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The map label for this gene is sat [H]

Identifier: 126699843

GI number: 126699843

Start: 2576453

End: 2577085

Strand: Direct

Name: sat [H]

Synonym: CD2226

Alternate gene names: 126699843

Gene position: 2576453-2577085 (Clockwise)

Preceding gene: 126699842

Following gene: 126699861

Centisome position: 60.05

GC content: 28.28

Gene sequence:

>633_bases
ATGACTATTCCAAGTTCAAATAAGGTATATCCAAGAAGTAATGATTATCAAACTGTATATCTTAAAAATGTAATAACAAG
GGAAAATATAGAAGTTGGAGATTACACAATATATAATGACTTTTATAATGACCCAAGAGATTTTGAGAAAAACAATGTAT
TGTATCATTACCCTATAAACAATGATAAATTAATAATTGGTAAGTTTTGCTCAATAGCATGTAATGCAAAATTTCTAATG
ACTAGTGGAAATCACTCAATGAAATCAAAATCCACATATACTTTTCCCATCTTTTATGAAGAATGGAAACTCGATATCAA
CCATATAACGGACGCTTGGGACAATAAGGGTAACATTATAATTGGAAATGATGTGTGGATAGGTTATGATTCTATAATTA
TGTCAGGTGTAAATATTGGTGATGGTGCAATTATTGGAACAAGAGCAGTAGTTACTAATGACGTTCCACCGTATTCTATT
GTTGGAGGAGTACCTGCAAAAATTATAAAAAAGAGATTTAATAATGATACAATTTCAAAATTATTAGAAATTAAATGGTG
GGATTGGCCATATAAAAAAATTCAATCAAACATTAAGCATATTCAATCAGGAAATATTGATAAATTAGTTTAA

Upstream 100 bases:

>100_bases
ATCTTATGAAGATTCTTAACAAGGAGTGATTCTGTGAAGAGATGTATTGTAGTAGTGGAATAGTCAAAAACATAATATAT
ATTAAAGGAGAGATTTAAAT

Downstream 100 bases:

>100_bases
TATTTTATAATTTATTTATATTAAACTTTACTTTAATAATCATTTTATAAGAGCTTTTTCAAAATTTGTATATTATTTTA
ATATATATAAATTTAATACA

Product: streptogramin A acetyltransferase

Products: CoA; acetylmaltose [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLM
TSGNHSMKSKSTYTFPIFYEEWKLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV

Sequences:

>Translated_210_residues
MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLM
TSGNHSMKSKSTYTFPIFYEEWKLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV
>Mature_209_residues
TIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMT
SGNHSMKSKSTYTFPIFYEEWKLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSIV
GGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 24143; Mature: 24012

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYHYPIN
CCCCCCCEECCCCCCCEEEEEEEEEECCCCEECCEEEECCCCCCCCCCCCCCEEEEECCC
NDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEWKLDINHITDAWDNKGNII
CCCEEEEEEEEEEECCEEEEECCCCCCCCCCEEEEEEEEEEEEEEHHHHCCCCCCCCCEE
IGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSIVGGVPAKIIKKRFNNDTISK
ECCCEEECHHHHHEECCCCCCCEEEECEEEEECCCCCEEEECCCHHHHHHHHCCCHHHHH
LLEIKWWDWPYKKIQSNIKHIQSGNIDKLV
HHEEEECCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYHYPIN
CCCCCCEECCCCCCCEEEEEEEEEECCCCEECCEEEECCCCCCCCCCCCCCEEEEECCC
NDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEWKLDINHITDAWDNKGNII
CCCEEEEEEEEEEECCEEEEECCCCCCCCCCEEEEEEEEEEEEEEHHHHCCCCCCCCCEE
IGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSIVGGVPAKIIKKRFNNDTISK
ECCCEEECHHHHHEECCCCCCCEEEECEEEEECCCCCEEEECCCHHHHHHHHCCCHHHHH
LLEIKWWDWPYKKIQSNIKHIQSGNIDKLV
HHEEEECCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 0.018 {acetyl-CoA}} 62 {glucose}} 90 {maltose}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]