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The map label for this gene is thiE1

Identifier: 126699205

GI number: 126699205

Start: 1856405

End: 1857037

Strand: Direct

Name: thiE1

Synonym: CD1601

Alternate gene names: 126699205

Gene position: 1856405-1857037 (Clockwise)

Preceding gene: 126699204

Following gene: 126699206

Centisome position: 43.27

GC content: 29.54

Gene sequence:

>633_bases
ATGATTGATAAAGAATCTTTAAAAAAATGTTTAAAGTTGTATTTAGTAACTGACTCTGAAATGCTTAAAGGTAGAGACTT
TTATAAATGTCTTGAAGATGCAATATCTTCTGGAATAACAACTGTACAACTTAGAGAAAAAAATGCTTCTGGAAGAGAGT
TTTTAAGAAAAGCTATGAAACTTAGAGAAATAACTAAAAGATATGGTGTTAAGTTTATAATAAATGATAGAGTGGATATA
GCACTTATTTGTGATGCAGATGGAGTACATGTAGGACAGAGTGACATTGATGTTAGAGAGGTTAGAAAGCTTATAGGAAA
CAATAAAATTCTAGGAGTATCAGCAAGAACTTTAGAAGAAGCTATTTGTGCAAAAAATGATGGCGCAGACTATTTAGGTG
TAGGGTCTATATTCACAACATCTACTAAATTAGATGCAAAATCAGCTTCGTTTGAAACTGTTAAAGAAATTAAAGAAAAA
GTAGACATGCCATTTGTACTAATTGGAGGTATAAATTTAGACAATATAGATAAACTTAAATGTTTAGAAAGTGACGGGTA
TGCTATAATATCAGCTATATTAAAAGCTGAAGATATCTCTAAAGAAGTGGAAAAGTGGACATTAAAAATATAA

Upstream 100 bases:

>100_bases
GAAAATCTTATGAATGCTATTTATCAAATGGATATAGATGCTTTAAGTAAAAATTCTAATATTGAATTTTTAAATTAATA
AAGAGTAAGGGAGACTTAAA

Downstream 100 bases:

>100_bases
AATTGGCTCTGGTAGTGAAAAAAATGGAAAAATTTGATATAATCTAAGAGTAAAATTTAAGCAATTATAAATGATAAACT
TTTCTACATATACTTTTTAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQLREKNASGREFLRKAMKLREITKRYGVKFIINDRVDI
ALICDADGVHVGQSDIDVREVRKLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI

Sequences:

>Translated_210_residues
MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQLREKNASGREFLRKAMKLREITKRYGVKFIINDRVDI
ALICDADGVHVGQSDIDVREVRKLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI
>Mature_210_residues
MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQLREKNASGREFLRKAMKLREITKRYGVKFIINDRVDI
ALICDADGVHVGQSDIDVREVRKLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=188, Percent_Identity=28.1914893617021, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=206, Percent_Identity=40.2912621359223, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CLOD6 (Q186F8)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001088102.1
- ProteinModelPortal:   Q186F8
- STRING:   Q186F8
- GeneID:   4913386
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1601
- NMPDR:   fig|1496.1.peg.3316
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   QRDGADY
- ProtClustDB:   CLSK2534956
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23415; Mature: 23415

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQLREKNASGREFLRKAMK
CCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
LREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVRKLIGNNKILGVSARTLEE
HHHHHHHHCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHH
AICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEKVDMPFVLIGGINLDNIDKLK
HHHCCCCCCCEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
CLESDGYAIISAILKAEDISKEVEKWTLKI
EECCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQLREKNASGREFLRKAMK
CCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
LREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVRKLIGNNKILGVSARTLEE
HHHHHHHHCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHH
AICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEKVDMPFVLIGGINLDNIDKLK
HHHCCCCCCCEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
CLESDGYAIISAILKAEDISKEVEKWTLKI
EECCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA