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The map label for this gene is engD [H]

Identifier: 134299143

GI number: 134299143

Start: 1366100

End: 1367203

Strand: Reverse

Name: engD [H]

Synonym: Dred_1280

Alternate gene names: 134299143

Gene position: 1367203-1366100 (Counterclockwise)

Preceding gene: 134299178

Following gene: 134299121

Centisome position: 37.89

GC content: 42.75

Gene sequence:

>1104_bases
ATGCCATTAAAATGTGGAATTGTCGGATTACCCAATGTTGGAAAATCCACCTTGTTTAATGCTATTACACAAGCAGGGGC
TGAATCTGCAAACTATCCGTTTTGTACCATCGAACCCAATGTTGGGGTTGTAACCGTACCTGATCATCGTCTGGACAAGC
TAACTGAGCTGGTGATACCCAAGAAAACCATCCCAACCACCTTTGAATTTGTGGATATTGCCGGTCTGGTGAAAGGTGCC
AGCCGTGGTGAAGGATTGGGAAATAAGTTTTTATCTCATATCAGAGAAGTGGATGCCATTGCTCATGTTGTACGTTGCTT
CATTGATGATGATATCACGCATGTTTCCGGTAAAATTGATCCTATAAATGATATTGAAGTGATCAATTTGGAACTGGTTT
TAGCTGATTTAGAAAGTATGGAACGTCGTCAGGAGCGCCTGGCCAGCAAACTTAAGTCTCAAGACAAACAGGCAAAGATT
GAGTATCAGTTGATTCAAAGGTTAAAGGATGAGGCCCTGGCCCAGGGTAAACCGGCCAGAAGCTTGGAGTTTACCGATGA
AGAAATGAAAATTGTAAAATTCTTTCATCTACTAACCATTAAGCCCATTATCTATGTGACCAATGTGGGTGAAAATGACC
TGCCTGACCTTGAGAAGAATTCCTATATTCAGCAAGTCAAAGAATATGCAGTACAAGAAAATGCCACAGTGGTACCTGTC
TCTGCTCAATTGGAGTCTGAAATTGCTGAGTTGCAAGGGGAAGAGAAAAGGCTTTTTCTAGAGGAATTAGGTTTAGATGA
ATCTGGGCTAGATAAGCTCATTCGAGCCTCCTATAATCTGCTGGGACTTCGTACTTACTTTACCGCAGGTGTGCAAGAGG
TGCGCGCTTGGACTATCACTGCAGGTACCAAAGCACCTCAGGCAGCTGGCGTTATTCACACTGATTTTGAGCGTGGTTTT
ATCAGAGCCGAGGTTGTTTCTTATGATGATTTAGTTACAGCAGGCTCTATGGCCAAGGCCAGAGAAGCTGGAAAGCTTCG
TTTAGAAGGTAAAGATTATGTGGTGCAAGATGGGGATATTATGCACTTCCGATTTAATGTTTAA

Upstream 100 bases:

>100_bases
AAATATATAAAATAGGATTTATTATTTTAGCTTGCGAAACAAAAAAATTATTACTATACTTTTAAGAGTGTGTTTAAATT
TTTATGCAAGGAGTCATCTC

Downstream 100 bases:

>100_bases
TTCGAGCGAACAATAAAATACCGTAGCATTTTCCGCTACGGTATTTTATTTTTATTTCTTTAACTCTCTGGCATACAGTG
CTGCTCCCAATGCGCCGGCC

Product: GTP-binding protein YchF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MPLKCGIVGLPNVGKSTLFNAITQAGAESANYPFCTIEPNVGVVTVPDHRLDKLTELVIPKKTIPTTFEFVDIAGLVKGA
SRGEGLGNKFLSHIREVDAIAHVVRCFIDDDITHVSGKIDPINDIEVINLELVLADLESMERRQERLASKLKSQDKQAKI
EYQLIQRLKDEALAQGKPARSLEFTDEEMKIVKFFHLLTIKPIIYVTNVGENDLPDLEKNSYIQQVKEYAVQENATVVPV
SAQLESEIAELQGEEKRLFLEELGLDESGLDKLIRASYNLLGLRTYFTAGVQEVRAWTITAGTKAPQAAGVIHTDFERGF
IRAEVVSYDDLVTAGSMAKAREAGKLRLEGKDYVVQDGDIMHFRFNV

Sequences:

>Translated_367_residues
MPLKCGIVGLPNVGKSTLFNAITQAGAESANYPFCTIEPNVGVVTVPDHRLDKLTELVIPKKTIPTTFEFVDIAGLVKGA
SRGEGLGNKFLSHIREVDAIAHVVRCFIDDDITHVSGKIDPINDIEVINLELVLADLESMERRQERLASKLKSQDKQAKI
EYQLIQRLKDEALAQGKPARSLEFTDEEMKIVKFFHLLTIKPIIYVTNVGENDLPDLEKNSYIQQVKEYAVQENATVVPV
SAQLESEIAELQGEEKRLFLEELGLDESGLDKLIRASYNLLGLRTYFTAGVQEVRAWTITAGTKAPQAAGVIHTDFERGF
IRAEVVSYDDLVTAGSMAKAREAGKLRLEGKDYVVQDGDIMHFRFNV
>Mature_366_residues
PLKCGIVGLPNVGKSTLFNAITQAGAESANYPFCTIEPNVGVVTVPDHRLDKLTELVIPKKTIPTTFEFVDIAGLVKGAS
RGEGLGNKFLSHIREVDAIAHVVRCFIDDDITHVSGKIDPINDIEVINLELVLADLESMERRQERLASKLKSQDKQAKIE
YQLIQRLKDEALAQGKPARSLEFTDEEMKIVKFFHLLTIKPIIYVTNVGENDLPDLEKNSYIQQVKEYAVQENATVVPVS
AQLESEIAELQGEEKRLFLEELGLDESGLDKLIRASYNLLGLRTYFTAGVQEVRAWTITAGTKAPQAAGVIHTDFERGFI
RAEVVSYDDLVTAGSMAKAREAGKLRLEGKDYVVQDGDIMHFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=47.4254742547426, Blast_Score=334, Evalue=7e-92,
Organism=Homo sapiens, GI58761502, Length=216, Percent_Identity=43.0555555555556, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI24308117, Length=168, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI111955139, Length=233, Percent_Identity=27.4678111587983, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787454, Length=367, Percent_Identity=58.3106267029973, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1789574, Length=127, Percent_Identity=34.6456692913386, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=43.8692098092643, Blast_Score=319, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6319499, Length=373, Percent_Identity=44.5040214477212, Blast_Score=272, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6321773, Length=382, Percent_Identity=38.2198952879581, Blast_Score=235, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6321649, Length=244, Percent_Identity=33.6065573770492, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=44.0217391304348, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=44.0217391304348, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=44.0217391304348, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24640879, Length=327, Percent_Identity=41.8960244648318, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24585318, Length=200, Percent_Identity=29, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129375, Length=169, Percent_Identity=31.3609467455621, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40786; Mature: 40655

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKCGIVGLPNVGKSTLFNAITQAGAESANYPFCTIEPNVGVVTVPDHRLDKLTELVIP
CCCEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHCC
KKTIPTTFEFVDIAGLVKGASRGEGLGNKFLSHIREVDAIAHVVRCFIDDDITHVSGKID
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCC
PINDIEVINLELVLADLESMERRQERLASKLKSQDKQAKIEYQLIQRLKDEALAQGKPAR
CCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SLEFTDEEMKIVKFFHLLTIKPIIYVTNVGENDLPDLEKNSYIQQVKEYAVQENATVVPV
CCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
SAQLESEIAELQGEEKRLFLEELGLDESGLDKLIRASYNLLGLRTYFTAGVQEVRAWTIT
HHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
AGTKAPQAAGVIHTDFERGFIRAEVVSYDDLVTAGSMAKAREAGKLRLEGKDYVVQDGDI
CCCCCCCCCCCEEECCCCCEEEEEEECHHHHHCCCCHHHHHHCCCEEECCCCEEEECCCE
MHFRFNV
EEEEECC
>Mature Secondary Structure 
PLKCGIVGLPNVGKSTLFNAITQAGAESANYPFCTIEPNVGVVTVPDHRLDKLTELVIP
CCEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHCC
KKTIPTTFEFVDIAGLVKGASRGEGLGNKFLSHIREVDAIAHVVRCFIDDDITHVSGKID
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCC
PINDIEVINLELVLADLESMERRQERLASKLKSQDKQAKIEYQLIQRLKDEALAQGKPAR
CCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SLEFTDEEMKIVKFFHLLTIKPIIYVTNVGENDLPDLEKNSYIQQVKEYAVQENATVVPV
CCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
SAQLESEIAELQGEEKRLFLEELGLDESGLDKLIRASYNLLGLRTYFTAGVQEVRAWTIT
HHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
AGTKAPQAAGVIHTDFERGFIRAEVVSYDDLVTAGSMAKAREAGKLRLEGKDYVVQDGDI
CCCCCCCCCCCEEECCCCCEEEEEEECHHHHHCCCCHHHHHHCCCEEECCCCEEEECCCE
MHFRFNV
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]