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The map label for this gene is hemD [H]

Identifier: 134300008

GI number: 134300008

Start: 2365809

End: 2367347

Strand: Reverse

Name: hemD [H]

Synonym: Dred_2162

Alternate gene names: 134300008

Gene position: 2367347-2365809 (Counterclockwise)

Preceding gene: 134300009

Following gene: 134300007

Centisome position: 65.61

GC content: 46.72

Gene sequence:

>1539_bases
ATGAAAATGACAAAGGGTATTGTATATTTAGTAGGAGCTGGCCCAGGTGACCCGGGATTAATTACAGTAAAAGGATTGGA
GTGTATTCGTAAGGCGGATGTTCTAGTTTACGACCGTTTGGCCAGCCCAAGATTTTTGGCTTACGCCCGTCCGGATGTAG
AAATAATTTATGTGGGAAAATCACCGGACCGCCATGCCATGTCACAGGAAGATATAAACCAACTGCTGGTGGATAAGGCC
AAGGAAGGTAAGGTTGTAACTCGACTGAAGGGCGGAGACCCCTTTGTCTTTGGCAGGGGTGGTGAAGAAGCTGAATTGCT
GGTGGAAAACAACCTTCCCTTTGAAGTGGTACCGGGCATTACTTCTGCCATTTCAGTACCGGCCTATGCTGGGATACCGG
TGACCCACCGGGGTTATACCTCCACATTGGCTATTGTTACCGGTAATGAAGACCCCAATAAGGATGATTCCAGCATTGCC
TGGGATAAAATTGCTACTGGTGCAGGAACATTGATATTTTTAATGGGCATGGCTAATTTACCAGCGATTTGCACCAGATT
AATGGAATTTGGCCGTTCAACTGAAACACCGGTGGCATTAATACGCTGGGGTACCAGACCGGAACAAAGAACGCTGATTG
GTACCTTGGAGAATATTCATCAAAGGGCACTGGAAGCAAACTTTAAAAACCCGGCGGTTATTGTGGTGGGTGAGGTTGTA
AAGTTAAGAGAAAAACTAGCATGGTTCGAGAATAGACCTATGTTTGGTAAAAGGGTCATTGTCACCCGCTCCCGGGAACA
GGCCAGTGTCTTATCCTCAGCAATTGAAGCCTTGGGTGGCGAACCATGGGAGTTCCCCACCATTCAAATTGCCGAGCCTG
AGGACTTTACAATGCTGGATAGTGCCATCACCGATGTACGTTCCTATCGTTGGGTAATTTTCACCAGTGTTAACGGTGTT
CAAAGGTTCTTTGAACGTATGCGTAATAATAAAAAAGACATTCGGGATCTTAGTGATGTCCGCCTATGTGCCATTGGACC
CAAAACCCGGGAGGAATTGGAAAAAAGAGGATTGATGGTGGATTACGTCCCGGGAGAATATCGGGCCGAGGAAATTGTCG
CAGGCCTCAGGGGTAAAGTGATGCCGGGAGATCGGGTTTTATTACCCCGGGCAGACATTGCTCGTAAATTATTGCCTAAG
GCCTTGGAGGAAATGGGTGCTCTGGTACATGAAGTAGTTGCCTACCGGACAGTTTTAGGGGACGGCAATGCTAAGGCCAT
CCGTGAGAGTCTAGCCAATGGTGATATCAGTGTGGTTACATTTACTAGTTCGTCTACCGTGCAAAATTTTGTTAAACTAA
TAGCAGAAGAAGACTTACCACGATTAATGGAAAAGGTGGTAGTAGCCAGCATCGGACCCATTACATCTGACACAGCTAGG
AAATTGGGCCTGCGGGTGGATGTAGAAGCCAAGGAATATACCATTGGCGGTCTTGTACAGGCTATAACCGAGCATTTTAC
CGGTCAAAAAGATTCTTAA

Upstream 100 bases:

>100_bases
AGAACGAGGTTAACAAGGCGGTGGAATTAGGTATTGAAGTGGCTGAGAAACTAATGGCAATGGGCGGTAAAAAAATACTG
GAAGAAGTTAGGGCGGGGGA

Downstream 100 bases:

>100_bases
GATATCTTTTACGGCTGGATTAAAAATCTATAGAAAAGTGGGAGCTTTATGATTAGTATTAGTAAGCTGCTCTGTGATAC
ACAAAATTACGGAGACTCTT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKMTKGIVYLVGAGPGDPGLITVKGLECIRKADVLVYDRLASPRFLAYARPDVEIIYVGKSPDRHAMSQEDINQLLVDKA
KEGKVVTRLKGGDPFVFGRGGEEAELLVENNLPFEVVPGITSAISVPAYAGIPVTHRGYTSTLAIVTGNEDPNKDDSSIA
WDKIATGAGTLIFLMGMANLPAICTRLMEFGRSTETPVALIRWGTRPEQRTLIGTLENIHQRALEANFKNPAVIVVGEVV
KLREKLAWFENRPMFGKRVIVTRSREQASVLSSAIEALGGEPWEFPTIQIAEPEDFTMLDSAITDVRSYRWVIFTSVNGV
QRFFERMRNNKKDIRDLSDVRLCAIGPKTREELEKRGLMVDYVPGEYRAEEIVAGLRGKVMPGDRVLLPRADIARKLLPK
ALEEMGALVHEVVAYRTVLGDGNAKAIRESLANGDISVVTFTSSSTVQNFVKLIAEEDLPRLMEKVVVASIGPITSDTAR
KLGLRVDVEAKEYTIGGLVQAITEHFTGQKDS

Sequences:

