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The map label for this gene is thiG

Identifier: 126699311

GI number: 126699311

Start: 1978774

End: 1979544

Strand: Direct

Name: thiG

Synonym: CD1704

Alternate gene names: 126699311

Gene position: 1978774-1979544 (Clockwise)

Preceding gene: 126699310

Following gene: 126699312

Centisome position: 46.12

GC content: 34.5

Gene sequence:

>771_bases
ATGGATAAATTGGTACTTGGTGGACATGAATTTAATAGCAGATTATTGGTTGGAACAGGTAAATATGGTTCAAATAATAT
ATTGCCTGAGGTAATAAAAGAAAGTGGAAGTGAAATAATAACAATGGCACTTAGAAGAGTGGATTTAGACAATAAACAAG
AAAACATATTAACGTATATTCCAAAAGAGATGACAATACTTCCAAATACATCAGGAGCTACTAATGCAGAAGAAGCTGTA
AGAATAGCTAGAATATCTAGAAAAATGGGATGTGGAGATTTTATAAAAATAGAAGTAATATCAGATACTAGATATTTACT
TCCTGATAATGAAGAAACTATAAAAGCTACAAAGATACTTGCTGATGAGGGGTTTATAGTTCTTCCATATATGACACCAG
ACCTTTATGCTGGAAGAAGACTTATAGAAGCTAATGCAGCGGCAGTTATGCCACTTGGAGCTCCAATTGGTTCAAATAGA
GGTCTTCAAATGAAAGAAATGATAAGAATAATGATAGATGAGTTGGATATACCAATAATAGTAGATGCAGGTATTGGAAA
ACCATCTCAAGCTATGGAGGCTATGGAAATGGGAGCTGATGCAGTACTTGTCAACACTGCTATAGCTTCAGCAGGAGACC
CTGTACAAATGGCTAGAGCATTTAAATTAGCTGTAGAAGGTGGAAGAGAAGCATATATTGCAAAAACAGGAAATGTGTCA
GAATTTGCAAATGCATCTTCTCCTTTAACAGGTTTTTTAGGCAATTTATAG

Upstream 100 bases:

>100_bases
TGGCAAATTTGGCAACGCAAATACTTATAGATATGTAAATATTAAAATTAGATATAAAAAAGATTGAATATTAAAAAAAT
TTAACGTGGAGGGAAATAAT

Downstream 100 bases:

>100_bases
AAAGTGGTGATTGGTGATGAGTTTTTATGATGTAATAGAAAAATATAGGGATTTTGATTTTGATGGATATTTAAATAATG
TTACTGATAATGATGTTTTA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMALRRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAV
RIARISRKMGCGDFIKIEVISDTRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMARAFKLAVEGGREAYIAKTGNVS
EFANASSPLTGFLGNL

Sequences:

>Translated_256_residues
MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMALRRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAV
RIARISRKMGCGDFIKIEVISDTRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMARAFKLAVEGGREAYIAKTGNVS
EFANASSPLTGFLGNL
>Mature_256_residues
MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMALRRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAV
RIARISRKMGCGDFIKIEVISDTRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMARAFKLAVEGGREAYIAKTGNVS
EFANASSPLTGFLGNL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=244, Percent_Identity=52.4590163934426, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_CLOD6 (Q186R1)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001088208.1
- ProteinModelPortal:   Q186R1
- SMR:   Q186R1
- STRING:   Q186R1
- GeneID:   4916744
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1704
- NMPDR:   fig|1496.1.peg.1667
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27558; Mature: 27558

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMALRRVDLDNKQENILTYI
CCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEC
PKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISDTRYLLPDNEETIKATKIL
CCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCCCCEEEEEEE
ADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNRGLQMKEMIRIMIDELDIPII
ECCCEEEEEECCCCHHHCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEE
VDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMARAFKLAVEGGREAYIAKTGNVS
EECCCCCHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHH
EFANASSPLTGFLGNL
HHHCCCCCHHHHHCCC
>Mature Secondary Structure
MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMALRRVDLDNKQENILTYI
CCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEC
PKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISDTRYLLPDNEETIKATKIL
CCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEECCCCCCCEEEEEEE
ADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNRGLQMKEMIRIMIDELDIPII
ECCCEEEEEECCCCHHHCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEE
VDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMARAFKLAVEGGREAYIAKTGNVS
EECCCCCHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHH
EFANASSPLTGFLGNL
HHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA