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The map label for this gene is thiK

Identifier: 126699204

GI number: 126699204

Start: 1855594

End: 1856382

Strand: Direct

Name: thiK

Synonym: CD1600

Alternate gene names: 126699204

Gene position: 1855594-1856382 (Clockwise)

Preceding gene: 126699203

Following gene: 126699205

Centisome position: 43.25

GC content: 29.28

Gene sequence:

>789_bases
ATGTATAATTTAATTAAAGATGTAAAAAAATTAAATCCATTGGTAATTCATTATACTAATAATGTAACTATAAATGATTG
TGCTAATGTAACTTTAGCTGTAGGAGCAAGTCCTCTTATGAGTTTTTCATATGAGGAAGTAGAAGAAATGGTAAGTGTAG
CAAACTCTGTAGTAATAAATATAGGAACTATGAACTCAAATATGTTGGATTTATTCCTATTAGCTGGAAAAGCAGCAAAT
AAGTACAATAAACCTGTTGTTTTGGACCCAGTTGGAGTATTTGCAAGCAAAGCGAGAGCAGAACTTACAAGTAGACTTTT
AAATGAAGTCAAATTTAGTGTTGTTAAAGGGAATGTATCAGAAATAAAGTTTATAGGTGGCTTTAATGTAAGGGGTAAAG
GTGTAGATTCATTTGATGAAGAAGAAGATTCTACTGAGATAATAAGAAAGATTGCTGAAAAGCTTGAATGTGTAGTTGTA
GCAACTGGAAAAATAGATATAATAACTAATGGTAAAGGTACATATAAAATAAACAATGGAACTGATAAATTAAAAGGAAT
TACAGGAACTGGATGTATGACTGCTAGCTTGATTGCAAGTTTTATGGCTGTTACTGAGAATATATTAGAAGCAGCAACAA
TGGGTGTTCTTACAATGTCTTTAAGTGGGGAATTAGCTAATTTAAATAATCCACCAATAGGTACATTTAAAGAAAATCTT
ATGAATGCTATTTATCAAATGGATATAGATGCTTTAAGTAAAAATTCTAATATTGAATTTTTAAATTAA

Upstream 100 bases:

>100_bases
TAACAGAAGCAATAAAAAGAAGTTTTGATATAGGTCATGGAGTAGGACCAGTACATCATTTTTATAAATTTGAGTAATAA
AAAAATATTGGAGGTAGAAT

Downstream 100 bases:

>100_bases
TAAAGAGTAAGGGAGACTTAAAATGATTGATAAAGAATCTTTAAAAAAATGTTTAAAGTTGTATTTAGTAACTGACTCTG
AAATGCTTAAAGGTAGAGAC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSYEEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAAN
KYNKPVVLDPVGVFASKARAELTSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLTMSLSGELANLNNPPIGTFKENL
MNAIYQMDIDALSKNSNIEFLN

Sequences:

>Translated_262_residues
MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSYEEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAAN
KYNKPVVLDPVGVFASKARAELTSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLTMSLSGELANLNNPPIGTFKENL
MNAIYQMDIDALSKNSNIEFLN
>Mature_262_residues
MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSYEEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAAN
KYNKPVVLDPVGVFASKARAELTSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLTMSLSGELANLNNPPIGTFKENL
MNAIYQMDIDALSKNSNIEFLN

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=239, Percent_Identity=30.1255230125523, Blast_Score=123, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325042, Length=275, Percent_Identity=33.0909090909091, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_CLOD6 (Q186F4)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001088101.1
- ProteinModelPortal:   Q186F4
- SMR:   Q186F4
- STRING:   Q186F4
- GeneID:   4913385
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1600
- NMPDR:   fig|1496.1.peg.3317
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   TISESTY
- ProtClustDB:   CLSK2534955
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28291; Mature: 28291

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: BINDING 40-40 BINDING 116-116 BINDING 162-162 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSYEEVEEMVSVANSVVIN
CCHHHHHHHHCCCEEEEEECCEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHHCEEEEE
IGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAELTSRLLNEVKFSVVKGNVS
EECCCCCHHHHHEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
EIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVVATGKIDIITNGKGTYKINNG
HEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEECCEEEEEECCCEEEEECCC
TDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLTMSLSGELANLNNPPIGTFKENL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHH
MNAIYQMDIDALSKNSNIEFLN
HHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSYEEVEEMVSVANSVVIN
CCHHHHHHHHCCCEEEEEECCEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHHCEEEEE
IGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAELTSRLLNEVKFSVVKGNVS
EECCCCCHHHHHEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
EIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVVATGKIDIITNGKGTYKINNG
HEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEECCEEEEEECCCEEEEECCC
TDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLTMSLSGELANLNNPPIGTFKENL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHH
MNAIYQMDIDALSKNSNIEFLN
HHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA