Click here to switch to the map view.

The map label for this gene is nudF [H]

Identifier: 134299751

GI number: 134299751

Start: 2089550

End: 2090086

Strand: Reverse

Name: nudF [H]

Synonym: Dred_1901

Alternate gene names: 134299751

Gene position: 2090086-2089550 (Counterclockwise)

Preceding gene: 134299752

Following gene: 134299748

Centisome position: 57.93

GC content: 43.02

Gene sequence:

>537_bases
GTGAAGGATTTAGTGGAAACAACCATAGCCTCAAAGCTGGTCTATGAAGGAAAAATTCTCAATCTACGGGTGGATCAAGT
AACTCTGCCAAATGGTAGGGAAGGCAGCCGGGAAGTTGTGGAGTTTTCCCAGGCGGTGGCTGTGGTGGCCGTGAAGGAAG
ACAATAAAGTTTTACTTGTTAAACAATATCGTTATCCAGTGGGGGAAGTACTGATGGAATTACCTGCTGGGAAGATGGAT
CAAGATGAAAATCCGGAACAATGTGCCCTCCGGGAATTGCAGGAAGAAACTGGTTATAAGCCTAGATCCATTCAAAAAAT
CTGTGATTTTTATACAACGCCTGGTTTTTCTTCTGAACGAATGCATCTATTTTTGGCTACAGGACTTACTGAAGGGGAGC
AAAGCCCCGATGAAGATGAATTTGTAAAAGTTGAAGAGGTTCCCTTTGATCAAGCTATTCAGATGATATTTGAGGGGAAA
ATCCAGGATGGCAAAACCATTGCAGGCCTCCTAGCGGTTGAAAAGAAATATAGATAG

Upstream 100 bases:

>100_bases
CCAAGATATCTATTGAATACGAAATAGATTTTTCCAGAAGGAAAAATATTCTCCAACGGGAATAATGCTTAGGTAAAACG
AATAGAGGAGTTGTGTAATT

Downstream 100 bases:

>100_bases
TTAAAAAAGCCCCCGCTCATTCCACTTTCGGAAGGAGCGGGGGCTTTCTCCATTTAATCAATGACTCTTATTGTTTAAAG
ACAAAGATCTCATATTTAAT

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MKDLVETTIASKLVYEGKILNLRVDQVTLPNGREGSREVVEFSQAVAVVAVKEDNKVLLVKQYRYPVGEVLMELPAGKMD
QDENPEQCALRELQEETGYKPRSIQKICDFYTTPGFSSERMHLFLATGLTEGEQSPDEDEFVKVEEVPFDQAIQMIFEGK
IQDGKTIAGLLAVEKKYR

Sequences:

>Translated_178_residues
MKDLVETTIASKLVYEGKILNLRVDQVTLPNGREGSREVVEFSQAVAVVAVKEDNKVLLVKQYRYPVGEVLMELPAGKMD
QDENPEQCALRELQEETGYKPRSIQKICDFYTTPGFSSERMHLFLATGLTEGEQSPDEDEFVKVEEVPFDQAIQMIFEGK
IQDGKTIAGLLAVEKKYR
>Mature_178_residues
MKDLVETTIASKLVYEGKILNLRVDQVTLPNGREGSREVVEFSQAVAVVAVKEDNKVLLVKQYRYPVGEVLMELPAGKMD
QDENPEQCALRELQEETGYKPRSIQKICDFYTTPGFSSERMHLFLATGLTEGEQSPDEDEFVKVEEVPFDQAIQMIFEGK
IQDGKTIAGLLAVEKKYR

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI37594464, Length=165, Percent_Identity=31.5151515151515, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788810, Length=143, Percent_Identity=34.965034965035, Blast_Score=77, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20120; Mature: 20120

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLVETTIASKLVYEGKILNLRVDQVTLPNGREGSREVVEFSQAVAVVAVKEDNKVLLV
CCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHHEEEEEEECCCCEEEE
KQYRYPVGEVLMELPAGKMDQDENPEQCALRELQEETGYKPRSIQKICDFYTTPGFSSER
EECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
MHLFLATGLTEGEQSPDEDEFVKVEEVPFDQAIQMIFEGKIQDGKTIAGLLAVEKKYR
EEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKDLVETTIASKLVYEGKILNLRVDQVTLPNGREGSREVVEFSQAVAVVAVKEDNKVLLV
CCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHHEEEEEEECCCCEEEE
KQYRYPVGEVLMELPAGKMDQDENPEQCALRELQEETGYKPRSIQKICDFYTTPGFSSER
EECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
MHLFLATGLTEGEQSPDEDEFVKVEEVPFDQAIQMIFEGKIQDGKTIAGLLAVEKKYR
EEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]