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The map label for this gene is thiE2 [H]

Identifier: 126699313

GI number: 126699313

Start: 1980665

End: 1981354

Strand: Direct

Name: thiE2 [H]

Synonym: CD1706

Alternate gene names: 126699313

Gene position: 1980665-1981354 (Clockwise)

Preceding gene: 126699312

Following gene: 126699314

Centisome position: 46.17

GC content: 22.32

Gene sequence:

>690_bases
ATGTATCTGATAACAAACAGAAAATTATGTAGTGAGGAACGATATTTAGAAGTTATTAAAGAGTCTATATTGAGTGGTGT
AGAAAATATAATAATAAGAGAAAAGGATTTGGAATATCAAGAGCTAAGAAAATTATATATGAAAATAAAAACTAAAATCA
ATTGTATAGATTTTCAAGAGCAAATATCAGATGAAAGTTTGAAAACTAATATAAATCAAAAAGAGTGTAGAAATAAATTC
AAGGTTAATTTTATAATAAATAGTAACATTGAATTTTTTGAAAAAGTGGATTGTCAAGGTATCCATCTTCCTTTTAAATT
ATTCTTGAATTTAATTGAAAATAAGTATAATTTTAATGAAAATAAAATACTAGGATTATCTTTACATAAAGTAGAGGAAG
TAGATTATTTAGAAAAATTAATAAGAAATCAAAATATAAAAATAGATTACATAACATTATCACATATATATGAAACTAAA
TGTAAAGAAGGATTAAATCCAAAAGGAATTGAGCTTTTAAAAGAAGCTAAAAAGATTACTGATATTAAAATAATTGCACT
TGGGGGAATTTTACCATCTAATGTCAAGGAAACATTAAAATACTGTGATGATTTTGCTATAATGTCAACTATAATGAGGT
CTAAAGATATTAAAAAAACCATATCAAATTATAATGAAAAGTTGAATTAG

Upstream 100 bases:

>100_bases
TCAATTTAAGATAAATGATGAAAGTACTGTTAAAGATGTGGAGAAAATGCTAAACTCAATTGGTTATCAGCATGTGTTTA
AAGATTGGGAAAGATTTTAA

Downstream 100 bases:

>100_bases
TAAATATAGTATATTGAATTTTTAACTTAAATAAAAATAAAAAAGTTATCTTTTAAATTTTAAAAGGTAGCTTTTTTATT
TTATATCTTTGCTAATAAAA

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRKLYMKIKTKINCIDFQEQISDESLKTNINQKECRNKF
KVNFIINSNIEFFEKVDCQGIHLPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIKKTISNYNEKLN

Sequences:

>Translated_229_residues
MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRKLYMKIKTKINCIDFQEQISDESLKTNINQKECRNKF
KVNFIINSNIEFFEKVDCQGIHLPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIKKTISNYNEKLN
>Mature_229_residues
MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRKLYMKIKTKINCIDFQEQISDESLKTNINQKECRNKF
KVNFIINSNIEFFEKVDCQGIHLPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIKKTISNYNEKLN

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 27014; Mature: 27014

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRKLYMKIKTKINCIDFQE
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEECHHH
QISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIHLPFKLFLNLIENKYNFNE
HHCCHHHHCCCCHHHHCCCEEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
NKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETKCKEGLNPKGIELLKEAKKIT
CEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIKKTISNYNEKLN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRKLYMKIKTKINCIDFQE
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEECHHH
QISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIHLPFKLFLNLIENKYNFNE
HHCCHHHHCCCCHHHHCCCEEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
NKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETKCKEGLNPKGIELLKEAKKIT
CEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIKKTISNYNEKLN
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]