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The map label for this gene is hemA

Identifier: 134300010

GI number: 134300010

Start: 2368287

End: 2369681

Strand: Reverse

Name: hemA

Synonym: Dred_2164

Alternate gene names: 134300010

Gene position: 2369681-2368287 (Counterclockwise)

Preceding gene: 134300011

Following gene: 134300009

Centisome position: 65.68

GC content: 45.45

Gene sequence:

>1395_bases
ATGCTTATTGCGGTTATCGGAGTTAACCACCGCACTGCCCCCCTTGAAGTGCGAGAGAAACTTGCTTTTACTGAATGGGG
TATGAAAGATTCGTTGAAAAGACTAATGTCTTATCCAGGCATTGACGGTTGCGCCATTGTCTCTACCTGCAACAGAACAG
AAATATATATAGCCCCAATGGAGTTAGATTCAGGGATGAGTTCGGTGTGGTCTTTTTTATCTGAGAAATCAGGCCTGGAT
ATTTCTGAGATAAAAAACTTCACCTTTTGCCATACTCTGTATGATGCCATTCGACACCTGTTCCGAGTGGTCTCGGGTTT
AGACTCCATGATCTTAGGAGAAACGCAAATTTTAGGACAGGTAAAAAAGGCTTATGAACTGGCACTGGAGGCAAAAACCA
CCAATGTAGTTTTAAACACATTATTTCAGCAAGCAATTACCACTGGAAAGAGAGTGCGAACCGAGACAGGCATTGATCAA
AACCCGGTTTCCATTCCCTATGCTGCGGTTGAACTGGCAAAGCAGGGCTTTGGTACCTTAAGTGGTCGATCTGTCCTGGT
GGTGGGTGCCGGGGAGATGAGTGAATTAACGGTGGTAAATCTGGTGGCCAACGGGGTTTCCAGTGTGATTGTATCCAATC
GGTCCTATGACAGGGCGGTTCAACTGGCGGAAAAATTTGATGGAACTGCGGTTAAATTTGATCAATTGTTTCAATACATG
AACCGTGCTGATATTGTCATTTCCAGCACTGCAGCCCAGCATTACGTAATTAAAACATCTGACATGCAGAAGGTAATGGA
GCAACGGGTGGATCAACCCATCATGATGATCGATATTGCGGTTCCACGGGATATTGACCCGGAAGTTCGTAGGATTCCCA
ATGTAAAGTTGTATGATGTGGATCACTTGCAAAACGTTGTGGATGCTAACCTGGAGGAACGGCGTAGGGCAGCGGTACAG
GCCGAAGGCATTATTGAAGAGGAACTCAATGAATTTATGCGCTGGCTCTCTACCCGTTTTGTTGTTCCGACCATCACAGC
GTTAAAGAAGATGGGGGAAGAAATTAAACAACGGGAATTGGTCCGGGCCTATAACCGCCTAGGGGATCTGACGGAACGAG
AGAAAAAAATTATTGGCTCGCTGGCAAACTCCATTGTTAACCAACTTTTGCATAACCCGGTAATTAAACTTAAACAATAC
GCCCTAACACCGGAGGGACATTTATATACGGAATTAACCCAGAATATTTTTAACCTGCAAGTGGAAGGTCAAAAGCCCCA
GACTGAAATAATCCCCCCAGAAAAGAAGAAAAAGGTTGCCGGTGGTTCCACCCTTAACAGCGGTTGGGACAAGGGGATAG
CTGCAATGGTGGCAGACAGTCGATCCAGGGAATAA

Upstream 100 bases:

>100_bases
ATTCATGCAAGAAGGACTATATTTAAGAAATTAGTGGAATCTGACATTTTAGAATTACTGAGAGAGAAAAAGTACGACCA
GGTAAAGGAGCGCATTAAAA

Downstream 100 bases:

>100_bases
AAGGGAGGCTACCATGAAACATAAAATAACCGTTGGCAGCAGGGACAGTGCGCTGGCACTGTGGCAAACTCGCTGGGTAG
TTGAACAGCTGGAAAAACAA

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLIAVIGVNHRTAPLEVREKLAFTEWGMKDSLKRLMSYPGIDGCAIVSTCNRTEIYIAPMELDSGMSSVWSFLSEKSGLD
ISEIKNFTFCHTLYDAIRHLFRVVSGLDSMILGETQILGQVKKAYELALEAKTTNVVLNTLFQQAITTGKRVRTETGIDQ
NPVSIPYAAVELAKQGFGTLSGRSVLVVGAGEMSELTVVNLVANGVSSVIVSNRSYDRAVQLAEKFDGTAVKFDQLFQYM
NRADIVISSTAAQHYVIKTSDMQKVMEQRVDQPIMMIDIAVPRDIDPEVRRIPNVKLYDVDHLQNVVDANLEERRRAAVQ
AEGIIEEELNEFMRWLSTRFVVPTITALKKMGEEIKQRELVRAYNRLGDLTEREKKIIGSLANSIVNQLLHNPVIKLKQY
ALTPEGHLYTELTQNIFNLQVEGQKPQTEIIPPEKKKKVAGGSTLNSGWDKGIAAMVADSRSRE

Sequences:

