| Definition | Clostridium difficile 630 chromosome, complete genome. |
|---|---|
| Accession | NC_009089 |
| Length | 4,290,252 |
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The map label for this gene is topB [H]
Identifier: 126700019
GI number: 126700019
Start: 2771822
End: 2774050
Strand: Direct
Name: topB [H]
Synonym: CD2404
Alternate gene names: 126700019
Gene position: 2771822-2774050 (Clockwise)
Preceding gene: 126700016
Following gene: 126700021
Centisome position: 64.61
GC content: 28.49
Gene sequence:
>2229_bases ATGAAACTTGTACTTGCTGAGAAACCATCTGTGGCAAAAACTATAGCATCATTTTTAGGTGCAAAGACCAGACAAGATGG TTACTTTGAAGGAAATGATTATATAATTACTTATGCAGTTGGACACTTAGTATCATTATATGATATGAAGGATTATGATA AAGATAAATACTCTGGCTCTTGGAAAATGAACAATTTCCCATTTGTTCCTGAGGATAAGTTTAAATTTAAGATTGATAGT TCTAAAACTAAACAATTCAACACTGTAAAAAAGCTTTTGAATAGAAAAGATGTAGAATATGTAATAAATGCCACTGATAA TGACCGAGAAGGAGAACTTATAGCATTTTTAATATTTTTACTGGCAAAAAATAAAAAACCAGTAAAAAGAATCTTGGTAA ATGAATGGACACCAGAAGATATTACAAGAGGTATAAAAAACCTAAAAGATGAAGATGAAATGAGAAATCTTCAAGCTGCT GGATATACTAGGCTTATTACTGACTGGTTGATTGGAATAAATTTCACTTCTGTTGCTACATTGAAATATGGAAATGGGAA ACTATTAAACATAGGTAGGGTAATTCTTCCAACAGTTAAATTAGTCTATGATAGAGATATGGAAATACTAAACTTTGTTC CTAAAACTTATTATGAAATAGAAGGGCATTTTAAAGCTGAAGCTGGTGAATATAAAGGTAAATATGTTAAGGGTAAAGAA AGTAAATTTGATACACTTGAAGATGCAAATAAAATTATAGCTTCAATTACATCTGAAACTGGTAAAATACTAGATAAAAA AGTTACTATGTCAAAGGAATATGCACCAAAACTTTTTAGTTTGACATCACTTCAAGGATATATAACTTCTAAGTATTCCA ATTTTACATCTGATAAGGTTTTAAATGTCTGTCAATCTCTCTATGAGGGTAATGGTAAGGGTGGATACATAACTTACCCT AGAACTGATTCCATTTTCTTAGAAGAAAGTTTGGCTTCAAAAGCTTCTCAAACTTTAGATAAATTAAAGGTTGGTCTTGA ATATGAAAATAAAATTAAATTTTCTAAAACTAAAAGAGTTTTTGATTCTTCTAAAGTAGACTCCCATAGTGCTATAATTC CAACTTATATAATTCCAAAAAGTTTATCTAAGGATGAACAATTAGTTTATGATGCCATAAAAGATAGATTTGTAGCAAAT TTTATGCCTCCTGCTGAATATGAGAATACAGAAATTAAGACTGAAGTAGATAATTGTACTTTCTTAACAAAAGGTAAGGT GCTTAAATCAAAAGGATACCTTGAGGTATATAATAAGGAAGAAAAAAACGATTTACTTCCACTTGTAAATAAAGATGATG TTGTAGATGTGTTAGAAATAAAACCATTAACTAAGCAAACTACTCCACCTAAACCGTATACTGAGGACACCCTTTTAAAG GCTATGAAAAATTGTGGTAAAAATGTGCCTGAAGAAGATACAACAGTGCTATCTGGATATTCGATTGGAACATCAGCCAC ACGTGCTGATGTGTTAAAGAAGATAAGTCAAGTAGGCTATGTAAAGAAAAAAGGGAAATCTTATTCTATAACAGAACTTG GGAAAAATTTAGTTGAAATTTTTCCTGTTAAAGACTTATTTGATGTAGATTATACTGGAAAACTTGAAAAAAGTTTAAGC GATATTCAAAAAGGAAAGTATACAAGAAAAGAATATCTTACTAATATCATTAGTTTTATATATCAAAATGTCAATATTAT AAAACAAGATGCTTCAAAAAATATAAACACAGAAGCATATACTTTCGATTCAAAAACTAAAAAATTTACTAAAACAAAAG AAAAAAAACAGAAAAAGGATGTAACTAATACTGAAAAAGAAGTTGCTATATCAAAGGATTCTAATAACAAAAAAGAAGTT AAAAATGGTGATGAAAATCAATCTCTTGGCAAATGCCCCATTTGTCAAGGTGATGTACTAGAATTTGACAAAGGCTTTGC TTGTAAAAATCATAAAGAATGTAAATTTGTTATTTGGAAAAATGATAAGTATTTAAGTCTTTATAAAAAGAAAGTTAACA AAACAATGGTCAAAAATATTTTAAAAAAAGGTGAAACTAAAGTAAAATCTTTAACTGCTAAAAACGGTAATAAATTTGAT GCTACATTAAAATATGATAAAAATCCAGATACTGGCTATTTCAATTGGAAAATAGAGTTTGACAACTAA
Upstream 100 bases:
>100_bases TTCAACTTTCTATAAATATTATAACTTAAATTTCGACCTCTTGAAGTTTTTTATTTTTTTGTGGTAATATAATTATTAGT GTTTTAAAGGAGGATTTTTT
Downstream 100 bases:
>100_bases ATATTCATAAGAAAAGAAATAGCAGTAGTATAATAATCTATATTATATTACTGCTATTTCTTTTCTTATGAAGTTTTAAC TTTTTTAATAGTGACTTTTA
Product: DNA topoisomerase
Products: NA
Alternate protein names: DNA topoisomerase III [H]
Number of amino acids: Translated: 742; Mature: 742
Protein sequence:
