTCCGTTACTTAGAAGGCGGT
TGCCAGGTGCCCATTGGAGCCCACAGTGAAGTTAAGAATAATAGGCTTATGCTCACCGCTGTTGTGGCCACCCTAGATGG
GACTAAAGTTATTCGGGCCCAAGGAGAGAACGAGGTTAACAAGGCGGTGGAATTAGGTATTGAAGTGGCTGAGAAACTAA
TGGCAATGGGCGGTAAAAAAATACTGGAAGAAGTTAGGGCGGGGGAATGA

Upstream 100 bases:

>100_bases
AAAAAGGTTGCCGGTGGTTCCACCCTTAACAGCGGTTGGGACAAGGGGATAGCTGCAATGGTGGCAGACAGTCGATCCAG
GGAATAAAAGGGAGGCTACC

Downstream 100 bases:

>100_bases
AAATGACAAAGGGTATTGTATATTTAGTAGGAGCTGGCCCAGGTGACCCGGGATTAATTACAGTAAAAGGATTGGAGTGT
ATTCGTAAGGCGGATGTTCT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKHKITVGSRDSALALWQTRWVVEQLEKQNPDVTFEITTMKTKGDKMLDVALAKIGDKGLFTKELEVAMLQKEIDFAVHS
LKDMPTALPEGLIIGAVCKRDNPGDALISKDGRKLDELPKGARIGTSSLRRCAQLLNYRPDFQLEALRGNLNTRMKKLVS
EQLDGIILAAAGITRMGWEDMIAEIIPFQVCLPAVGQGAISVECREDDPEILNLLKGIEHTETKAATEAERSLLRYLEGG
CQVPIGAHSEVKNNRLMLTAVVATLDGTKVIRAQGENEVNKAVELGIEVAEKLMAMGGKKILEEVRAGE

Sequences:

>Translated_309_residues
MKHKITVGSRDSALALWQTRWVVEQLEKQNPDVTFEITTMKTKGDKMLDVALAKIGDKGLFTKELEVAMLQKEIDFAVHS
LKDMPTALPEGLIIGAVCKRDNPGDALISKDGRKLDELPKGARIGTSSLRRCAQLLNYRPDFQLEALRGNLNTRMKKLVS
EQLDGIILAAAGITRMGWEDMIAEIIPFQVCLPAVGQGAISVECREDDPEILNLLKGIEHTETKAATEAERSLLRYLEGG
CQVPIGAHSEVKNNRLMLTAVVATLDGTKVIRAQGENEVNKAVELGIEVAEKLMAMGGKKILEEVRAGE
>Mature_309_residues
MKHKITVGSRDSALALWQTRWVVEQLEKQNPDVTFEITTMKTKGDKMLDVALAKIGDKGLFTKELEVAMLQKEIDFAVHS
LKDMPTALPEGLIIGAVCKRDNPGDALISKDGRKLDELPKGARIGTSSLRRCAQLLNYRPDFQLEALRGNLNTRMKKLVS
EQLDGIILAAAGITRMGWEDMIAEIIPFQVCLPAVGQGAISVECREDDPEILNLLKGIEHTETKAATEAERSLLRYLEGG
CQVPIGAHSEVKNNRLMLTAVVATLDGTKVIRAQGENEVNKAVELGIEVAEKLMAMGGKKILEEVRAGE

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=335, Percent_Identity=44.1791044776119, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI66933009, Length=335, Percent_Identity=44.1791044776119, Blast_Score=262, Evalue=3e-70,
Organism=Escherichia coli, GI48994974, Length=309, Percent_Identity=44.336569579288, Blast_Score=278, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6319996, Length=316, Percent_Identity=38.2911392405063, Blast_Score=205, Evalue=8e-54,
Organism=Drosophila melanogaster, GI20130425, Length=282, Percent_Identity=46.8085106382979, Blast_Score=231, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_DESRM (A4J6H7)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113505.1
- ProteinModelPortal:   A4J6H7
- SMR:   A4J6H7
- STRING:   A4J6H7
- GeneID:   4956247
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2163
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- PhylomeDB:   A4J6H7
- ProtClustDB:   PRK00072
- BioCyc:   DRED349161:DRED_2163-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33895; Mature: 33895

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKITVGSRDSALALWQTRWVVEQLEKQNPDVTFEITTMKTKGDKMLDVALAKIGDKGL
CCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCC
FTKELEVAMLQKEIDFAVHSLKDMPTALPEGLIIGAVCKRDNPGDALISKDGRKLDELPK
CHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCC
GARIGTSSLRRCAQLLNYRPDFQLEALRGNLNTRMKKLVSEQLDGIILAAAGITRMGWED
CCCCCHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHCCHHH
MIAEIIPFQVCLPAVGQGAISVECREDDPEILNLLKGIEHTETKAATEAERSLLRYLEGG
HHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
CQVPIGAHSEVKNNRLMLTAVVATLDGTKVIRAQGENEVNKAVELGIEVAEKLMAMGGKK
CCCCCCCCCHHCCCCEEEEHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHH
ILEEVRAGE
HHHHHHCCC
>Mature Secondary Structure
MKHKITVGSRDSALALWQTRWVVEQLEKQNPDVTFEITTMKTKGDKMLDVALAKIGDKGL
CCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCC
FTKELEVAMLQKEIDFAVHSLKDMPTALPEGLIIGAVCKRDNPGDALISKDGRKLDELPK
CHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCC
GARIGTSSLRRCAQLLNYRPDFQLEALRGNLNTRMKKLVSEQLDGIILAAAGITRMGWED
CCCCCHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHCCHHH
MIAEIIPFQVCLPAVGQGAISVECREDDPEILNLLKGIEHTETKAATEAERSLLRYLEGG
HHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
CQVPIGAHSEVKNNRLMLTAVVATLDGTKVIRAQGENEVNKAVELGIEVAEKLMAMGGKK
CCCCCCCCCHHCCCCEEEEHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHH
ILEEVRAGE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA