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The map label for this gene is proB [H]

Identifier: 134299033

GI number: 134299033

Start: 1243396

End: 1244496

Strand: Reverse

Name: proB [H]

Synonym: Dred_1170

Alternate gene names: 134299033

Gene position: 1244496-1243396 (Counterclockwise)

Preceding gene: 134299038

Following gene: 134299032

Centisome position: 34.49

GC content: 44.14

Gene sequence:

>1101_bases
TTGGAACAGCATAGAAGGATCGTTATAAAAATCGGTAGCAGTAGCCTGACGAATCGTGAAAAATTACTGGATGAATACAA
GTTAAAAGCCCTCGCAAGTGCTGTGGCAAAGCTTTTTAACCAAGGTTGGCAACCAATTCTTGTATCTTCGGGAGCAGTTG
CTGCGGGATTGGGTCACCTCGGTTGGAACTGCACTCATTTAACGATGCCGGAAAGACAGGCGGCCGCTGCCGTTGGTCAA
GGGCTATTGATGGAAAAGTACAATGAGTTATTTCAATCCTTGGGCATCAAGGTTGGGCAAATTCTCCTTACCCGTGCAGA
TTTTGCGGATCGTAAACGTTACCTAAATGCCCGCAACACACTAAATGTACTGTTAAATCGTCATGTTTTGCCCATTGTCA
ATGAGAATGATACCGTAACCTTTGCCGAGATCCGTTTTGGCGATAACGATACCCTAAGTGCATTGGTTGCAGGTTTAGTA
GAAGCTGAACTCCTTATTCTTCTTACAGACATCGATGGACTCTATAACGGTGACCCCAGGAAGGACCCTAATGCAAAGTT
AATTCCGGTAGTTCAGCATATTGATGATAGCATAATTAAATTAGCTGGTGATAGTGGTAGTACCGTGGGTACCGGAGGTA
TGAAAACAAAACTAGCTGCTGCTCGTATTGCTGCCCGCAGTGGCATAAGAACCGTGGTGGCCAGTGCAAAAGAACCGTCA
ATTTTATCAGGCATTGCTACAGGCGAGAAACTGGGTACTTCCTTTGAACCCTCTGATCATACTCTGAGAAGTCGCAAACA
GTGGATTGCCTATGGAAGTATAACACGCGGAACTTTGCTCATCGACAAAGGCGCAGTAAAGGCCCTTACAGAAAGACATA
AAAGTTTACTGGCCGTTGGTATAATTGGTATAAAGGGAGATTTCACTGCAGGAAGCGTTGTATCAGTTGCTGATGAACAG
GGTGTTGTCCTGGCAAAGGGTATAACAAACCTTTCTTCTGAGGATTTACGCCTTGTTAACAAAAATACACGTCACGGATT
GGAAGTTATTAATCGGGACTGGTTAGTCTGTGAAAGGGGTTTTTCTATATGTCAAATGTAA

Upstream 100 bases:

>100_bases
GTACCACGAAAGAATCCTTTCGTCCTTTGGGGGCGGAGGTTTTTTTATTTTTAGAGCTGTTTTTATTTAGAGTATCGAAA
AACCAGAGGAGGCTACAAGA

Downstream 100 bases:

>100_bases
AGAATCTGGCTAAAAAAGCTAAAATCGCCGCCAGGACACTATCTGTTTTGGATACAGAAGCTAAAAATTCTGCTTTAGAA
GCCATTGCTTCTCAGTTACT

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MEQHRRIVIKIGSSSLTNREKLLDEYKLKALASAVAKLFNQGWQPILVSSGAVAAGLGHLGWNCTHLTMPERQAAAAVGQ
GLLMEKYNELFQSLGIKVGQILLTRADFADRKRYLNARNTLNVLLNRHVLPIVNENDTVTFAEIRFGDNDTLSALVAGLV
EAELLILLTDIDGLYNGDPRKDPNAKLIPVVQHIDDSIIKLAGDSGSTVGTGGMKTKLAAARIAARSGIRTVVASAKEPS
ILSGIATGEKLGTSFEPSDHTLRSRKQWIAYGSITRGTLLIDKGAVKALTERHKSLLAVGIIGIKGDFTAGSVVSVADEQ
GVVLAKGITNLSSEDLRLVNKNTRHGLEVINRDWLVCERGFSICQM

Sequences:

