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The map label for this gene is kipI [H]

Identifier: 126698989

GI number: 126698989

Start: 1605002

End: 1605697

Strand: Direct

Name: kipI [H]

Synonym: CD1386

Alternate gene names: 126698989

Gene position: 1605002-1605697 (Clockwise)

Preceding gene: 126698988

Following gene: 126698990

Centisome position: 37.41

GC content: 30.32

Gene sequence:

>696_bases
ATGGAAACTAGATACTTGATATCAGGGGATAAGGCAGTGGTTGCTGAATTTGGAAATGAGATATCAGAAGATATAAACAA
AAAAGTAATTTCATTTATGAGAGCAATAGAAATATCGAATCTTAAAGGTATTGTAACAGAAATGGTACCAACATATAGGT
CACTTATGATAAGTTATAATCCATTAGAAATAGATTTTGATAGTTTGATTGAGAATTTAAAAAAAATAGAAGATAATTTG
GAAAGTATAGTATTACCAAAACCTAAAATACATGAGATTCCAGTATGTTATGATGAGGTTTTTGGTATAGATATTAAAAA
TGTTGCAAGCTATAATAATCTCACTGTAGATGAAGTTATAAAAATACACACAAGCAGAGAGTACTTGATATATATGCTAG
GGTTTACTCCTGGATTTCCATATCTAGGTGGTATGGATGAAAGAATAGCTACACCTAGATTAGAAGTGCCAAGGACTAAA
ATTTATGGTGGGAGTGTTGGAATAGCAGGTTCCCAGACTGGTGTATATCCTATTGATAGTCCTGGTGGATGGCAAATAAT
TGGTAGGACACCTTTAAAATTATATGATGAAAATAGAGAAGAACAGATTTTGCTTAGAGCTGGAGATTTTATAAAATTTG
TTCCAATAACATTAGATGAATTTATAGAAATCGAAAAAAATAACTTAAGTATTTAA

Upstream 100 bases:

>100_bases
ATATTTGGATTAATAGTAGTTTTAATATCTGCATATACAGGAATAACTTCTCTTAGCAGCCTAGGTGCTTTATTTGCTTA
ATTTAGAAGAGGTGACATTT

Downstream 100 bases:

>100_bases
ATATCGTTATAGTACAGGGAGTGGTATATATGGGATTCAAAGTTGAATTAGGAGGTTTCCAAACATTAATACAAGACAGA
GGTAGAGTTGGCTATGGTCA

Product: allophanate hydrolase subunit 1

Products: CO2; NH3

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGIVTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNL
ESIVLPKPKIHEIPVCYDEVFGIDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITLDEFIEIEKNNLSI

Sequences:

>Translated_231_residues
METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGIVTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNL
ESIVLPKPKIHEIPVCYDEVFGIDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITLDEFIEIEKNNLSI
>Mature_231_residues
METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGIVTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNL
ESIVLPKPKIHEIPVCYDEVFGIDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITLDEFIEIEKNNLSI

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=213, Percent_Identity=41.3145539906103, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: 3.5.1.54

Molecular weight: Translated: 26142; Mature: 26142

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGIVTEMVPTYRSLMISYN
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECC
PLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFGIDIKNVASYNNLTVDEVI
CCEECHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHCCCHHHHCCCCCCCHHHHH
KIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTKIYGGSVGIAGSQTGVYPIDS
HHCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCEEEECCCEECCCCCCCCEECCC
PGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITLDEFIEIEKNNLSI
CCCEEEEECCCCEEECCCCCCEEEEEECCEEEEECCCHHHHHEECCCCCCC
>Mature Secondary Structure
METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGIVTEMVPTYRSLMISYN
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECC
PLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFGIDIKNVASYNNLTVDEVI
CCEECHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHCCCHHHHCCCCCCCHHHHH
KIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTKIYGGSVGIAGSQTGVYPIDS
HHCCCCEEEEEEEECCCCCCCCCCCCHHHCCCCCCCCCEEEECCCEECCCCCCCCEECCC
PGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITLDEFIEIEKNNLSI
CCCEEEEECCCCEEECCCCCCEEEEEECCEEEEECCCHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: urea-1-carboxylate; H2O

Specific reaction: urea-1-carboxylate + H2O = 2 CO2 + 2 NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]