>742_residues MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLVSLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDS SKTKQFNTVKKLLNRKDVEYVINATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYYEIEGHFKAEAGEYKGKYVKGKE SKFDTLEDANKIIASITSETGKILDKKVTMSKEYAPKLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYP RTDSIFLEESLASKASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDAIKDRFVAN FMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVNKDDVVDVLEIKPLTKQTTPPKPYTEDTLLK AMKNCGKNVPEEDTTVLSGYSIGTSATRADVLKKISQVGYVKKKGKSYSITELGKNLVEIFPVKDLFDVDYTGKLEKSLS DIQKGKYTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNTEKEVAISKDSNNKKEV KNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKNDKYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFD ATLKYDKNPDTGYFNWKIEFDN
Sequences:
>Translated_742_residues MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLVSLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDS SKTKQFNTVKKLLNRKDVEYVINATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYYEIEGHFKAEAGEYKGKYVKGKE SKFDTLEDANKIIASITSETGKILDKKVTMSKEYAPKLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYP RTDSIFLEESLASKASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDAIKDRFVAN FMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVNKDDVVDVLEIKPLTKQTTPPKPYTEDTLLK AMKNCGKNVPEEDTTVLSGYSIGTSATRADVLKKISQVGYVKKKGKSYSITELGKNLVEIFPVKDLFDVDYTGKLEKSLS DIQKGKYTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNTEKEVAISKDSNNKKEV KNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKNDKYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFD ATLKYDKNPDTGYFNWKIEFDN >Mature_742_residues MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLVSLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDS SKTKQFNTVKKLLNRKDVEYVINATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYYEIEGHFKAEAGEYKGKYVKGKE SKFDTLEDANKIIASITSETGKILDKKVTMSKEYAPKLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYP RTDSIFLEESLASKASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDAIKDRFVAN FMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVNKDDVVDVLEIKPLTKQTTPPKPYTEDTLLK AMKNCGKNVPEEDTTVLSGYSIGTSATRADVLKKISQVGYVKKKGKSYSITELGKNLVEIFPVKDLFDVDYTGKLEKSLS DIQKGKYTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNTEKEVAISKDSNNKKEV KNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKNDKYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFD ATLKYDKNPDTGYFNWKIEFDN
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=728, Percent_Identity=24.3131868131868, Blast_Score=118, Evalue=2e-26, Organism=Escherichia coli, GI1788061, Length=618, Percent_Identity=29.126213592233, Blast_Score=259, Evalue=3e-70, Organism=Escherichia coli, GI1787529, Length=625, Percent_Identity=26.08, Blast_Score=150, Evalue=2e-37, Organism=Caenorhabditis elegans, GI32563869, Length=678, Percent_Identity=24.188790560472, Blast_Score=102, Evalue=7e-22, Organism=Caenorhabditis elegans, GI17555378, Length=613, Percent_Identity=23.4910277324633, Blast_Score=92, Evalue=1e-18, Organism=Saccharomyces cerevisiae, GI6323263, Length=659, Percent_Identity=24.1274658573596, Blast_Score=108, Evalue=2e-24, Organism=Drosophila melanogaster, GI24585251, Length=721, Percent_Identity=22.1914008321775, Blast_Score=91, Evalue=2e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR005738 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 84650; Mature: 84650
Theoretical pI: Translated: 9.72; Mature: 9.72
