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The map label for this gene is thiH [H]

Identifier: 126699312

GI number: 126699312

Start: 1979561

End: 1980664

Strand: Direct

Name: thiH [H]

Synonym: CD1705

Alternate gene names: 126699312

Gene position: 1979561-1980664 (Clockwise)

Preceding gene: 126699311

Following gene: 126699313

Centisome position: 46.14

GC content: 29.62

Gene sequence:

>1104_bases
ATGAGTTTTTATGATGTAATAGAAAAATATAGGGATTTTGATTTTGATGGATATTTAAATAATGTTACTGATAATGATGT
TTTAAGAAGTTTATCAAAAGACAAGCTTGAAGATTTTGATATATTAAATTTGCTTTCTAAAACTGCAGTAAAACATTTAG
AAGATATGGCACAAAAAGCACATAAATTAAGTGTTCAATACTTTGGTAAAACAGTATGTTTATATACACCAATGTATATA
GCAAATTATTGTGTTAATCAATGTGTTTATTGTAGCTATAATATAAAGAGTGGAATAAAAAGAAAAAAATTAACTATGGA
TGAAATTAGAGAAGAGGGAGAAGCAATTTCAAAAGAAGGTTTCAAACATCTACTTGTTTTGACTGGAGAAAGTAGTTTTC
ACTCAAGTGTAGAATATATAGGTGAGGCTATAGAAATATTAAGAGAGAAATTTCCCTCTATAGGTATAGAAGTTTATCCA
ATGGAAGTAGAAGAATATAAGTACATTGTAGATAAAGGTGTTGAAGGATTAACAGTATATCAAGAAACTTATGACGAGGA
AATATATAAGAGAGTACATATAAAAGGCCCAAAATCAAATTATAAATTTAGACTAGATGCTCCTGAAAGAGGGGCAAAAG
CAGGTATGAGGACTTTATCAATAGGTGCACTTTTAGGGCTAAATGATTTTAGAAAAGAAACTTTTTTTACAATATTGCAT
GGGAAATACTTAAAAACGAAATATCCTCACATTGAGCTTTCATATTCAACACCAAGAATGAGACCTTTTAAAGGGTGTTT
TGAGGAATTGGTAGATATAAGTGATACTGATTTAGTTCAAGCTATGGTATGTATGAGATTATTTGACCCTCATGCAGCTA
TAAACATATCCACTAGAGAAAATTTAGAAATGAGAAGTCATATAATACCTTTAGGAGTAACTAAATTGAGTGCAGGTGTA
TCTACTGATGTAGGAGGTCATTCTCAAGATGAGCATGATACTGCTCAATTTAAGATAAATGATGAAAGTACTGTTAAAGA
TGTGGAGAAAATGCTAAACTCAATTGGTTATCAGCATGTGTTTAAAGATTGGGAAAGATTTTAA

Upstream 100 bases:

>100_bases
GAAGCATATATTGCAAAAACAGGAAATGTGTCAGAATTTGCAAATGCATCTTCTCCTTTAACAGGTTTTTTAGGCAATTT
ATAGAAAGTGGTGATTGGTG

Downstream 100 bases:

>100_bases
ATGTATCTGATAACAAACAGAAAATTATGTAGTGAGGAACGATATTTAGAAGTTATTAAAGAGTCTATATTGAGTGGTGT
AGAAAATATAATAATAAGAG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNLLSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYI
ANYCVNQCVYCSYNIKSGIKRKKLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRTLSIGALLGLNDFRKETFFTILH
GKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDTDLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGV
STDVGGHSQDEHDTAQFKINDESTVKDVEKMLNSIGYQHVFKDWERF

Sequences:

>Translated_367_residues
MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNLLSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYI
ANYCVNQCVYCSYNIKSGIKRKKLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRTLSIGALLGLNDFRKETFFTILH
GKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDTDLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGV
STDVGGHSQDEHDTAQFKINDESTVKDVEKMLNSIGYQHVFKDWERF
>Mature_366_residues
SFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNLLSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIA
NYCVNQCVYCSYNIKSGIKRKKLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYPM
EVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRTLSIGALLGLNDFRKETFFTILHG
KYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDTDLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVS
TDVGGHSQDEHDTAQFKINDESTVKDVEKMLNSIGYQHVFKDWERF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=364, Percent_Identity=36.2637362637363, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42312; Mature: 42181

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNLLSKTAVKHLEDMAQKA
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRKKLTMDEIREEGEAISKEG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEECHHHCCHHHCCCHHHHHHHHHHHHHCC
FKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYPMEVEEYKYIVDKGVEGLTVY
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEHH
QETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRTLSIGALLGLNDFRKETFFTILH
HHHHHHHHHHHEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDTDLVQAMVCMRLFDPHAAINISTRE
CCHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCHH
NLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDTAQFKINDESTVKDVEKMLNSIGYQHV
CHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCHHHH
FKDWERF
HHHHHCC
>Mature Secondary Structure 
SFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNLLSKTAVKHLEDMAQKA
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRKKLTMDEIREEGEAISKEG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEECHHHCCHHHCCCHHHHHHHHHHHHHCC
FKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYPMEVEEYKYIVDKGVEGLTVY
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEHH
QETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRTLSIGALLGLNDFRKETFFTILH
HHHHHHHHHHHEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDTDLVQAMVCMRLFDPHAAINISTRE
CCHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCHH
NLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDTAQFKINDESTVKDVEKMLNSIGYQHV
CHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCHHHH
FKDWERF
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]