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The map label for this gene is dosC [H]

Identifier: 126699861

GI number: 126699861

Start: 2595625

End: 2596800

Strand: Direct

Name: dosC [H]

Synonym: CD2244

Alternate gene names: 126699861

Gene position: 2595625-2596800 (Clockwise)

Preceding gene: 126699843

Following gene: 126699868

Centisome position: 60.5

GC content: 28.49

Gene sequence:

>1176_bases
ATGAACTTAGAAAAAATAAAAAAATTTTTTGGATTTCCTCTAGACAAAAAACACAAAGACAATGTTGAGAAAGAACTCTG
CAATTATCTTTATCATACGGGTAAAATCATTTGCATAATAATAGTAATATCTCAAATAATTATGATGATATCAATCTCAG
CTAGAGAAGGAGGCCCTTTTGTCACACCAAGACGCCAATGGTATTTTTATATGTATGTAACACTATTTTGTGTATCTTCT
GTCTTTATGTTTGTATTTTCTTATCTTTGGAATAAAAAAAAGGATTGCTGTACGTTTTATATAAATTTATGGATAATTTA
TGTGACTTTTTTCTGCCTATGGGGTTGTGTAATTACTCTACTTGACCAGTTAGGTGGAAATGACCTAAGTGTATTTAGTT
ATATTATGTTAGCAGCTGCAGCTTTCTCAGTATTAAAATTTGGAGAATCAGTAATAGTTTTTGGAGGATGCTTTTTGTTT
CTTAATTTATTCTTACCTGGGATTCAAACTATACCAGGCAATGAGTTTAGTAATATTGTCAACACATTTTTTATAACATC
CCTTGCCATTTTTGTTTCTACAATGCTATATAGAAATCGTGTATTTACTTACTATGATAAACTTTTAATTAGTCAACAAT
ATGAAGAAATATCTCATATAAATAATTTACTCAACGACAAAGTTATGACAGATGACCTTACTAAAATGAACAACCGTAGA
TACCTTGAAGATATTCTACAAAAAAAATTAATCAGCCATGCAAATCAAGAACCTATAACTGGAATGATGATAGATATAGA
TTATTTTAAACAATTTAATGATAATTATGGACATCAAAAGGGAGACTATTGTCTACAAAATATTGCCAAAGTAATTTTAG
ATTTTACAAGTTCTGAAAATGAACATGCTATACGATATGGTGGTGAGGAATTTTTTATATGTATTTTTAACTGTAATGAG
GCAGATGCTAAACTTAAATCAGAATGTTTACGAAAAATTATAGAGTCCTGCAATTTTGAGCGTGATGATGTTCCTTTAAA
ATGTGTAACTGTTAGTATTGGAGTTTATACAAAGAAAATTGAAAAGACAATAACATTAAATGAGTTTATAAGTAATTGTG
ATAAGGCTCTATATACTGCAAAAGAACAAGGACGTAATTGTGTTGCATTTTATTAA

Upstream 100 bases:

>100_bases
AATATATTTTGATATAATTAAATCATAAACGAAGTATTCCAAATATCTAATGATAGAAAGGAATACTTATTTTTATGCTT
TATTTTTAATGGAGGCTTAT

Downstream 100 bases:

>100_bases
AAGGTTACATACTATTACATAAACCTATAAGTATTATATCGCAAAATAAAAGTAATCAAATTATATATTTATAGTACAAT
AAAAATAACTTGCATATCTC

Product: signaling protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVISQIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSS
VFMFVFSYLWNKKKDCCTFYINLWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEISHINNLLNDKVMTDDLTKMNNRR
YLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNYGHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNE
ADAKLKSECLRKIIESCNFERDDVPLKCVTVSIGVYTKKIEKTITLNEFISNCDKALYTAKEQGRNCVAFY

Sequences:

>Translated_391_residues
MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVISQIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSS
VFMFVFSYLWNKKKDCCTFYINLWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEISHINNLLNDKVMTDDLTKMNNRR
YLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNYGHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNE
ADAKLKSECLRKIIESCNFERDDVPLKCVTVSIGVYTKKIEKTITLNEFISNCDKALYTAKEQGRNCVAFY
>Mature_391_residues
MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVISQIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSS
VFMFVFSYLWNKKKDCCTFYINLWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEISHINNLLNDKVMTDDLTKMNNRR
YLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNYGHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNE
ADAKLKSECLRKIIESCNFERDDVPLKCVTVSIGVYTKKIEKTITLNEFISNCDKALYTAKEQGRNCVAFY

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=185, Percent_Identity=31.8918918918919, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=35, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87081881, Length=195, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=31.7647058823529, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=161, Percent_Identity=34.1614906832298, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082007, Length=237, Percent_Identity=26.1603375527426, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=29.1139240506329, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45492; Mature: 45492

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVISQIIMMISISAREGGPF
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYINLWIIYVTFFCLWGCVITL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLFLNLFLPGIQTIPGNEFSNIV
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
NTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEISHINNLLNDKVMTDDLTKMNNRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHH
YLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNYGHQKGDYCLQNIAKVILDFTSSEN
HHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EHAIRYGGEEFFICIFNCNEADAKLKSECLRKIIESCNFERDDVPLKCVTVSIGVYTKKI
CCCEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHH
EKTITLNEFISNCDKALYTAKEQGRNCVAFY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVISQIIMMISISAREGGPF
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYINLWIIYVTFFCLWGCVITL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLFLNLFLPGIQTIPGNEFSNIV
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
NTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEISHINNLLNDKVMTDDLTKMNNRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHH
YLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNYGHQKGDYCLQNIAKVILDFTSSEN
HHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EHAIRYGGEEFFICIFNCNEADAKLKSECLRKIIESCNFERDDVPLKCVTVSIGVYTKKI
CCCEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHH
EKTITLNEFISNCDKALYTAKEQGRNCVAFY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]