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLVSLYDMKDYDKDKYSGS CEEEECCCCHHHHHHHHHHCCCCCCCCEECCCCEEEEEEHHHHHHHHHCCCCCCCCCCCC WKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVINATDNDREGELIAFLIFL EECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH LAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAAGYTRLITDWLIGINFTSVAT HHCCCCHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCEEEEEE LKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYYEIEGHFKAEAGEYKGKYVKGKE EEECCCCEEECCHHHHHHHHHHHCCCHHHHHHCCCCEEEECCEEEECCCCCCCCEECCCC SKFDTLEDANKIIASITSETGKILDKKVTMSKEYAPKLFSLTSLQGYITSKYSNFTSDKV CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHH LNVCQSLYEGNGKGGYITYPRTDSIFLEESLASKASQTLDKLKVGLEYENKIKFSKTKRV HHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH FDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDAIKDRFVANFMPPAEYENTEIKTEVDNCT HCCCCCCCCCCEECEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEE FLTKGKVLKSKGYLEVYNKEEKNDLLPLVNKDDVVDVLEIKPLTKQTTPPKPYTEDTLLK EEECCCEEECCCCEEECCCCCCCCCCCCCCCCCCEEHEECCCCCCCCCCCCCCCHHHHHH AMKNCGKNVPEEDTTVLSGYSIGTSATRADVLKKISQVGYVKKKGKSYSITELGKNLVEI HHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHH FPVKDLFDVDYTGKLEKSLSDIQKGKYTRKEYLTNIISFIYQNVNIIKQDASKNINTEAY CCCCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHEEHHHCCCCCCCEE TFDSKTKKFTKTKEKKQKKDVTNTEKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVL EECCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCC EFDKGFACKNHKECKFVIWKNDKYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFD CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEE ATLKYDKNPDTGYFNWKIEFDN EEEEECCCCCCCEEEEEEEECC >Mature Secondary Structure MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLVSLYDMKDYDKDKYSGS CEEEECCCCHHHHHHHHHHCCCCCCCCEECCCCEEEEEEHHHHHHHHHCCCCCCCCCCCC WKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVINATDNDREGELIAFLIFL EECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH LAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAAGYTRLITDWLIGINFTSVAT HHCCCCHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCEEEEEE LKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYYEIEGHFKAEAGEYKGKYVKGKE EEECCCCEEECCHHHHHHHHHHHCCCHHHHHHCCCCEEEECCEEEECCCCCCCCEECCCC SKFDTLEDANKIIASITSETGKILDKKVTMSKEYAPKLFSLTSLQGYITSKYSNFTSDKV CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHH LNVCQSLYEGNGKGGYITYPRTDSIFLEESLASKASQTLDKLKVGLEYENKIKFSKTKRV HHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH FDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDAIKDRFVANFMPPAEYENTEIKTEVDNCT HCCCCCCCCCCEECEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEE FLTKGKVLKSKGYLEVYNKEEKNDLLPLVNKDDVVDVLEIKPLTKQTTPPKPYTEDTLLK EEECCCEEECCCCEEECCCCCCCCCCCCCCCCCCEEHEECCCCCCCCCCCCCCCHHHHHH AMKNCGKNVPEEDTTVLSGYSIGTSATRADVLKKISQVGYVKKKGKSYSITELGKNLVEI HHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHH FPVKDLFDVDYTGKLEKSLSDIQKGKYTRKEYLTNIISFIYQNVNIIKQDASKNINTEAY CCCCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHEEHHHCCCCCCCEE TFDSKTKKFTKTKEKKQKKDVTNTEKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVL EECCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCC EFDKGFACKNHKECKFVIWKNDKYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFD CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEE ATLKYDKNPDTGYFNWKIEFDN EEEEECCCCCCCEEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]