>Translated_512_residues
MKMTKGIVYLVGAGPGDPGLITVKGLECIRKADVLVYDRLASPRFLAYARPDVEIIYVGKSPDRHAMSQEDINQLLVDKA
KEGKVVTRLKGGDPFVFGRGGEEAELLVENNLPFEVVPGITSAISVPAYAGIPVTHRGYTSTLAIVTGNEDPNKDDSSIA
WDKIATGAGTLIFLMGMANLPAICTRLMEFGRSTETPVALIRWGTRPEQRTLIGTLENIHQRALEANFKNPAVIVVGEVV
KLREKLAWFENRPMFGKRVIVTRSREQASVLSSAIEALGGEPWEFPTIQIAEPEDFTMLDSAITDVRSYRWVIFTSVNGV
QRFFERMRNNKKDIRDLSDVRLCAIGPKTREELEKRGLMVDYVPGEYRAEEIVAGLRGKVMPGDRVLLPRADIARKLLPK
ALEEMGALVHEVVAYRTVLGDGNAKAIRESLANGDISVVTFTSSSTVQNFVKLIAEEDLPRLMEKVVVASIGPITSDTAR
KLGLRVDVEAKEYTIGGLVQAITEHFTGQKDS
>Mature_512_residues
MKMTKGIVYLVGAGPGDPGLITVKGLECIRKADVLVYDRLASPRFLAYARPDVEIIYVGKSPDRHAMSQEDINQLLVDKA
KEGKVVTRLKGGDPFVFGRGGEEAELLVENNLPFEVVPGITSAISVPAYAGIPVTHRGYTSTLAIVTGNEDPNKDDSSIA
WDKIATGAGTLIFLMGMANLPAICTRLMEFGRSTETPVALIRWGTRPEQRTLIGTLENIHQRALEANFKNPAVIVVGEVV
KLREKLAWFENRPMFGKRVIVTRSREQASVLSSAIEALGGEPWEFPTIQIAEPEDFTMLDSAITDVRSYRWVIFTSVNGV
QRFFERMRNNKKDIRDLSDVRLCAIGPKTREELEKRGLMVDYVPGEYRAEEIVAGLRGKVMPGDRVLLPRADIARKLLPK
ALEEMGALVHEVVAYRTVLGDGNAKAIRESLANGDISVVTFTSSSTVQNFVKLIAEEDLPRLMEKVVVASIGPITSDTAR
KLGLRVDVEAKEYTIGGLVQAITEHFTGQKDS

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=248, Percent_Identity=46.7741935483871, Blast_Score=231, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=30.2127659574468, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 56335; Mature: 56335

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTKGIVYLVGAGPGDPGLITVKGLECIRKADVLVYDRLASPRFLAYARPDVEIIYVGK
CCCCCCEEEEEECCCCCCCEEEEHHHHHHHHCCEEEEECCCCCCEEEEECCCEEEEEECC
SPDRHAMSQEDINQLLVDKAKEGKVVTRLKGGDPFVFGRGGEEAELLVENNLPFEVVPGI
CCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCEEEEECCCCEEECCCC
TSAISVPAYAGIPVTHRGYTSTLAIVTGNEDPNKDDSSIAWDKIATGAGTLIFLMGMANL
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCCHHHHHHHHCCH
PAICTRLMEFGRSTETPVALIRWGTRPEQRTLIGTLENIHQRALEANFKNPAVIVVGEVV
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
KLREKLAWFENRPMFGKRVIVTRSREQASVLSSAIEALGGEPWEFPTIQIAEPEDFTMLD
HHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
SAITDVRSYRWVIFTSVNGVQRFFERMRNNKKDIRDLSDVRLCAIGPKTREELEKRGLMV
HHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHCEEEEECCHHHHHHHHCCCEE
DYVPGEYRAEEIVAGLRGKVMPGDRVLLPRADIARKLLPKALEEMGALVHEVVAYRTVLG
EECCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGNAKAIRESLANGDISVVTFTSSSTVQNFVKLIAEEDLPRLMEKVVVASIGPITSDTAR
CCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHH
KLGLRVDVEAKEYTIGGLVQAITEHFTGQKDS
HCCCEEECCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKMTKGIVYLVGAGPGDPGLITVKGLECIRKADVLVYDRLASPRFLAYARPDVEIIYVGK
CCCCCCEEEEEECCCCCCCEEEEHHHHHHHHCCEEEEECCCCCCEEEEECCCEEEEEECC
SPDRHAMSQEDINQLLVDKAKEGKVVTRLKGGDPFVFGRGGEEAELLVENNLPFEVVPGI
CCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCEEEEECCCCEEECCCC
TSAISVPAYAGIPVTHRGYTSTLAIVTGNEDPNKDDSSIAWDKIATGAGTLIFLMGMANL
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCCHHHHHHHHCCH
PAICTRLMEFGRSTETPVALIRWGTRPEQRTLIGTLENIHQRALEANFKNPAVIVVGEVV
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
KLREKLAWFENRPMFGKRVIVTRSREQASVLSSAIEALGGEPWEFPTIQIAEPEDFTMLD
HHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
SAITDVRSYRWVIFTSVNGVQRFFERMRNNKKDIRDLSDVRLCAIGPKTREELEKRGLMV
HHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHCEEEEECCHHHHHHHHCCCEE
DYVPGEYRAEEIVAGLRGKVMPGDRVLLPRADIARKLLPKALEEMGALVHEVVAYRTVLG
EECCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGNAKAIRESLANGDISVVTFTSSSTVQNFVKLIAEEDLPRLMEKVVVASIGPITSDTAR
CCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHH
KLGLRVDVEAKEYTIGGLVQAITEHFTGQKDS
HCCCEEECCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]