>Translated_464_residues
MLIAVIGVNHRTAPLEVREKLAFTEWGMKDSLKRLMSYPGIDGCAIVSTCNRTEIYIAPMELDSGMSSVWSFLSEKSGLD
ISEIKNFTFCHTLYDAIRHLFRVVSGLDSMILGETQILGQVKKAYELALEAKTTNVVLNTLFQQAITTGKRVRTETGIDQ
NPVSIPYAAVELAKQGFGTLSGRSVLVVGAGEMSELTVVNLVANGVSSVIVSNRSYDRAVQLAEKFDGTAVKFDQLFQYM
NRADIVISSTAAQHYVIKTSDMQKVMEQRVDQPIMMIDIAVPRDIDPEVRRIPNVKLYDVDHLQNVVDANLEERRRAAVQ
AEGIIEEELNEFMRWLSTRFVVPTITALKKMGEEIKQRELVRAYNRLGDLTEREKKIIGSLANSIVNQLLHNPVIKLKQY
ALTPEGHLYTELTQNIFNLQVEGQKPQTEIIPPEKKKKVAGGSTLNSGWDKGIAAMVADSRSRE
>Mature_464_residues
MLIAVIGVNHRTAPLEVREKLAFTEWGMKDSLKRLMSYPGIDGCAIVSTCNRTEIYIAPMELDSGMSSVWSFLSEKSGLD
ISEIKNFTFCHTLYDAIRHLFRVVSGLDSMILGETQILGQVKKAYELALEAKTTNVVLNTLFQQAITTGKRVRTETGIDQ
NPVSIPYAAVELAKQGFGTLSGRSVLVVGAGEMSELTVVNLVANGVSSVIVSNRSYDRAVQLAEKFDGTAVKFDQLFQYM
NRADIVISSTAAQHYVIKTSDMQKVMEQRVDQPIMMIDIAVPRDIDPEVRRIPNVKLYDVDHLQNVVDANLEERRRAAVQ
AEGIIEEELNEFMRWLSTRFVVPTITALKKMGEEIKQRELVRAYNRLGDLTEREKKIIGSLANSIVNQLLHNPVIKLKQY
ALTPEGHLYTELTQNIFNLQVEGQKPQTEIIPPEKKKKVAGGSTLNSGWDKGIAAMVADSRSRE

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=404, Percent_Identity=37.1287128712871, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_DESRM (A4J6H8)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113506.1
- ProteinModelPortal:   A4J6H8
- SMR:   A4J6H8
- STRING:   A4J6H8
- GeneID:   4955271
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2164
- eggNOG:   COG0373
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- PhylomeDB:   A4J6H8
- ProtClustDB:   PRK00045
- BioCyc:   DRED349161:DRED_2164-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 51834; Mature: 51834

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAVIGVNHRTAPLEVREKLAFTEWGMKDSLKRLMSYPGIDGCAIVSTCNRTEIYIAPM
CEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEC
ELDSGMSSVWSFLSEKSGLDISEIKNFTFCHTLYDAIRHLFRVVSGLDSMILGETQILGQ
CCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
VKKAYELALEAKTTNVVLNTLFQQAITTGKRVRTETGIDQNPVSIPYAAVELAKQGFGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCHHHHHHHHHCCCCC
SGRSVLVVGAGEMSELTVVNLVANGVSSVIVSNRSYDRAVQLAEKFDGTAVKFDQLFQYM
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHH
NRADIVISSTAAQHYVIKTSDMQKVMEQRVDQPIMMIDIAVPRDIDPEVRRIPNVKLYDV
CCCCEEEEECCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEH
DHLQNVVDANLEERRRAAVQAEGIIEEELNEFMRWLSTRFVVPTITALKKMGEEIKQREL
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VRAYNRLGDLTEREKKIIGSLANSIVNQLLHNPVIKLKQYALTPEGHLYTELTQNIFNLQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHEEE
VEGQKPQTEIIPPEKKKKVAGGSTLNSGWDKGIAAMVADSRSRE
ECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLIAVIGVNHRTAPLEVREKLAFTEWGMKDSLKRLMSYPGIDGCAIVSTCNRTEIYIAPM
CEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEC
ELDSGMSSVWSFLSEKSGLDISEIKNFTFCHTLYDAIRHLFRVVSGLDSMILGETQILGQ
CCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
VKKAYELALEAKTTNVVLNTLFQQAITTGKRVRTETGIDQNPVSIPYAAVELAKQGFGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCHHHHHHHHHCCCCC
SGRSVLVVGAGEMSELTVVNLVANGVSSVIVSNRSYDRAVQLAEKFDGTAVKFDQLFQYM
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHH
NRADIVISSTAAQHYVIKTSDMQKVMEQRVDQPIMMIDIAVPRDIDPEVRRIPNVKLYDV
CCCCEEEEECCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEH
DHLQNVVDANLEERRRAAVQAEGIIEEELNEFMRWLSTRFVVPTITALKKMGEEIKQREL
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VRAYNRLGDLTEREKKIIGSLANSIVNQLLHNPVIKLKQYALTPEGHLYTELTQNIFNLQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHEEE
VEGQKPQTEIIPPEKKKKVAGGSTLNSGWDKGIAAMVADSRSRE
ECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA