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The map label for this gene is ybfF [C]

Identifier: 134299748

GI number: 134299748

Start: 2086390

End: 2087136

Strand: Reverse

Name: ybfF [C]

Synonym: Dred_1898

Alternate gene names: 134299748

Gene position: 2087136-2086390 (Counterclockwise)

Preceding gene: 134299751

Following gene: 134299747

Centisome position: 57.85

GC content: 38.55

Gene sequence:

>747_bases
ATGATTACCTATCATCCCAAATTATCTATGATTATTCCCTTTTATAACGAAGAAGAAAATGTTGAAAGGGTGGTACTGGA
ACTTCAACAAGTGTTAGAAAAAGAACAAATCAAATATGAATTAATTTTGGTTAATAATGGTTCAACAGACAAGACCGGAT
GGCTTTTGGATGACTTAGCTAAGAGGAATTCTGGTATCAAAGTAATTACAGTATCCATAAATCATGGTTATGGCTGGGGA
ATCATCAACGGTCTACGCTGGGCCTGTGGTGATTACCTGGGCTTCATGGGGGGAGATGGTCAGATTGATCCCTCTGATGT
TTCAAAGGTTTTCAATACTTTACTAGAGGGGAAATATCACCTTTGCAAGGTTAATCGATGCCAGCGGGAGGATGGCCCAA
TTCGGAAATTGACATCACACATATTTAATCGAGCATTTGTATATACATTTAAGGTAAATGTTGGGGATATCAATGGATCA
CCCAAAATCATGAGTCGTACATGTTACGAACAATTAAATCTTTGTGCAAAGGATTGGTTTTTGGATGCGGAAGTTATACT
AAAATCCCAATACTTAAATTTTTCCATAGGAGAAGTTCCGGTAGCATTTCACCGTCGTATGGGTGGTCATTCCAGTGTTC
GAATAAGAACGGTGTGGGAGTTTATCAAGAATATGGCTTTCTACCGTAAAAGAGGTGTCTTTTATGAAAGTAGTGATTTT
ATGTGGGGGAAAAGGGACCCGGCTTAA

Upstream 100 bases:

>100_bases
ATGGTTTTATTTTTCATATATTTGCTAGTATAACCAGTATAGAACTGAAAAATGTTGGTCAAACTCTATTGGGAAAATAG
TGAGATAAAGGAGCAACCCT

Downstream 100 bases:

>100_bases
AGAAGAAACGGCTTTCAGACCGAAGCCACTGGTAACCGTGGGGAAAAAACCAATACTTTGGCATATCATGAAATATTATT
CCCATTACGGTTTCAAAGAT

Product: glycosyl transferase family protein

Products: GDP; dolichyl D-mannosyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MITYHPKLSMIIPFYNEEENVERVVLELQQVLEKEQIKYELILVNNGSTDKTGWLLDDLAKRNSGIKVITVSINHGYGWG
IINGLRWACGDYLGFMGGDGQIDPSDVSKVFNTLLEGKYHLCKVNRCQREDGPIRKLTSHIFNRAFVYTFKVNVGDINGS
PKIMSRTCYEQLNLCAKDWFLDAEVILKSQYLNFSIGEVPVAFHRRMGGHSSVRIRTVWEFIKNMAFYRKRGVFYESSDF
MWGKRDPA

Sequences:

>Translated_248_residues
MITYHPKLSMIIPFYNEEENVERVVLELQQVLEKEQIKYELILVNNGSTDKTGWLLDDLAKRNSGIKVITVSINHGYGWG
IINGLRWACGDYLGFMGGDGQIDPSDVSKVFNTLLEGKYHLCKVNRCQREDGPIRKLTSHIFNRAFVYTFKVNVGDINGS
PKIMSRTCYEQLNLCAKDWFLDAEVILKSQYLNFSIGEVPVAFHRRMGGHSSVRIRTVWEFIKNMAFYRKRGVFYESSDF
MWGKRDPA
>Mature_248_residues
MITYHPKLSMIIPFYNEEENVERVVLELQQVLEKEQIKYELILVNNGSTDKTGWLLDDLAKRNSGIKVITVSINHGYGWG
IINGLRWACGDYLGFMGGDGQIDPSDVSKVFNTLLEGKYHLCKVNRCQREDGPIRKLTSHIFNRAFVYTFKVNVGDINGS
PKIMSRTCYEQLNLCAKDWFLDAEVILKSQYLNFSIGEVPVAFHRRMGGHSSVRIRTVWEFIKNMAFYRKRGVFYESSDF
MWGKRDPA

Specific function: Unknown

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family. GtrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503363, Length=228, Percent_Identity=25.8771929824561, Blast_Score=85, Evalue=5e-17,
Organism=Escherichia coli, GI1788588, Length=215, Percent_Identity=26.9767441860465, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787259, Length=111, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71999402, Length=237, Percent_Identity=24.8945147679325, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585265, Length=236, Percent_Identity=24.1525423728814, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.4.1.83

Molecular weight: Translated: 28623; Mature: 28623

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITYHPKLSMIIPFYNEEENVERVVLELQQVLEKEQIKYELILVNNGSTDKTGWLLDDLA
CEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHH
KRNSGIKVITVSINHGYGWGIINGLRWACGDYLGFMGGDGQIDPSDVSKVFNTLLEGKYH
HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEE
LCKVNRCQREDGPIRKLTSHIFNRAFVYTFKVNVGDINGSPKIMSRTCYEQLNLCAKDWF
EEECCHHCCCCCHHHHHHHHHHCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
LDAEVILKSQYLNFSIGEVPVAFHRRMGGHSSVRIRTVWEFIKNMAFYRKRGVFYESSDF
CCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCC
MWGKRDPA
CCCCCCCC
>Mature Secondary Structure
MITYHPKLSMIIPFYNEEENVERVVLELQQVLEKEQIKYELILVNNGSTDKTGWLLDDLA
CEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHH
KRNSGIKVITVSINHGYGWGIINGLRWACGDYLGFMGGDGQIDPSDVSKVFNTLLEGKYH
HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEE
LCKVNRCQREDGPIRKLTSHIFNRAFVYTFKVNVGDINGSPKIMSRTCYEQLNLCAKDWF
EEECCHHCCCCCHHHHHHHHHHCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
LDAEVILKSQYLNFSIGEVPVAFHRRMGGHSSVRIRTVWEFIKNMAFYRKRGVFYESSDF
CCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCC
MWGKRDPA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPmannose; dolichyl phosphate

Specific reaction: GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]