>Translated_366_residues
MEQHRRIVIKIGSSSLTNREKLLDEYKLKALASAVAKLFNQGWQPILVSSGAVAAGLGHLGWNCTHLTMPERQAAAAVGQ
GLLMEKYNELFQSLGIKVGQILLTRADFADRKRYLNARNTLNVLLNRHVLPIVNENDTVTFAEIRFGDNDTLSALVAGLV
EAELLILLTDIDGLYNGDPRKDPNAKLIPVVQHIDDSIIKLAGDSGSTVGTGGMKTKLAAARIAARSGIRTVVASAKEPS
ILSGIATGEKLGTSFEPSDHTLRSRKQWIAYGSITRGTLLIDKGAVKALTERHKSLLAVGIIGIKGDFTAGSVVSVADEQ
GVVLAKGITNLSSEDLRLVNKNTRHGLEVINRDWLVCERGFSICQM
>Mature_366_residues
MEQHRRIVIKIGSSSLTNREKLLDEYKLKALASAVAKLFNQGWQPILVSSGAVAAGLGHLGWNCTHLTMPERQAAAAVGQ
GLLMEKYNELFQSLGIKVGQILLTRADFADRKRYLNARNTLNVLLNRHVLPIVNENDTVTFAEIRFGDNDTLSALVAGLV
EAELLILLTDIDGLYNGDPRKDPNAKLIPVVQHIDDSIIKLAGDSGSTVGTGGMKTKLAAARIAARSGIRTVVASAKEPS
ILSGIATGEKLGTSFEPSDHTLRSRKQWIAYGSITRGTLLIDKGAVKALTERHKSLLAVGIIGIKGDFTAGSVVSVADEQ
GVVLAKGITNLSSEDLRLVNKNTRHGLEVINRDWLVCERGFSICQM

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=33.6805555555556, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=33.448275862069, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1786437, Length=350, Percent_Identity=42.2857142857143, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=33.0882352941176, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=33.0882352941176, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320506, Length=353, Percent_Identity=39.0934844192635, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=350, Percent_Identity=38.5714285714286, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI21357643, Length=283, Percent_Identity=34.2756183745583, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39478; Mature: 39478

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQHRRIVIKIGSSSLTNREKLLDEYKLKALASAVAKLFNQGWQPILVSSGAVAAGLGHL
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHC
GWNCTHLTMPERQAAAAVGQGLLMEKYNELFQSLGIKVGQILLTRADFADRKRYLNARNT
CCCEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHHHHHHHHH
LNVLLNRHVLPIVNENDTVTFAEIRFGDNDTLSALVAGLVEAELLILLTDIDGLYNGDPR
HHHHHCCCEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
KDPNAKLIPVVQHIDDSIIKLAGDSGSTVGTGGMKTKLAAARIAARSGIRTVVASAKEPS
CCCCCCEEHHHHHCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCH
ILSGIATGEKLGTSFEPSDHTLRSRKQWIAYGSITRGTLLIDKGAVKALTERHKSLLAVG
HHHHHCCHHHHCCCCCCCCHHHHHHHHEEEECCCCCCEEEEECCHHHHHHHHHHHEEEEE
IIGIKGDFTAGSVVSVADEQGVVLAKGITNLSSEDLRLVNKNTRHGLEVINRDWLVCERG
EEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCHHHEECCCCHHHHHHHCCCEEEECCC
FSICQM
CHHHCC
>Mature Secondary Structure
MEQHRRIVIKIGSSSLTNREKLLDEYKLKALASAVAKLFNQGWQPILVSSGAVAAGLGHL
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHC
GWNCTHLTMPERQAAAAVGQGLLMEKYNELFQSLGIKVGQILLTRADFADRKRYLNARNT
CCCEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHHHHHHHHH
LNVLLNRHVLPIVNENDTVTFAEIRFGDNDTLSALVAGLVEAELLILLTDIDGLYNGDPR
HHHHHCCCEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
KDPNAKLIPVVQHIDDSIIKLAGDSGSTVGTGGMKTKLAAARIAARSGIRTVVASAKEPS
CCCCCCEEHHHHHCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHCCCCCH
ILSGIATGEKLGTSFEPSDHTLRSRKQWIAYGSITRGTLLIDKGAVKALTERHKSLLAVG
HHHHHCCHHHHCCCCCCCCHHHHHHHHEEEECCCCCCEEEEECCHHHHHHHHHHHEEEEE
IIGIKGDFTAGSVVSVADEQGVVLAKGITNLSSEDLRLVNKNTRHGLEVINRDWLVCERG
EEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCHHHEECCCCHHHHHHHCCCEEEECCC
FSICQM
CHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]