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The map label for this gene is truB [H]

Identifier: 134299801

GI number: 134299801

Start: 2144641

End: 2145573

Strand: Reverse

Name: truB [H]

Synonym: Dred_1954

Alternate gene names: 134299801

Gene position: 2145573-2144641 (Counterclockwise)

Preceding gene: 134299802

Following gene: 134299800

Centisome position: 59.47

GC content: 44.69

Gene sequence:

>933_bases
GTGGATGGGGTCATCAATATCTTAAAGCCTCCGGGAATGACATCCCACGATGTTGTATTCTTTGTAAGACGGGTTACTGG
GGTGAAGAAATGTGGGCATACTGGTACCCTTGACCCTGGAGCATCGGGTGTTCTCCCAGTATGTGTAGGCAAAGCTACAC
GACTAGCTAGGTTTGTTACAGATGGAGACAAGACTTATAGGGCTGAAATAACTCTGGGAATTGAAACATCCAGTCAGGAT
GCCTTTGGCAATGTTATTAGCCGCATCGATGCATCGGCCATAAAATTGGATGACTTTTCCAGTGAATTACGCAAATTCCA
GGGAGAGATTAAACAAACCCCGCCTATGGCCTCAGCCATTAAGGTTAAGGGCAAAAAACTCTATGAACTTGAACGGGAAG
GTATAATAGTAGATGTTCCTACTAGAACGGTTACCATATATCAGGTGAATATTGTAGACAGTTGGGATTGGGGAACCCCA
AACCCCAGGGTATTGTTTGATGTGGCTTGTTCAAAGGGGACATATGTGCGTACCCTGTGTTCGGATATCGGCAAGGAACT
GGGTTGTGGCGCCTATATGTCCTTTTTGTTACGTTCCCGGGTAGCCGCCTTTGGCTTGCACAATGCTTATACATTGGAGG
AAATTGCAGCTTTAGCTGACAAAAGCCAACTAGAGAGTGCAGTAATCCCGATGGCTAAGGCTGTTCAGCATTTACCCATA
GTTGAAATTCATCCTTCTGCGCAAAAATCAGTATGCTCCGGAGCAACCCTGTATCCGGCGGGTGTAAGAGTAGGATTAGA
ATACCTAAAGCCCGAAGGTCTTGTTCAAGTTCAACTAAATCACGAATTGTTAGGTATTTATAAGCCCGTTTTAGATGTAA
ACGATTTTGGGGAACGCATAATTCTAAGACCCGAAGTGGTTTTTGCTTCATAA

Upstream 100 bases:

>100_bases
GGACATGTTCGAGCCTCAGGGTGTACCATTCAAGGTACACTTCAGGAAGTTAAGGAAGTCGTTCTCAAGGAAGCCCAAGA
AATATTCCGGGGGTGTTAAG

Downstream 100 bases:

>100_bases
ATACAGAATGATTTTATGGATCTACCGGAATTCTGTGAGGTATTATATTAAATAGGGGCAGAATGTTGCAGTTGGCAGGG
GGTAAGAGAGTTGCGTGTTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDGVINILKPPGMTSHDVVFFVRRVTGVKKCGHTGTLDPGASGVLPVCVGKATRLARFVTDGDKTYRAEITLGIETSSQD
AFGNVISRIDASAIKLDDFSSELRKFQGEIKQTPPMASAIKVKGKKLYELEREGIIVDVPTRTVTIYQVNIVDSWDWGTP
NPRVLFDVACSKGTYVRTLCSDIGKELGCGAYMSFLLRSRVAAFGLHNAYTLEEIAALADKSQLESAVIPMAKAVQHLPI
VEIHPSAQKSVCSGATLYPAGVRVGLEYLKPEGLVQVQLNHELLGIYKPVLDVNDFGERIILRPEVVFAS

Sequences:

>Translated_310_residues
MDGVINILKPPGMTSHDVVFFVRRVTGVKKCGHTGTLDPGASGVLPVCVGKATRLARFVTDGDKTYRAEITLGIETSSQD
AFGNVISRIDASAIKLDDFSSELRKFQGEIKQTPPMASAIKVKGKKLYELEREGIIVDVPTRTVTIYQVNIVDSWDWGTP
NPRVLFDVACSKGTYVRTLCSDIGKELGCGAYMSFLLRSRVAAFGLHNAYTLEEIAALADKSQLESAVIPMAKAVQHLPI
VEIHPSAQKSVCSGATLYPAGVRVGLEYLKPEGLVQVQLNHELLGIYKPVLDVNDFGERIILRPEVVFAS
>Mature_310_residues
MDGVINILKPPGMTSHDVVFFVRRVTGVKKCGHTGTLDPGASGVLPVCVGKATRLARFVTDGDKTYRAEITLGIETSSQD
AFGNVISRIDASAIKLDDFSSELRKFQGEIKQTPPMASAIKVKGKKLYELEREGIIVDVPTRTVTIYQVNIVDSWDWGTP
NPRVLFDVACSKGTYVRTLCSDIGKELGCGAYMSFLLRSRVAAFGLHNAYTLEEIAALADKSQLESAVIPMAKAVQHLPI
VEIHPSAQKSVCSGATLYPAGVRVGLEYLKPEGLVQVQLNHELLGIYKPVLDVNDFGERIILRPEVVFAS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=306, Percent_Identity=31.6993464052288, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4503337, Length=271, Percent_Identity=29.8892988929889, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI215599015, Length=271, Percent_Identity=29.8892988929889, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI2367200, Length=286, Percent_Identity=32.5174825174825, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17553978, Length=269, Percent_Identity=28.6245353159851, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=270, Percent_Identity=30.7407407407407, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=39.8496240601504, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364189, Length=270, Percent_Identity=27.4074074074074, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281364187, Length=270, Percent_Identity=27.4074074074074, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281364185, Length=270, Percent_Identity=27.4074074074074, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281364183, Length=270, Percent_Identity=27.4074074074074, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI62471759, Length=270, Percent_Identity=27.4074074074074, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17975520, Length=270, Percent_Identity=27.4074074074074, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33734; Mature: 33734

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVINILKPPGMTSHDVVFFVRRVTGVKKCGHTGTLDPGASGVLPVCVGKATRLARFVT
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHHHHHHC
DGDKTYRAEITLGIETSSQDAFGNVISRIDASAIKLDDFSSELRKFQGEIKQTPPMASAI
CCCCEEEEEEEEEEECCCCHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHCCCCCCHHE
KVKGKKLYELEREGIIVDVPTRTVTIYQVNIVDSWDWGTPNPRVLFDVACSKGTYVRTLC
EECCCHHEEECCCCEEEECCCCEEEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHH
SDIGKELGCGAYMSFLLRSRVAAFGLHNAYTLEEIAALADKSQLESAVIPMAKAVQHLPI
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
VEIHPSAQKSVCSGATLYPAGVRVGLEYLKPEGLVQVQLNHELLGIYKPVLDVNDFGERI
EEECCCHHHHHHCCCEECCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEE
ILRPEVVFAS
EECCEEEECC
>Mature Secondary Structure
MDGVINILKPPGMTSHDVVFFVRRVTGVKKCGHTGTLDPGASGVLPVCVGKATRLARFVT
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHHHHHHC
DGDKTYRAEITLGIETSSQDAFGNVISRIDASAIKLDDFSSELRKFQGEIKQTPPMASAI
CCCCEEEEEEEEEEECCCCHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHCCCCCCHHE
KVKGKKLYELEREGIIVDVPTRTVTIYQVNIVDSWDWGTPNPRVLFDVACSKGTYVRTLC
EECCCHHEEECCCCEEEECCCCEEEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHH
SDIGKELGCGAYMSFLLRSRVAAFGLHNAYTLEEIAALADKSQLESAVIPMAKAVQHLPI
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
VEIHPSAQKSVCSGATLYPAGVRVGLEYLKPEGLVQVQLNHELLGIYKPVLDVNDFGERI
EEECCCHHHHHHCCCEECCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEE
ILRPEVVFAS
EECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA